Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/handle/123456789/31191
Title: Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed
Authors: Chaves Filho, Gildácio Pereira
Sousa, Angélica Fernandes Gurgel de
Câmara, Rafael Barros Gomes da
Rocha, Hugo Alexandre de Oliveira
Medeiros, Silvia Regina Batistuzzo de
Moreira, Susana Margarida Gomes
Keywords: Bone regeneration;Osteoinductive effect;Green macroalgae
Issue Date: 2018
Publisher: Elsevier
Citation: CHAVES FILHO, Gildácio Pereira; SOUSA, Angélica Fernandes Gurgel de; CÂMARA, Rafael Barros Gomes da; ROCHA, Hugo Alexandre Oliveira; MEDEIROS, Silvia Regina Batistuzzo de; MOREIRA, Susana Margarida Gomes. Genotoxicity and osteogenic potential of sulfated polysaccharides from Caulerpa prolifera seaweed. International Journal Of Biological Macromolecules, [s. l.], v. 114, p. 565-571, jul. 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/pii/S0141813017345518?via%3Dihub. Acesso em: 03 ago. 2020. http://dx.doi.org/10.1016/j.ijbiomac.2018.03.132.
Portuguese Abstract: Marine algae are sources of novel bioactive molecules and present a great potential for biotechnological and biomedical applications. Although green algae are the least studied type of seaweed, several of their biological activities have already been described. Here, we investigated the osteogenic potential of Sulfated Polysaccharide (SP)- enriched samples extracted from the green seaweed Caulerpa prolifera on human mesenchymal stem cells isolated from Wharton jelly (hMSC-WJ). In addition, the potential genotoxicity of these SPs was determined by cytokinesis-block micronucleus (CBMN) assay. SP-enriched samples did not show significant cytotoxicity towards hMSCs-WJ at a concentration of up to 10 μg/mL, and after 72 h of exposure. SP enrichment also significantly increased alkaline phosphatase (ALP) activity, promoting calcium accumulation in the extracellular matrix. Among the SP-enriched samples, the CP0.5 subfraction (at 5 μg/mL) presented the most promising results. In this sample, ALP activity was increased approximately by 60%, and calcium accumulation was approximately 6-fold above the negative control, indicating high osteogenic potential. This subfraction also proved to be non-genotoxic, according to the CBMN assay, as it did not induce micronuclei. The results of this study highlight, for the first time, the potential of these SPs for the development of new therapies for bone regeneration
URI: https://repositorio.ufrn.br/handle/123456789/31191
ISSN: 0141-8130 (print)
Appears in Collections:CB - DBG - Artigos publicados em periódicos
CB - DBQ - Artigos publicados em periódicos
EMCM - Artigos publicados em periódicos

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