Use este identificador para citar ou linkar para este item: https://repositorio.ufrn.br/jspui/handle/1/6299
Título: Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model
Título(s) alternativo(s): Influência da laparoscopia e laparotomia na gasometria, leucócitos e citocinas em modelo de sepse abdominal em ratos
Autor(es): Araújo Filho, Irami
Honorato Sobrinho, Abraão Allen
Rego, Amália Cinthia Meneses do
Garcia, Ana Claudia M. de Amorim
Fernandes, Daniele Pimentel
Cruz, Thaís Medeiros
Costa, Ticiana Cabral da
Medeiros, Aldo Cunha
Palavras-chave: Pneumoperitoneum;Carbon Dioxide;Sepsis;Acidosis;Leucocytes
Data do documento: 2006
Citação: ARAÚJO FILHO, Irami ; HONORATO SOBRINHO, Abraão Allen ; REGO, Amália Cinthia Meneses do ; GARCIA, Ana Claudia M. de Amorim ; FERNANDES, Daniele Pimentel ; CRUZ, Thaís Medeiros ; COSTA, Ticiana Cabral da ; MEDEIROS, Aldo Cunha. Influence of laparoscopy and laparotomy on gasometry, leukocytes and cytokines in a rat abdominal sepsis model. Acta Cirúrgica Brasileira , Brasil, v. 21, n.2, p. 74-79, 2006. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006000200004> Acesso em: 20 set. 2013.
Resumo: Laparoscopic surgery is associated with reduced surgical trauma, and less acute phase response, as compared with open surgery. Cytokines are important regulators of the biological response to surgical and anesthetic stress. The aim of this study was to determine if CO2 pneumoperitoneum would change cytokine expression, gas parameters and leukocyte count in septic rats. Methods: Wistar rats were randomly assigned to five groups: control (anesthesia only), laparotomy, CO2 pneumoperitoneum, cecum ligation and puncture by laparotomy, and laparoscopic cecum ligation and puncture. After 30 min of the procedures, arterial blood samples were obtained to determine leukocytes subpopulations by hemocytometer. TNFα, IL-1β, IL-6 were determined in intraperitoneal fluid (by ELISA). Gas parameters were measured on arterial blood, intraperitoneal and subperitoneal exsudates. Results: Peritoneal TNFα, IL-1β and IL-6 concentrations were lower in pneumoperitoneum rats than in all other groups (p<0.05). TNFα, IL-1β and IL-6 expression was lower in the laparoscopic than in laparotomic sepsis (p<0.05). Rats from laparoscopic cecum ligation and puncture group developed significant hypercarbic acidosis in blood and subperitoneal fluid when compared to open procedure group. Total white blood cells and lymphocytes were significantly lower in laparoscopic cecum ligation and puncture rats than in the laparotomic (p<0.01). Nevertheless, the laparotomic cecum ligation rats had a significant increase in blood neutrophils and eosinophils when compared with controls (p<0.05). Conclusions: This study demonstrates that the CO2 pneumoperitoneum reduced the inflammatory response in an animal model of peritonitis with respect to intraperitoneal cytokines, white blood cell count and clinical correlates of sepsis. The pneumoperitoneum produced hypercarbic acidosis in septic animals
URI: http://repositorio.ufrn.br:8080/jspui/handle/1/6299
ISSN: 1678-2674
Aparece nas coleções:CCS - DCIRUR - Artigos publicados em periódicos

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