Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/jspui/handle/123456789/29186
Title: Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports
Authors: Scherer, Robison P.
Dallago, Rogério L.
Penna, Fábio G.
Bertella, Francile
Oliveira, Débora de
Oliveira, Jose Vladimir de
Pergher, Sibele Berenice Castellã
Keywords: Porcine pancreatic lipase;Immobilization;KSF;Natural montmorillonite;Pillared montmorillonite
Issue Date: Oct-2012
Publisher: Elsevier
Citation: SCHERER, Robison P.; DALLAGO, Rogério L.; PENNA, Fábio G.; BERTELLA, Francile; OLIVEIRA, Débora de; OLIVEIRA, Jose Vladimir de; PERGHER, Sibele Berenice Castellã. Influence of process parameters on the immobilization of commercial porcine pancreatic lipase using three low-cost supports. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 290-294, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.003. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001004. Acesso em: 08 jun. 2020.
Portuguese Abstract: The use of lipases as biocatalysts has attracted much attention recently, but industrial application has been hindered mainly due to the high production cost, determined by the production yield and enzyme stability. Immobilization of biocatalysts in inert supports can ensure their use for several batches, making possible to build cost-effective processes. The objective of this work was to investigate the influence of immobilization time and enzyme to support mass ratio on the yield of immobilization and esterification activity of porcine pancreatic lipase. Higher immobilization yields (38.2%) were obtained for pillared montmorillonite, 120 min of immobilization and enzyme to support mass ratio of 2:0.5. The highest esterification activity (1403 U/g) was achieved using the montmorillonite, after 180 min of immobilization and enzyme to support ratio of 2:1. The characterization of the supports, free and immobilized enzyme on different supports made possible to elucidate the immobilization results through the knowledge of support features.
URI: https://repositorio.ufrn.br/jspui/handle/123456789/29186
ISSN: 1878-8181
Appears in Collections:IQ - Artigos publicados em periódicos

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