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Title: The growth of glioblastoma orthotopic xenografts in nude mice is directly correlated with impaired object recognition memory
Authors: Ana PaulaWasilewska-Sampaio
Tiago G. Santos
Marilene Hohmuth Lopes
Martin Cammarota
Vilma Regina Martins
Keywords: Glioblastoma;Nude mice;Xenografts;Tumor growth;Behavior
Issue Date: 2013
Portuguese Abstract: Cognitive dysfunction is found in patients with brain tumors and there is a need to determine whether it can be replicated in an experimental model. In the present study, the object recognition (OR) paradigm was used to investigate cognitive performance in nude mice, which represent one of the most important animal models available to study human tumors in vivo. Micewith orthotopic xenografts of the human U87MGglioblastoma cell line were trained at 9, 14, and 18 days (D9, D14, and D18, respectively) after implantation of 5×105 cells. At D9, the mice showed normal behaviorwhen tested 90min or 24h after training and compared to control nude mice. Animals at D14 were still able to discriminate between familiar and novel objects, but exhibited a lower performance than animals at D9. Total impairment in the OR memory was observed when animals were evaluated on D18. These alterations were detected earlier than any other clinical symptoms, which were observed only 22–24 days after tumor implantation. There was a significant correlation between the discrimination index (d2) and time after tumor implantation as well as between d2 and tumor volume. These data indicate that the OR task is a robust test to identify early behavior alterations caused by glioblastoma in nude mice. In addition, these results suggest that OR task can be a reliable tool to test the efficacy of new therapies against these tumors
ISSN: 0031-9384
Appears in Collections:ICe - Artigos publicados em periódicos

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