CCS - DFAR - Artigos publicados em periódicos

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Evaluation of medicines advertising in medical journals
(Universidade de São Paulo (Faculdade de Ciências Farmacêuticas), 2009-06) Souza, Daliana Maria Berenice de Oliveira; Lima, Suellen Cristiane Medeiros de; Batista, Almária Mariz; Carvalho, Maria Cleide Ribeiro Dantas de
Este trabalho objetivou analisar propagandas de medicamentos sujeitos à prescrição, que foram veiculadas em três periódicos das áreas de cardiologia e neurologia, dirigidos a profissionais de saúde. A análise envolveu critérios baseados na legislação em vigor, além de outros, e das referências bibliográficas. Averiguou-se a presença dos seguintes itens: número de registro, nome do fármaco, indicações terapêuticas, contra-indicações, cuidados e advertências, reações adversas, efeitos colaterais, posologia, legibilidade das informações técnico-científicas e referências bibliográficas, frases e/ou expressões sobre vantagens ante outros fármacos, mensagens de segurança, promessas de cura e imagens de pessoas sorridentes, além da confirmação das citações com base nas referências bibliográficas. Dentre os critérios legais avaliados, observou-se a ausência de legibilidade das informações técnico-científicas em 85% das peças promocionais; a ausência dos efeitos colaterais em 23%; a ausência de cuidados e advertências em 15%; de contra-indicações em 12,8%; de posologia em 6,4%; do número de registro em 2,7% e da Denominação Comum Brasileira/Denominação Comum Internacional (DCB/DCI) em 0,6%. Das 130 afirmações relativas a benefícios frente a outros fármacos, 23,8% não foram confirmadas; e das 48 mensagens de segurança veiculadas, 41,7% não tinham respaldo nas referências citadas. As imagens de pessoas sorridentes foram recurso usado em 49,2 % das peças promocionais. Das 1.362 referências analisadas, 19,7% não foram encontradas e 37,1% das afirmações referenciadas não foram confirmadas
Analysis of advertisements of infant food commercialized in the city of Natal, Rio Grande do Norte, Brazil
(Universidade de São Paulo (Faculdade de Ciências Farmacêuticas), 2009-06) Pagnoncelli, Maria Giovana Binder; Batista, Almária Mariz; Silva, Michelle Cristine Medeiros da; Costa, Ana Paula Marques da; Araújo, Fábio Resende de; Marques, Marcela Pinheiro; Fidalgo, Cláudia Margareth de Quelhas; Carvalho, Maria Cleide Ribeiro Dantas de Carvalho
A propaganda de substitutos do leite materno tem sido apontada como um dos fatores responsáveis pelos baixos índices da amamentação. Neste sentido, dispositivos legais foram criados para assegurar o uso desses produtos, sem que haja interferência no aleitamento materno. Este estudo objetivou analisar propagandas de alimentos abrangidos pela Lei nº 11.265/2006, que regulamenta a comercialização e publicidade de alimentos para lactentes e crianças de primeira infância no Brasil, no período de junho 2006 a maio de 2008, na cidade de Natal/RN. Foram coletadas 220 propagandas, sendo 141 de material técnico-científico e 79 de promoção comercial que foram avaliadas quanto às exigências legais. O resultado desta análise demonstrou que 100% das propagandas veiculadas em material técnico-científico infringiram o inciso V; 18,4% o inciso IV e 14,2% os incisos I, II e III do artigo 19 da Lei nº 11.265/2006. Em 42% das promoções comerciais não foram veiculadas as informações obrigatórias constantes dos incisos I e II do artigo 5º. Em 8,7% das que continham essas informações, estas estavam postas em local que dificultava sua identificação e leitura devido ao tamanho e cor das letras, semelhantemente ao ocorrido com as informações exigidas pelo artigo 19 nas propagandas veiculadas em material técnico-científico
Chitooligosaccharides enzymatic production by Metarhizium anisopliae
(Springer Science and Business Media LLC, 2010-02-18) Santos, Everaldo Silvino dos; Assis, Cristiane Fernandes de; Araújo, Nathália Kelly; Pagnoncelli, Maria Giovana Binder; Pedrini, Márcia Regina da Silva; Macedo, Gorete Ribeiro de
