Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/jspui/handle/123456789/28858
Title: Effect of hydration with Ringer's solution submitted to magnetic field in an experimental model of hepatic ischemia-reperfusion injury in rats
Authors: Carriço, Artur da Silva
Azevedo, Ítalo Medeiros de
Freire, Juliana Mendonça
Souza, Jeovana Pinheiro Fernandes
Azevedo, Fabio Medeiros de
Medeiros, Aldo Cunha
Keywords: Ischemia;Reperfusion;Liver;Magnetics;Fluid therapy
Issue Date: 9-Nov-2018
Publisher: JOURNAL OF SURGICAL AND CLINICAL RESEARCH
Citation: CARRIÇO, Artur da Silva et al. Effect of hydration with Ringer's solution submitted to magnetic field in an experimental model of hepatic ischemia-reperfusion injury in rats. Journal of surgical and clinical research, v. 9, p. 44-53, 2018. ISSN 2179-7889 versão online. DOI https://doi.org/10.20398/jscr.v9i2.15916. Disponivel em https://periodicos.ufrn.br/jscr/article/view/15916. Acesso em : 30 abr. 2020.
Portuguese Abstract: Purpose:To evaluate the effect of hydration with Ringer's solution submitted to magnetic field in experimental model of hepatic ischemia/reperfusion in rats. Methods:Wistar rats weighing 389.58±46.88g were randomly allocated into 2 groups: control group (n = 6) -Hepatic ischemia/reperfusion and hydration with Ringer's solution; and test group (n = 6) -Hepatic ischemia/reperfusion and hydration with Ringer submitted to magnetic field of 20 mT during 2 hours. After anesthesia with ketamine 70 mg/kg and xylazine 7mg/kg i.p., the animals were weighed and operated by aseptic technique. The ischemia of the median and left liver lobes was induced for 30 minutes using a vascular clip. Then, i.v. hydration with 3 ml of Ringer in the control group, and 3 ml of magnetizedRinger’s solution in the test group. After 24 hours, blood sample and fragment of the left liver lobe were collected for the determination of AST, ALT, ALP and albumin levels, and histopathological examination. Results:There was no significant differencein comparison of the rats weight loss, biochemical measurements and histopathological scores between the groups (p>0.05). Conclusion:The hydration with Ringer's solution submitted to magnetic field after hepatic ischemia/reperfusion in rats did not alterliver unction tests neither histopathological scores, comparing with the control group.
Abstract: Effect of hydration with Ringer's solution submitted to magnetic field in an experimental model of hepatic ischemia-reperfusion injury in ratsFreire JM, et alJ Surg Cl Res –Vol. 9(2) 2018:44-5345function tests neither histopathological scores, comparing with the control group.Keywords:Ischemia. Reperfusion. Liver. Magnetics. Fluid therapy.RESUMOObjetivo:Avaliar o efeito da hidratação com solução de Ringer submetido à ação de campo magnético em modelo experimental de isquemia e reperfusão hepática em ratos. Métodos:Ratos Wistar pesando 389,58±46,88g foram distribuídos em 2 grupos: grupo controle (n=6) -Isquemia/reperfusão hepática e hidratação com solução de Ringer; e grupo teste (n=6) -Isquemia/reperfusão hepática e hidratação com Ringer submetido a campo magnético de 20 mT por 2 horas. Após anestesia com cetamina 70 mg/kg de peso e xilazina 7 mg/Kg i.p., os animais foram pesados e operados com técnica asséptica. A isquemia dos lobos mediano e esquerdo do fígado foi realizada por 30 minutos, usando clipe vascular. Em seguida, hidratação i.v.com 3 ml de Ringer no grupo controle, e 3 ml de Ringer submetido a campo magnético no grupo teste. Após 24 horas, sangue total e biópsia do lobo esquerdo do fígado foram colhidos para dosagem de AST, ALT, fosfatase alcalina e albumina, e exame histopatológico respectivamente. Resultados: Não houve diferença significante entre os grupos na comparação da perda de peso, das dosagens bioquímicas e dos escores histopatológicos (p>0,05). Conclusão: A hidratação com solução de Ringer submetida à ação de campo magnético após isquemia/reperfusão hepática em ratos não alterou as provas de função hepática nem os escores histopatológicos, comparando-se com o grupo controle.
URI: https://repositorio.ufrn.br/jspui/handle/123456789/28858
ISSN: 2179-7889 (online)
Appears in Collections:CCET - DFTE - Artigos publicados em periódicos



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