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Title: Susceptibilities of enterococcus faecalis biofilms to some antimicrobial medications
Authors: Lima, Kenio Costa de
Fava, Luis R. G.
Siqueira Junior, José F.
Keywords: Biofilms;Enterococcus faecalis;Anti-Infective Agents
Issue Date: 2001
Citation: LIMA, K. C.; FAVA, L. F.; SIQUEIRA JUNIOR, J. F. Susceptibilities of Enterococcus faecalis biofilms to some antimicrobial medications. Journal of Endodontics, Dinamarca, v. 27, n. 10, p. 616-619, 2001.
Portuguese Abstract: Enterococcus faecalis has bEnterococcus faecalis has been suggested to be an important etiological agent in endodontic failures. The purpose of this study was to evaluate the effectiveness of chlorhexidine- or antibiotics-based medications in eliminating E. faecalis biofilms. One-day and three-day biofilms of E. faecalis were induced on cellulose nitrate membrane filters. Each biofilm-containing membrane was thoroughly covered with 1 ml of the test medications and incubated for 1 day at 37°C. Treated biofilms were then aseptically transferred to vials containing a neutralizing agent in saline solution and vortexed. Suspensions were 10-fold diluted, seeded onto Mitis salivarius agar plates, and the colony-forming units counted after 48 h of incubation. There were significant differences between the formulations tested. The association of clindamycin with metronidazole significantly reduced the number of cells in 1-day biofilms. However of all medications tested, only 2% chlorhexidine-containing medications were able to thoroughly eliminate most of both 1-day and 3-day E. faecalis biofilms.
Appears in Collections:CCS - DOD - Artigos publicados em periódicos

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