Expressão de dupla coloração de p16/ki-67 em citologia líquida em amostras de câncer cervical: uma revisão de escopo

Abstract

This review aims to present an overview of p16/Ki-67 dual-staining (DS) expression in Liquid Based Cytology (LBC) in samples from patients with cervical cancer (CC). Material and Methods: This scoping review was developed following the guidelines for systematic reviews and meta-analysesfor scoping reviews (PRISMA-ScR). A literature search using PubMed, Lilacs, Web of Science and Scopus electronic databases was conducted to identify studies that used DC for expression of p16/Ki-67 in CC in immunocytochemistry in LCB. The process of selecting and extracting data in a paired and independent way. The quality of included studies was assessed using the Joanna Briggs Institute Levels of Evidence. The review was registered in the Open Science Framework (OSF): DOI 10.17605/OSF.IO/9MUBQ. Results: Of the 149 studies analyzed, 7 studies, published between January 2018 and May 2023, were included in the qualitative synthesis. As expression parameters, p16/Ki-67 has been reported for DS in CBL in CC. Furthermore, we found that p16/Ki-67 in the CBL sample were expressed in squamous cell carcinoma(SCC). Conclusions: The study suggested that DC p16/Ki-67 is more frequently related to the CC group when compared to the HSIL and NILM group.