Use este identificador para citar ou linkar para este item: https://repositorio.ufrn.br/jspui/handle/123456789/23434
Título: Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes
Autor(es): Chouchane, Malek
Faria, Ana Raquel Melo de
Moura, Daniela Maria de Souza
Hilscher, Markus Michael
Schroder, Timm
Leão, Richardson
Costa, Marcos Romualdo
Palavras-chave: Lineage reprogramming;Induced neurons;Proneural genes;Astroglial cells;Cerebral cortex;Cerebellum;Cell transplantation
Data do documento: Jul-2017
Resumo: Astroglial cells isolated from the rodent postnatal cerebral cortex are particularly susceptible to lineage reprogramming into neurons. However, it remains unknown whether other astroglial populations retain the same potential. Likewise, little is known about the fate of induced neurons (iNs) in vivo. In this study we addressed these questions using two different astroglial populations isolated from the postnatal brain reprogrammed either with Neurogenin-2 (Neurog2) or Achaete scute homolog-1 (Ascl1). We show that cerebellum (CerebAstro) and cerebral cortex astroglia (CtxAstro) generates iNs with distinctive neurochemical and morphological properties. Both astroglial populations contribute iNs to the olfactory bulb following transplantation in the postnatal and adult mouse subventricular zone. However, only CtxAstro transfected with Neurog2 differentiate into pyramidal-like iNs after transplantation in the postnatal cerebral cortex. Altogether, our data indicate that the origin of the astroglial population and transcription factors used for reprogramming, as well as the region of integration, affect the fate of iNs.
URI: https://repositorio.ufrn.br/jspui/handle/123456789/23434
ISSN: 2213-6711
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