Role of dopaminergic D2 receptors in regulating molecular biomarkers os neural lasticity and memory consolidation

dc.contributor.authorAguiar, L. M.
dc.contributor.authorSoares, B. L.
dc.contributor.authorFrança, A. S. C.
dc.contributor.authorNascimento, G. C. D.
dc.contributor.authorRibeiro, Sidarta Tollendal Gomes
dc.date.accessioned2017-11-27T16:29:50Z
dc.date.available2017-11-27T16:29:50Z
dc.date.issued2011-08
dc.description.resumoObjectives: To investigate whether the D2 antagonist haloperidol can modulate the hippocampal expression levels of Zif-268 and phosphorylated CaMKII, two synaptic plasticity biomarkers related to memory consolidation. Methods and Results: For memory consolidation analysis, male adult mice (Mus musculus, 19-29g) separated in two groups (haloperidol 0.3 mg/kg - HALO i.p. and vehicle, n=8 per group) were submitted to two sessions of object exploration with a 24 hours inter-session interval. Drugs were injected i.p. immediately after the first exploration session. An object recognition index (exploration time of unfamiliar/ familiar objects) was obtained in the second object exploration session. The HALO group presented a significant decrease in object recognition when compared to vehicle (respectively 1.51 ± 0.11 and 2.5 ± 0.19; p=0.0007). In a separate group of animals (n=10 for HALO and 11 for vehicle), the brains were removed three hours after a single session of object exploration, and the tissue was processed for immunohistochemistry of Zif-268 and phosphorilated CaMKII. Zif-268 expression was quantified by counts of positively-labeled nuclei, and CamKII phosphorylation was quantified by densitometry, with measurements taken from the hippocampal regions CA1, CA3 and dentate gyrus. We performed student t tests for comparisons between the two groups, and the significance level was set at 0.05. No significant difference related to Zif-268 expression was observed. On the other hand, a significant reduction in CaMKII phosphorylation in the CA1 region was observed in the HALO group, when compared to the vehicle group (0.84 ± 0.05 and 1.14 ± 0.1; p=0.016). Conclusions: These results suggest that the blockade of D2 receptors can impair mnemonic systems, leading to both a decrease in the phosphorylation of CAMKII, and to behavioral changes that indicate learning impairment.pt_BR
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/24381
dc.languageengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectDopaminept_BR
dc.subjectMemory consolidationpt_BR
dc.subjectSynaptic plasticitypt_BR
dc.subjectSleeppt_BR
dc.titleRole of dopaminergic D2 receptors in regulating molecular biomarkers os neural lasticity and memory consolidationpt_BR
dc.typeconferenceObjectpt_BR

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