Farias, Daniel Solon Dias deTeixeira, Gabriel Dutra2024-08-092024-08-092024-04-05TEIXEIRA, Gabriel Dutra. Análise morfológica do trato gastrointestinal de bobo-pequeno (Puffinus puffinus) e suas causas de encalhe na costa da bacia Potiguar, Nordeste brasileiro. Orientador: Dr. Daniel Solon Dias de Farias. 2024. 65f. Dissertação (Mestrado em Biologia Estrutural e Funcional) - Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, 2024.https://repositorio.ufrn.br/handle/123456789/59110Puffinus puffinus is an animal species that belongs to a migratory seabird, and migrates to the South Atlantic crossing the Hemisfere when in the non-reproductive stage, to spend the summer. The coastal zone of the brazillian northeastern region is considered a relevant spot for the migratory routes of seabirds, in special, the region named Potiguar Basin (Rio Grande do Norte and Ceará). This area is monitored by an Beach Monitoring Project (in portuguese PMP-BP) that records, annually, a lot of specimens stranded alive or dead. The aim of this work is to determine the spatial-temporal pattern of stranded Puffinus puffinus in the States of RN and Ceará, and identify the most frequent causes of death and perform a histopathological diagnosis for this specimen. Were used data from the PMP-BP collected in daily monitoring since 2011 to 2022. The animals founded alive were forwarded to the Rehabilitation Center of Marine Fauna from Projeto Cetáceos da Costa Branca from Estadual University of Rio Grande do Norte in the city of Areia Branca (RN) and the dead animals were conducted to the Laboratory of Marine Biota for necropsy procedures. In these necropsies were collected tissue samples of organs that presented macroscopic alterations, in addition to gonads for sexing. These collected material underwent histological processing at the Vertebrate Morphology Laboratory at Federal University of Rio Grande do Norte. Sixty-six (66) individuals were recorded during the monitored period, 14 males, 17 females and 35 with undetermined sex. Of these, 43 individuals were classified as adults and 12 as juveniles. The spatio-temporal distribution analysis indicated that 33 animals stranded in the State of CE and 33 in the State of RN, from September to February. Regarding the analysis of cause of death, it was detected that marine debris, mechanical trauma and oil contamination are among the categories with an anthropogenic relationship and account for approximately 33% of the causes of death. The other causes were related to exhaustion, sepsis and parasitosis. Histological analysis of the digestive tract revealed the presence of the four tunics characteristic of the system: the mucosa, submucosa, muscular and adventitia/serosa layers. The esophagus presents with a very pleated mucosa region covered by nonkeratinized stratified squamous epithelium, followed by a lamina propria with several mucous glands. The muscularis mucosa leading to the submucosa is present. A welldeveloped muscular layer, with an inner circular layer and an outer longitudinal layer. Finally, there is the serosa layer, composed of loose connective tissue, thus allowing blood supply. The proventricle is the glandular portion of the stomach, so its mucous layer is characterized by being folded. The lining epithelium is simple columnar in the folds, while in the grooves formed by the depressions of the folds it is characterized as simple cuboidal. The submucosa is mainly made up of proventricular glands. The muscular and serosa remains standard. In the ventricle we observe villi that make up the lining epithelium. This lining surface is composed of simple columnar epithelium alternating to simple cuboidal. The muscularis mucosa is not identified. The submucosal layer is thin and aglandular. The muscular layer is more developed, but the pattern remains, along with the serous layer. In the small intestine, the mucous layer of the epithelium lining the villi and crypts is simple columnar epithelium with microvilli. The muscular layer of the mucosa is well developed. The submucosal layer is almost imperceptible. Finally, the muscular layer and the serosa layer will present the same configuration already evidenced by the other tissues. The interpretation and organization of necropsy reports combined with histological tissue analysis offer more complete answers within the field of conservation strategies in favor of these animals.Acesso AbertoHistologia gastrointestinalNecropsiaEncalhemasterThesisCNPQ::CIENCIAS BIOLOGICAS