The products of chitosan hydrolysis are chitooligosaccharides and are used mainly for medical applications due to their specific biological activities. The objective of this study was to detect and identify the products of enzymatic hydrolysis of chitosan (dimers to hexamers) using a crude extract of chitosanolytic enzymes produced by the fungus Metarhizium anisopliae. These fungus was able to produce, during 48 h cultivation in a medium containing chitosan, chitooligosaccharides ranging from dimers, trimers, tetramers and pentamers at concentrations 0.2, 0.19, 0.06, 0.04 mg/mL, respectively, and the enzymatic activity was 2.5 U/L. Using the crude enzyme extract for chitosan hydrolysis, we detected the presence of dimers to hexamers at hydrolysis times of 10, 20, 30, 40, 50 and 60 min of enzymatic reaction, but the yields were higher at 10 min (54%). The hexamers was obtained only with 30 min of reaction with concentration of 0.004 mg/mL
Influence of culture medium on the production of eif antigen from Leishmania chagasi in recombinant Escherichia coli
(Sociedade Brasileira de Microbiologia, 2011-12) Santos, Everaldo Silvino dos; Vaz, Michelle Rossana Ferreira; França, Ricardo Luiz Soares de; Andrade, Sirtys Santos Lessa de; Sousa Junior, Francisco Canindé de; Martins, Daniella Regina Arantes; Macedo, Gorete Ribeiro de
With the advent of recombinant DNA technology, recombinant protein expression has become an important tool in the study of the structure, function and identification of new proteins, especially those with therapeutic functions. Escherichia coli has been the predominant prokaryote used in genetic engineering studies due to the abundance of information about its metabolism. Despite significant advances in molecular biology and immunology of infections, there are as yet no prophylactic drugs capable of preventing visceral leishmaniasis. It is therefore important to identify specific antigens in order to develop vaccines and diagnostic kits against this disease. The objective of this study was to evaluate the influence of culture medium on the production of eIF antigen from Leishmania chagasi in recombinant Escherichia coli. An induction procedure using IPTG was carried out in a series of trials, to observe the influence of culture medium (2xTY, TB) under expression of the recombinant eIF protein. Results showed that recombinant protein expression was associated to growth and that the highest eIF antigen expression was obtained in the 2xTY medium
Cytotoxicity of chitosan oligomers produced by crude enzyme extract from the fungus metarhizium anisopliae in hepg2 and hela cells
(Hilaris Publisher, 2012) Santos, Everaldo Silvino dos; Assis, Cristiane Fernandes de; Melo-Silveira, Raniere Fagundes; Oliveira, Ruth Medeiros de; Costa, Leandro Silva; Rocha, Hugo Alexandre de Oliveira; Macedo, Gorete Ribeiro de
Chitooligosaccharides exhibit biological activities, including antitumor, antimicrobial and antioxidant. In this study we used a mixture of chitooligosaccharides produced by enzymatic hydrolysis in two tumor cell lines and assessed the cell proliferation and cytotoxicity of these compounds. The proliferation of HeLa cells was inhibited around by 60%
Chitooligosaccharides antagonize the cytotoxic effect of glucosamine
(Springer, 2012-10-02) Santos, Everaldo Silvino dos; Assis, Cristiane Fernandes de; Costa, Leandro Silva; Melo-Silveira, Raniere Fagundes; Oliveira, Ruth Medeiros; Pagnoncelli, Maria Giovana Binder; Rocha, Hugo Alexandre Oliveira; Macedo, Gorete Ribeiro de
Chitooligosaccharides (COS) are partially hydrolyzed compounds derived from chitosan that exhibit a number of biological activities, including antitumor, antibacterial and antifungal properties. In this work, we examined the cytotoxicity of pure COS and oligomers A, B and C (solutions composed of different amounts of COS) produced by enzymatic hydrolysis using a crude enzyme extract produced by the fungus Metarhrizium anisopliae. The antiproliferative effect of these molecules was analyzed using tumor cell lines (HepG2 and HeLa cells) and in a normal cell line (3T3). The antioxidant activity was analyzed in several in vitro experiments. Glucosamine showed higher toxicity (approximately 92%) to all cell lines studied. However, the oligomers obtained after hydrolysis demonstrated no toxic effects on the normal cells (3T3). Furthermore, we showed that a small amount of other COS can decrease the cytotoxic effect of glucosamine against 3T3 cells, indicating that glucosamine could be used as an antitumor drug in the presence of other COS. In addition, different effects were found in antiproliferative assays, which depended on the COS composition in the oligomers (A, B and C), showing that a combination of them may be essential for developing antineoplastic drugs. Superoxide anion scavenging was the main antioxidant activity demonstrated by the COS and oligomers. This activity was also dependent on the oligomer composition of the chitosan hydrolysates. Further work will identify the ideal proportions of COS and glucosamine for maximizing the effects of these biological activities
