Recovery and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography

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dc.contributor.authorSantos, Everaldo Silvino dos
dc.contributor.authorSousa Junior, Francisco Caninde de
dc.contributor.authorVaz, Michelle Rossana Ferreira
dc.contributor.authorPadilha, Carlos Eduardo de Araújo
dc.contributor.authorChibério, Abimaelle Silva
dc.contributor.authorMartins, Daniella Regina Arantes
dc.contributor.authorMacedo, Gorete Ribeiro de
dc.date.accessioned2021-05-10T19:50:34Z
dc.date.available2021-05-10T19:50:34Z
dc.date.issued2015-04-01
dc.description.resumoVisceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. However, there is currently no vaccine for human use. The aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from unclarified Escherichia coli feedstock through expanded bed adsorption (EBA) chromatography. Batch experiments were performed to optimize the adsorption and elution conditions of the antigen onto a STREAMLINETM Chelating resin using two central composite rotatable designs (CCRD). The results showed that the optimal binding con- ditions of the 503 antigen were pH 8.0 in the presence of 2.4 M NaCl. For the elution of the target protein, the optimized conditions included the presence of 600.0 mM imidazole. The adsorption isothermal data of the 503 antigen were fitted to the Langmuir adsorption isotherm. The EBA experiment successfully recovered 59.2% of the 503 antigen from the unclarified E. coli homogenate with a purification factor of 6.0pt_BR
dc.identifier.citationSOUSA JUNIOR, F. C.; VAZ, M. R. F.; PADILHA, C. E.; CHIBERIO, A. S.; MARTINS, D. R. A.; MACEDO, G. R.; SANTOS, E. S.. Recovery and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography. Journal of Chromatography. B (Print), p. 1, 2015. Disponivel em https://www.sciencedirect.com/science/article/abs/pii/S1570023215000719?via%3Dihub. Acesso em: 01 abr. 2021. https://doi.org/10.1016/j.jchromb.2015.01.031pt_BR
dc.identifier.doi10.1016/j.jchromb.2015.01.031
dc.identifier.issn1570-0232
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/32482
dc.languageenpt_BR
dc.publisherElsevierpt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectExpanded bed adsorptionpt_BR
dc.subjectLeishmania infantum chagasipt_BR
dc.subjectRecombinant protein purificationpt_BR
dc.subjectUnclarified bacterial homogenatept_BR
dc.subjectVisceral leishmaniasispt_BR
dc.titleRecovery and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatographypt_BR
dc.typearticlept_BR

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