Coexpression of p53 protein and MDR functional phenotype in leukemias: the predominant association in chronic myeloid leukemia
| dc.contributor.author | Cavalcanti Junior, Geraldo Barroso | |
| dc.contributor.author | Vasconcelos, Flavia da Cunha | |
| dc.contributor.author | Faria, Giselle Pinto de | |
| dc.contributor.author | Scheiner, Marcos Antônio Maurício | |
| dc.contributor.author | Dobbin, Jane de Almeida | |
| dc.contributor.author | Klumb, Claudete Esteves | |
| dc.contributor.author | Maia, Raquel C. | |
| dc.contributor.authorID | https://orcid.org/0000-0001-9227-4145 | pt_BR |
| dc.date.accessioned | 2023-01-20T20:11:16Z | |
| dc.date.available | 2023-01-20T20:11:16Z | |
| dc.date.issued | 2004 | |
| dc.description.resumo | Background: One of the best characterized resistance mechanisms of leukemias is multidrug resistance (MDR) mediated by P-glycoprotein (Pgp) and multidrug-resistant related protein (MRP). In addition to Pgp and MRP, p53 mutation or inactivation might play a relevant role in therapeutic failure. Some studies have demonstrated that Pgp and MRP may be activated in association with overexpression of mutant or inactivated p53 protein. The aim of this study was to investigate the association between p53 expression and MDR functional phenotype analyzed by flow cytometry (FCM). Methods: Rhodamine-123 assay analyzed by FCM was used to detect the MDR phenotype that was positive in 18 out of 41 (43.9%) cases of chronic myeloid leukemia (CML), 16 out of 28 (57.1%) chronic lymphoid leukemia (CLL) cases, 11 out of 28 (39.3%) acute myeloid leukemia (AML) cases, and four out of 22 (18.2%) acute lymphoid leukemia (ALL) cases. Results: Variable levels of p53 expression were observed in leukemic cells: 12 out of 41 (29.2%) in CML, nine out of 28 (32.1%) in CLL, 15 out of 28 (53.6%) in AML, and eight out of 22 (36.4%) in ALL samples. Conclusions: In our study, no significant association between p53 expression and MDR functional phenotype was observed in ALL, CLL, and AML. On the other hand, a significant association (P 0.0003) of the coexpression was observed in CML. The p53 overexpression was more frequently seen in the accelerated phase and the blastic phase of this disease. Our results suggest that an MDR functional phenotype could be associated with p53 mutation in the advanced stage of leukemias. | pt_BR |
| dc.identifier.citation | CAVALCANTI JÚNIOR, Geraldo Barroso et al. Coexpression of p53 protein and MDR functional phenotype in leukemias: the predominant association in chronic myeloid leukemia. Cytometry Part B: Clinical Cytometry: The Journal of the International Society for Analytical Cytology, v. 61, n. 1, p. 1-8, 2004. Disponível em: https://onlinelibrary.wiley.com/doi/full/10.1002/cyto.b.20013. Acesso em: 20 jan. 2023. | pt_BR |
| dc.identifier.issn | 1552-4957 | |
| dc.identifier.uri | https://repositorio.ufrn.br/handle/123456789/51006 | |
| dc.language | en | pt_BR |
| dc.publisher | Wiley | pt_BR |
| dc.rights | Attribution-ShareAlike 3.0 Brazil | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-sa/3.0/br/ | * |
| dc.subject | Chronic myeloid leukemia | pt_BR |
| dc.subject | Acute myeloid leukemia | pt_BR |
| dc.subject | Multidrug resistance | pt_BR |
| dc.subject | P53 protein | pt_BR |
| dc.title | Coexpression of p53 protein and MDR functional phenotype in leukemias: the predominant association in chronic myeloid leukemia | pt_BR |
| dc.type | article | pt_BR |
Arquivos
Pacote Original
1 - 1 de 1
Nenhuma Miniatura disponível
- Nome:
- CoexpressionFunctionalPhenotype_CalvalcantiJunior_2013.pdf
- Tamanho:
- 293.42 KB
- Formato:
- Adobe Portable Document Format
Nenhuma Miniatura disponível
Licença do Pacote
1 - 1 de 1
Nenhuma Miniatura disponível
- Nome:
- license.txt
- Tamanho:
- 1.45 KB
- Formato:
- Item-specific license agreed upon to submission
Nenhuma Miniatura disponível