Avaliação da propaganda de medicamentos veiculada em emissoras de rádio
(ABRASCO - Associação Brasileira de Saúde Coletiva, 2013-02) Batista, Almária Mariz; Carvalho, Maria Cleide Ribeiro Dantas de
O objetivo deste estudo foi avaliar propagandas de medicamentos veiculadas em emissoras de rádio em Natal (RN), Brasil, nos períodos de abril a setembro de 2008 e abril a setembro de 2010. As propagandas foram captadas, gravadas e transcritas para se efetuar uma análise legal e uma de conteúdo baseada nos preceitos de Laurence Bardin. Tanto as captadas no primeiro período (sob égide da RDC 102/00), quanto às do segundo (sob égide da RDC 96/08), apresentaram algum tipo de infração legal. A análise de conteúdo detectou, em ambos os períodos, praticamente os mesmos vícios como ausência de informações negativas sobre o medicamento, apelo ao consumo, exaltação da eficiência/eficácia e exploração abusiva de enfermidades. Apesar da mudança para uma legislação mais moderna e mais restritiva, as propagandas de medicamentos, no rádio, continuam a infringir frontalmente a legislação, cometendo abusos e desrespeitando o direito à saúde da população. O estudo aponta para a necessidade do tema propaganda de medicamentos ser tratado em um contexto mais amplo, ou seja, discutido como questão de saúde pública, considerando-se o cenário histórico-social em que se desenvolveu, uma vez que só a legislação não é suficiente para coibir abusos praticados em detrimento da saúde pública
Production of enzymes by paenibacillus chitinolyticus and paenibacillus ehimensis to obtain chitooligosaccharides
(Springer, 2013-03-17) Santos, Everaldo Silvino dos; Araújo, Nathália Kelly de; Assis, Cristiane Fernandes de; Macedo, Gorete Ribeiro de; Farias, Louise Fonseca de; Arimatéia Júnior, Humberto; Pedrosa, Matheus de Freitas Fernandes; Pagnoncelli, Maria Giovana Binder
Obtaining oligosaccharides from chitosan has been the focus of several studies in the pharmaceutical, chemical, food, and medical areas, due to their functional properties. Here, we evaluated the production potential of biologically functional chitooligosaccharides using enzymes extracts produced by Paenibacillus chitinolyticus and Paenibacillus ehimensis. After 48 h of fermentation, these microorganisms were able to produce chitosanases, which generated oligomers with a degree of polymerization between dimers and hexamers. The maximum conversion of chitosan to oligomers was 99.2 %, achieved after 12 h incubation of chitosan with enzymes produced by P. ehimensis. The chitooligosaccharides generated were capable of scavenging the 2,2-diphenyl-1-picrylhydrazyl radical, reaching a maximum scavenging rate of 61 and 39 % when produced with P. ehimensis and P. chitinolyticus enzymes, respectively. The use of these enzymes in the crude form could facilitate their use in industrial applications
Efeitos da estratégia de indução na produção de antígeno 503 de Leishmania infatum chagasi em E. coli recombinante
(Universidade Federal de Campina Grande (UFCG), 2014) Santos, Everaldo Silvino dos; Sousa Júnior, Francisco Canindé de; Silva, Valéria Nogueira da; Vaz, Michelle Rossana Ferreira; Chibério, Abimaelle Silva; Dias, Emanuele Cardoso; Ribeiro, Vitor Troccoli; Macedo, Gorete Ribeiro de
O presente trabalho teve como objetivo estudar o efeito de duas estratégias de indução (pulso e adição gradual) com IPTG na expressão do antígeno 503 de Leishmania infatum chagasi em E. coli. No ensaio em batelada, o IPTG foi adicionado ao cultivo na concentração final de 1 mM. Na batelada alimentada, 0,2 mM do indutor foi adicionado a cada hora, totalizando uma concentração final de 0,8 mM. O procedimento de indução por pulsos de IPTG indicou um incremento de aproximadamente 49% na produção de biomassa quando comparado em batelada. Pode-se concluir que a estratégia de indução empregando pulsos de IPTG (batelada alimentada) foi mais eficaz na expressão do antígeno 503 quando comparado à estratégia de batelada simples. A eficácia da estratégia de indução foi confirmada por eletreforese
