Navegando por Autor "Pastrana, Lorenzo"
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Artigo Anti-Inflammatory protein isolated from tamarind promotes better histological aspects in the intestine regardless of the improvement of intestinal permeability in a preclinical study of diet-induced obesity(Nutrients, 2022) Morais, Ana Heloneida de Araújo; Lima, Mayara S. R.; Gonçalves, Catarina; D. Neto, Mafalda; Macedo, Maria Helena; Queiroz, Jaluza L. C. de; Silva, Valéria C. da; Costa, Izael de S.; Camillo, Christina da S.; Santos, Pedro Paulo de A.; Lima, Aldo A. M.; Pastrana, Lorenzo; Maciel, Bruna L. L.; https://orcid.org/0000-0002-6460-911XObesity is associated with metabolic and physiological effects in the gut. In this study, we evaluated the anti-inflammatory effect of trypsin inhibitor isolated from tamarind seeds (TTI) in vitro (interaction with lipopolysaccharide (LPS) and inhibitory activity against human neutrophil elastase (HNE)), and using intestinal co-cultures of Caco-2:HT29-MTX cell lines inflamed with TNF α (50 ng/mL) and a Wistar rat model of diet-induced obesity (n = 15). TTI was administered to animals by gavage (10 days), and the treated group (25 mg/kg/day) was compared to animals without treatment or treated with a nutritionally adequate diet. In the in vitro study, it showed inhibitory activity against HNE (93%). In co-cultures, there was no protection or recovery of the integrity of inflamed cell monolayers treated with TTI (1.0 mg/mL). In animals, TTI led to lower plasma concentrations of TNF-α and IL-6, total leukocytes, fasting glucose, and LDL-c (p < 0.05). The intestines demonstrated a lower degree of chronic enteritis, greater preservation of the submucosa, and greater intestinal wall thickness than the other groups (p = 0.042). Therefore, the better appearance of the intestine not reflected in the intestinal permeability added to the in vitro activity against HNE point to possibilities for new studies and applications related to this activity.Artigo Prospecting in silico antibacterial activity of a peptide from trypsin inhibitorisolated from tamarind seed(Journal of Enzyme Inhibition and Medicinal Chemistry, 2022-08-28) Passos, Thais Souza; Oliveira, Gerciane Silva de; Nascimento, Amanda Maria de Souza; Luz,Anna Beatriz Santana; Aguiar, Ana Júlia Felipe Camelo; Lima, Mayara Santa Rosa; Matias, Lídia Leonize Rodrigues; Amado, Isabel Rodríguez; Damasceno, Karla Suzane Florentino da Silva Chaves; Monteiro, Norberto de Kássio Vieira; Moreira, Susana Margarida Gomes; Pastrana, Lorenzo; Morais, Ana Heloneida de Araújo; https://orcid.org/0000-0003-2054-1544; https://orcid.org/0000-0002-5847-5733; https://orcid.org/0000-0002-6460-911XBacterial infections have become a global concern, stimulating the growing demand for natural and bio-logically safe therapeutic agents with antibacterial action. This study was evaluated the genotoxicity ofthe trypsin inhibitor isolated from tamarind seeds (TTI) and the antibacterial effect of TTI theoric model,number 56, and conformation number 287 (TTIp 56/287) and derived peptides in silico. TTI (0.3 and0.6 mg.mL 1 ) did not cause genotoxicity in cells (p > 0.05). In silico, a greater interaction of TTIp 56/287with the Gram-positive membrane (GP) was observed, with an interaction potential energy (IPE) of -1094.97 kcal.mol-1 . In the TTIp 56/287-GP interaction, the Arginine, Threonine (Thr), and Lysine residuespresented lower IPE. In molecular dynamics (MD), Peptidotrychyme59 (TVSQTPIDIPIGLPVR) showed an IPEof -518.08 kcal.mol-1 with the membrane of GP bacteria, and the Thr and Arginine residues showed thegreater IPE. The results highlight new perspectives on TTI and its derived peptides