Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/jspui/handle/123456789/29187
Title: Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571
Authors: Coghetto, Chaline C.
Scherer, Robison P.
Silva, Marceli F.
Golunski, Simone
Pergher, Sibele Berenice Castellã
Oliveira, Débora de
Oliveira, J. Vladimir
Treichel, Helen
Keywords: Kluyveromyces marxianus NRRL Y-7571;Inulinase;Inorganic support;Immobilization
Issue Date: Oct-2012
Publisher: Elsevier
Citation: COGHETTO, Chaline C.; SCHERER, Robison P.; SILVA, Marceli F.; GOLUNSKI, Simone; PERGHER, Sibele Berenice Castellã; OLIVEIRA, Débora de; OLIVEIRA, J. Vladimir; TREICHEL, Helen. Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 284-289, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.005. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001028. Acesso em: 08 jun. 2020.
Portuguese Abstract: The objective of this study was to investigate the process of immobilization of inulinases using natural montmorillonite as inorganic support. The enzyme to buffer ratio of 3:10 and 10 min of immobilization led to the highest specific activity, 375.07 U/mg protein. The immobilized inulinase kept its activity after 1968 h under storage at low temperatures and after 456–1826 h at high temperatures. The pH value of 3.5 led to the highest specific activity. Km values of 1.46 and 0.38 mM, and vmax of 0.2487 and 0.2396 mol/L min, were obtained, respectively, for sucrose and inulin.
URI: https://repositorio.ufrn.br/jspui/handle/123456789/29187
ISSN: 1878-8181
Appears in Collections:IQ - Artigos publicados em periódicos

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