Aqueous leaf extract of Jatropha gossypiifolia L. (Euphorbiaceae) inhibits enzymatic and biological actions of Bothrops jararaca snake venom
(Public Library of Science, 2014-08-15) Félix-Silva, Juliana; Souza, Thiago; Menezes, Yamara A. S.; Cabral, Bárbara; Câmara, Rafael Barros Gomes da; Silva-Junior, Arnóbio Antonio da; Rocha, Hugo Alexandre de Oliveira; Rebecchi, Ivanise Marina Moretti; Langassner, Silvana Maria Zucolotto; Fernandes-Pedrosa, Matheus de Freitas
Snakebites are a serious public health problem due their high morbi-mortality. The main available specific treatment is the antivenom serum therapy, which has some disadvantages, such as poor neutralization of local effects, risk of immunological reactions, high cost and difficult access in some regions. In this context, the search for alternative therapies is relevant. Therefore, the aim of this study was to evaluate the antiophidic properties of Jatropha gossypiifolia, a medicinal plant used in folk medicine to treat snakebites. The aqueous leaf extract of the plant was prepared by decoction and phytochemical analysis revealed the presence of sugars, alkaloids, flavonoids, tannins, terpenes and/or steroids and proteins. The extract was able to inhibit enzymatic and biologic activities induced by Bothrops jararaca snake venom in vitro and in vivo. The blood incoagulability was efficiently inhibited by the extract by oral route. The hemorrhagic and edematogenic local effects were also inhibited, the former by up to 56% and the latter by 100%, in animals treated with extract by oral and intraperitoneal routes, respectively. The inhibition of myotoxic action of B. jararaca reached almost 100%. According to enzymatic tests performed, it is possible to suggest that the antiophidic activity may be due an inhibitory action upon snake venom metalloproteinases (SVMPs) and/or serine proteinases (SVSPs), including fibrinogenolytic enzymes, clotting factors activators and thrombin like enzymes (SVTLEs), as well upon catalytically inactive phospholipases A2 (Lys49 PLA2). Antiinflammatory activity, at least partially, could also be related to the inhibition of local effects. Additionally, protein precipitating and antioxidant activities may also be important features contributing to the activity presented. In conclusion, the results demonstrate the potential antiophidic activity of J. gossypiifolia extract, including its significant action upon local effects, suggesting that it may be used as a new source of bioactive molecules against bothropic venom
Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography
(BMC, 2014-10-01) Santos, Everaldo Silvino dos; Sousa Junior, Francisco Canindé de; Vaz, Michelle Rossana Ferreira; Padilha, Carlos Eduardo de Araújo; Martins, Daniella Regina Arantes; Macedo, Gorete Ribeiro de
Visceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Some authors have reported that as much as 50% of overall costs in the biotechnology industries are related to downstream processing. Thus, the development of new and economically advantageous purification methods is a challenge [2]. Expanded bed adsorption (EBA) is an innovative chromatography technology that allows the adsorption of target proteins directly from unclarified feedstock. EBA technology combines solid-liquid separation with an adsorption step in a single-unit operation, aiming at increased overall yield, reduced operational time, and less capital investment and consumables [3,4]. Thus, the aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from crude feedstock using EBA chromatography
Production of recombinant 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch
(BMC Proceedings, 2014-10-10) Santos, Everaldo Silvino dos; Vaz, Michelle Rossana Ferreira; Sousa Junior, Francisco Canindé de; Padilha, Carlos Eduardo de Araújo; Martins, Daniella Regina Arantes; Macedo, Gorete Ribeiro
