Chronic brain functional ultrasound imaging in freely moving rodents performing cognitive tasks

dc.contributor.authorHady, Ahmed El
dc.contributor.authorTakahashi, Daniel Yasumasa
dc.contributor.authorSun, Ruolan
dc.contributor.authorAkinwale, Oluwateniola
dc.contributor.authorBoyd-Meredith, Tyler
dc.contributor.authorZhang, Yisi
dc.contributor.authorCharles, Adam S.
dc.contributor.authorBrody, Carlos D.
dc.date.accessioned2023-12-26T16:11:20Z
dc.date.available2023-12-26T16:11:20Z
dc.date.issued2023-12
dc.description.resumoBackground: Functional ultrasound imaging (fUS) is an emerging imaging technique that indirectly measures neural activity via changes in blood volume. Chronic fUS imaging during cognitive tasks in freely moving animals faces multiple exceptional challenges: performing large durable craniotomies with chronic implants, designing behavioural experiments matching the hemodynamic timescale, stabilizing the ultrasound probe during freely moving behavior, accurately assessing motion artifacts, and validating that the animal can perform cognitive tasks while tethered. New method: We provide validated solutions for those technical challenges. In addition, we present standardized step-by-step reproducible protocols, procedures, and data processing pipelines. Finally, we present proof-of-concept analysis of brain dynamics during a decision making task. Results: We obtain stable recordings from which we can robustly decode task variables from fUS data over multiple months. Moreover, we find that brain wide imaging through hemodynamic response is nonlinearly related to cognitive variables, such as task difficulty, as compared to sensory responses previously explored. Comparison with existing methods: Computational pipelines in fUS are nascent and we present an initial development of a full processing pathway to correct and segment fUS data. Conclusions: Our methods provide stable imaging and analysis of behavior with fUS that will enable new experimental paradigms in understanding brain-wide dynamics in naturalistic behaviorspt_BR
dc.identifier.citationHADY, Ahmed El; TAKAHASHI, Daniel; SUN, Ruolan; AKINWALE, Oluwateniola; BOYD-MEREDITH, Tyler; ZHANG, Yisi; CHARLES, Adam S.; BRODY, Carlos D. Chronic brain functional ultrasound imaging in freely moving rodents performing cognitive tasks. Journal of Neuroscience Methods, [S. l.], p. 110033, dez. 2023. Doi: http://dx.doi.org/10.1016/j.jneumeth.2023.110033. Disponível em: https://www.sciencedirect.com/science/article/pii/S0165027023002522?via%3Dihub. Acesso em: 26 dez. 2023pt_BR
dc.identifier.doi10.1016/j.jneumeth.2023.110033
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/56921
dc.languageenpt_BR
dc.publisherElsevier BVpt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectDecision making - Freely moving rodentspt_BR
dc.subjectExperimental design - Brain-wide dynamics in naturalistic behaviorspt_BR
dc.subjectFunctional ultrasound imagingpt_BR
dc.subjectImage processing - Cognitive taskspt_BR
dc.subjectMotion correction - Data processingpt_BR
dc.titleChronic brain functional ultrasound imaging in freely moving rodents performing cognitive taskspt_BR
dc.typearticlept_BR

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