Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/jspui/handle/1/3089
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dc.contributor.authorGomes, Carlos E. M.-
dc.contributor.authorBarbosa, Aulus E. A. D.-
dc.contributor.authorMacedo, Leonardo L. P.-
dc.contributor.authorPitanga, Joelma C. M.-
dc.contributor.authorMoura, Fabiano T.-
dc.contributor.authorOliveira, Adeliana S.-
dc.contributor.authorMoura, Raniere M.-
dc.contributor.authorQueiroz, Alexandre F. S.-
dc.contributor.authorMacedo, Francisco P.-
dc.contributor.authorAndrade, Lúcia B. S.-
dc.contributor.authorVidal, Márcia S.-
dc.contributor.authorSales, Mauricio P.-
dc.date.accessioned2010-09-30T12:26:54Z-
dc.date.available2010-09-30T12:26:54Z-
dc.date.issued2005-12-07-
dc.identifier.citationGOMES, C. E. M.; BARBOSA, A. E. A. D.; MACEDO, L. L. P.; PITANGA, J. C. M.; MOURA, F. T.; OLIVEIRA, A. S.; MOURA, R. M.; QUEIROZ, A. F. S.; MACEDO, F. P.; ANDRADE, L. B. S.; VIDAL, M. S.; SALES, M. P. (2005)pt_BR
dc.identifier.issn0981-9428-
dc.identifier.urihttp://repositorio.ufrn.br:8080/jspui/handle/1/3089-
dc.descriptionGOMES, Carlos E. M. et al. Effect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly). Plant Physiology and Biochemistry (Paris), v. 43, n. 12, p. 1095-1102, 2005.ISSN 0981-9428. DOI:10.1016/j.plaphy.2005.11.004.pt_BR
dc.description.abstractA proteinaceous trypsin inhibitor was purified from Crotalaria pallida seeds by ammonium sulfate precipitation, affinity chromatography on immobilized trypsin–Sepharose and TCA precipitation. The trypsin inhibitor, named CpaTI, had Mr of 32.5 kDa as determined by SDS-PAGE and was composed of two subunits with 27.7 and 5.6 kDa linked by disulfide bridges. CpaTI was stable at 50 °C and lost 40% of activity at 100 °C. CpaTI was also stable from pH 2 to 12 at 37 °C. CpaTI weakly inhibited chymotrypsin and lastase and its inhibition of papain, a cysteine proteinase, were indicative of its bi-functionality. CpaTI inhibited, in different degrees, digestive enzymes from Spodoptera frugiperda, Alabama argillacea, Plodia interpunctella, Anthonomus grandis and Zabrotes subfasciatus guts. In vitro and in vivo susceptibility of Calloso-bruchus maculatus and Ceratitis capitata to CpaTI was evaluated. C. maculatus and C. capitata enzymes were strongly susceptible, 74.4± 15.8% and 100.0 ± 7.3%, respectively, to CpaTI. When CpaTI was added to artificial diets and offered to both insect larvae, the results showed that C. maculatus was more susceptible to CpaTI with an LD50 of 3.0 and ED50 of 2.17%. C. capitata larvae were more resistant to CpaTI, in disagreement with the in vitro effects. The larvae were more affected at lower concentrations, causing 27% mortality and 44.4% mass decrease. The action was constant at 2–4% (w/w) with 15% mortality and 38% mass decrease.pt_BR
dc.language.isoporpt_BR
dc.publisherPlant Physiology and Biochemistrypt_BR
dc.rightsAcesso Aberto-
dc.subjectTrypsin inhibitorpt_BR
dc.subjectCrotalaria pallidapt_BR
dc.subjectCeratitis capitatapt_BR
dc.subjectCallosobruchus maculatuspt_BR
dc.subjectResistancept_BR
dc.titleEffect of trypsin inhibitor from Crotalaria pallida seeds on Callosobruchus maculatus (cowpea weevil) and Ceratitis capitata (fruit fly)pt_BR
dc.typearticlept_BR
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