Recovery and purification of chitosanaseproduced by Bacillus cereus using expandedbed adsorption and central composite design
| dc.contributor.author | Santos, Everaldo Silvino dos | |
| dc.contributor.author | Macedo, Gorete Ribeiro de | |
| dc.contributor.author | Araújo, Nathália Kelly de | |
| dc.contributor.author | Pimentel, Vanessa Carvalho | |
| dc.contributor.author | Silva, Nayane Macedo Portela da | |
| dc.contributor.author | Padilha, Carlos Eduardo de Araújo | |
| dc.date.accessioned | 2021-04-26T15:01:00Z | |
| dc.date.available | 2021-04-26T15:01:00Z | |
| dc.date.issued | 2016-02 | |
| dc.description.resumo | This study presents a system for expanded bed adsorption for the purification of chitosanasefrom broth extract in a single step. A chitosanase-producing strain was isolated and identifiedas Bacillus cereus C-01 and used to produce chitosanases. The expanded bed adsorptionconditions for chitosanase purification were optimized statistically using STREAMLINETMDEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were defined by thequality criteria purification factor (P) and enzyme yield to optimize the chromatographicprocess. Statistical analyses showed that the optimum conditions for the maximum P were150 cm/h load flow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height.Distributor height had a strong influence on the process, considerably affecting both theP and enzyme yield. Optimizing the purification variables resulted in an approximately3.66-fold increase in the P compared with the value under nonoptimized conditions. Thissystem is promising for the recovery of chitosanase from B. cereus C-01 and is economicallyviable because it promotes the reduction steps | pt_BR |
| dc.identifier.citation | ARAÚJO, N.K.; PIMENTEL, V.C.; SILVA, N.M.P.; PADILHA, C.E.A.; MACEDO, G.R.; SANTOS, E.S.. Recovery and purification of chitosanase produced by using expanded bed adsorption and central composite design. Journal of Separation Science (Print), v. 39, p. 709-716, 2016. Disponivel em https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jssc.201500900. Acesso em 25 mar. 2021. https://doi.org/10.1002/jssc.201500900 | pt_BR |
| dc.identifier.doi | 10.1002/jssc.201500900 | |
| dc.identifier.issn | 1615-9306 | |
| dc.identifier.issn | 1615-9314 | |
| dc.identifier.uri | https://repositorio.ufrn.br/handle/123456789/32319 | |
| dc.language | en | pt_BR |
| dc.publisher | Wiley | pt_BR |
| dc.rights | Attribution 3.0 Brazil | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/br/ | * |
| dc.subject | Bioseparation | pt_BR |
| dc.subject | Chitosanase | pt_BR |
| dc.subject | Downstream Processing | pt_BR |
| dc.subject | Expanded bedadsorption | pt_BR |
| dc.title | Recovery and purification of chitosanaseproduced by Bacillus cereus using expandedbed adsorption and central composite design | pt_BR |
| dc.type | article | pt_BR |
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