antibacterial activityArtigo Safety and potential functionality of nanoparticles loaded with a trypsin inhibitor isolated from tamarind seeds(Elsevier, 2020) Morais, Ana Heloneida Araújo; Costa, Rafael Oliveira de Araújo; Matias, Lídia Leonize Rodrigues; Passos, Thaís Souza; Queiroz, Jaluza Luana Carvalho de; Carvalho, Fabiana Maria Coimbra de; Maciel, Bruna Leal Lima; Uchôa, Adriana Ferreira; Amado, Isabel Rodríguez; Gonçalves, Catarina; Pastrana, Lorenzo; https://orcid.org/0000-0002-6460-911XThe trypsin inhibitor isolated from tamarind seeds(TTI) presentssatietogenic and anti-inflammatory effects. In the present study, TTI encapsulation by nanoprecipitation in purified chitosan and whey protein isolate (ECW) was performed to maintain TTI’s integrity and protection through the gastrointestinal tract. The interaction between TTI and the encapsulating agents was determined by filtration monitoring the antitrypsin activity. Cytotoxicity was evaluated at different concentrations (0.5, 2.5 and 5.0 mg/mL) in Caco-2 and CCD-18Co cells. Subacute blood toxicity was evaluated in Wistar rats (12.5 mg/kg) for ten days. The particles presented a smooth surface, with 118.00 (17.27) nm of diameter, 0.37 (0.02) of polydispersity index, -38.26 (0.15) mV (neutral pH) of sur face charge, and 95.31 (0.31)% of incorporation efficiency. At neutral pH, there was a strong interaction of the encapsulating agents and TTI, and a gradual release of TTI at pH 3.0. Cell viability (> 70%) evaluation, in vivo biochemical and hepatic parameters of Wistar rats showed the absence of toxic effects of the nanoparticles with TTI. The study showed that TTI nanoparticles are potentially safe and represent a promising formulation for the clinical application of TTI and may become an option as a novel ingredient in foodArtigo Tamarind trypsin inhibitor in chitosan–Whey protein nanoparticles reduces fasting blood glucose levels without compromising Insulinemia: a preclinical study(Nutrients, 2019) Morais, Ana Heloneida de Araújo; Matias, Lidia Leonize Rodrigues; Costa, Rafael Oliveira de Araújo; Passos, Thais Souza; Queiroz, Jaluza Luana Cravalho de; Serquiz, Alexandre Coelho; Maciel, Bruna Leal Lima; Santos, Pedro Paulo de Andrade; Camillo, Cristina da Silva; Gonçalves, Catarina; Amado, Izabel Rodriguez; Pastrana, Lorenzo; https://orcid.org/0000-0002-6460-911XIn vivo studies show the benefits of the trypsin inhibitor isolated from tamarind (Tamarindusindica L.) (TTI) seeds in satiety and obesity. In the present study, TTI nanoencapsulation (ECW) was performed to potentialize the effect of TTI and allow a controlled release in the stomach. The impact on glycemia, insulin, and lipid profile was evaluated in Wistar rats overfed with a high glycemic index diet (HGLI). Characterization of the nanoparticles and in vitro stability in simulated gastrointestinal conditions, monitored by antitrypsin activity and HPLC, was performed. ECW and empty nanoparticles (CW) were administered by gavage, using 12.5 and 10.0 mg/kg, respectively. Both nanoformulations presented a spherical shape and smooth surface, with an average diameter of 117.4 nm (24.1) for ECW and 123.9 nm (11.3) for CW. ECW maintained the antitrypsin activity (95.5%) in the gastric phase, while TTI was completely hydrolyzed. In Wistar rats, the nanoformulations significantly reduced glycemia and HOMA IR, and ECW increased HDL-c compared to CW (p < 0.05).Pancreas histopathology of animals treated with ECW suggested an onset of tissue repair. Thenanoencapsulation provided TTI protection, gradual release in the desired condition, and improvement of biochemical parameters related to carbohydrate metabolism disorders,without compromising insulinemia