Use of plasmids for expression of proteins from the genus Leishmania in Escherichia coli: current state and perspectives

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dc.contributor.authorSantos, Everaldo Silvino dos
dc.contributor.authorRibeiro, Vitor Troccoli
dc.contributor.authorLeitão, Ana Laura Oliveira de Sá
dc.contributor.authorVasconcelos, Luan Tales Costa de Paiva
dc.contributor.authorOliveira Filho, Marcos Antônio
dc.contributor.authorMartins, Daniella Regina Arantes
dc.contributor.authorSousa Júnior, Francisco Canindé de
dc.date.accessioned2021-05-14T14:41:40Z
dc.date.issued2020-03-25
dc.description.resumoLeishmaniosis is caused by the protozoa of the genus Leishmania with a wide spectrum of clinical and epidemiological manifestations which are characterized into four clinical groups: cutaneous, mucocutaneous, diffuse cutaneous, and visceral. American visceral leishmaniosis (AVL) or visceral leishmaniosis (VL) has been known as the most severe form of the disease. However, despite the growing number of people exposed to the infection risk and the great effort done by the scientific community worldwide to significantly increase the knowledge about these diseases, there is no vaccine capable of preventing VL in humans. In this short review, we present some of the plasmids used for the expression of recombinant protein by Escherichia coli strains used mainly for the second generation of vaccines for leishmaniosis. It can be emphasized that currently, these vectors and hosts play an important role in developing vaccine strategies against the disease. Indeed, use of the E. coli BL21 (DE) strain is remarkable mainly due to its characteristics for being a stable protein producer as well as the use of histidine tags for antigen purificationpt_BR
dc.identifier.citationRIBEIRO, Vitor Troccoli; LEITÃO, Ana Laura Oliveira de Sá; VASCONCELOS, Luan Tales Costa de Paiva; OLIVEIRA FILHO, Marcos Antônio; MARTINS, Daniella Regina Arantes; SOUSA JÚNIOR, Francisco Canindé de; SANTOS, Everaldo Silvino dos. Use of plasmids for expression of proteins from the genus Leishmania in Escherichia coli: current state and perspectives. Applied Microbiology and Biotechnology, [S.L.], v. 104, n. 10, p. 4273-4280, 25 mar. 2020. Disponível em: https://link.springer.com/article/10.1007%2Fs00253-020-10548-5. Acesso em: 06 abr. 2021. http://dx.doi.org/10.1007/s00253-020-10548-5.pt_BR
dc.identifier.doi10.1016/j.pep.2017.11.005
dc.identifier.issn0175-7598
dc.identifier.issn1432-0614
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/32527
dc.languageenpt_BR
dc.publisherSpringer-Verlag GmbH Germanypt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectLeishmaniosispt_BR
dc.subjectE. colipt_BR
dc.subjectRecombinant proteinpt_BR
dc.subjectAntigenpt_BR
dc.subjectPlasmidpt_BR
dc.titleUse of plasmids for expression of proteins from the genus Leishmania in Escherichia coli: current state and perspectivespt_BR
dc.typearticlept_BR

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