Visceral leishmaniasis is an infecto-parasitarian disease caused by obligatory intracellular protozoa belonging to the genus Leishmania, and might be lethal since there is no precocious diagnosis and proper treatment. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Genetically modified micro-organisms are used industrially in general for production of hormone, antibiotics and proteins. In the production of heterologous molecules, both the expression and the production step are important [2]. Therefore, the study of cultivation conditions in bioreactor plays an important role for the process viability of batch and fed-batch [3]. Thus, the aim of this work was to study strategies for production of the 503 antigen of Leishmania infantum chagasi using cultivation in batch and fed-batch
In vitro anticoagulant and antioxidant activities of Jatropha gossypiifolia L. (Euphorbiaceae) leaves aiming therapeutical applications
(BMC, 2014-10-20) Félix-Silva, Juliana; Souza, Thiago; Câmara, Rafael Barros Gomes da; Cabral, Bárbara; Silva-Júnior, Arnóbio Antônio da; Rebecchi, Ivanise Marina Moretti; Zucolotto, Silvana Maria; Rocha, Hugo Alexandre de Oliveira; Fernandes-Pedrosa, Matheus de Freitas
Background: Jatropha gossypiifolia L. (Euphorbiaceae) is a medicinal plant largely used in folk medicine. Teas from the leaves are popularly used as an antithrombotic agent and the branches are frequently employed as a “thick blood” agent. Considering that the anticoagulant activity associated with antioxidant properties could be beneficial for various cardiovascular diseases, this study’s aim is the evaluation of anticoagulant and antioxidant activities of J. gossypiifolia leaves, seeking new therapeutic purposes for this plant. Methods: The aqueous leaf crude extract (CE) was prepared by decoction and was fractionated by liquid-liquid partition with solvents of increasing polarity. The phytochemical analysis was performed by thin layer chromatography (TLC) and by the spectrophotometric quantification of sugars, proteins and phenolic compounds. The anticoagulant activity was evaluated by prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests. The capacity to act in the fibrinolytic system (fibrinolytic and fibrinogenolytic activities) was also assessed. The antioxidant activity was evaluated by total antioxidant capacity, reducing power, copper chelating activity, iron chelating activity, hydroxyl radical scavenging activity and superoxide radical scavenging assays. The potential toxicity was evaluated using hemolytic assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay on HEK-293 cells. Results: CE showed significant anticoagulant activity in aPTT test, while no action was observed in PT test, suggesting a preferential action toward the intrinsic and/or common pathway of coagulation. No effect was observed in the fibrinolytic system. Using the aPTT test, it was observed that the residual aqueous (RA) fraction was the most active, being two times more active than CE. RA presented very significant antioxidant activity in all models tested comparable to or even higher than CE. Regarding the safety, CE and RA did not produce significant cytotoxicity in both tests employed. Phytochemical analysis revealed the presence of alkaloids, flavonoids, proteins, tannins, steroids and/or terpenoids and sugars. Conclusions: CE and RA possessed significant anticoagulant and antioxidant activity and absence of cytotoxic effect in vitro, thus showing the potential of the plant, especially RA fraction, as a new source of bioactive molecules for therapeutic purposes, with particular emphasis on the treatment of cardiovascular diseases
Optimization of culture medium for cell growth and expression of 648 antigen from Leishmania infantum chagasi in recombinant Escherichia coli M15
(BioMed Central Ltd, 2014-11-20) Santos, Everaldo Silvino dos; Vaz, Michelle Rossana Ferreira; Sousa Junior, Francisco Canindé de; Costa, Letícia Maia Resende; Martins, Daniella Regina Arantes; Macedo, Gorete Ribeiro de
Visceral leishmaniasis is a potentially fatal disease caused by Leishmania infantum chagasi in the New World. Anti-leishmanial treatment is problematic because antileishmanial drugs are toxic and costly and drug resistance is emerging. Moreover, there is no effective and safe vaccine approved for human use against any form of leishmaniasis. It seems likely that both a subunit vaccine and an optimized diagnostic test would benefit from the production of recombinant antigens. However, for recombinant protein production, the composition of the cell growth medium must be carefully formulated and monitored, because it may have significant metabolic effects on both the cells and protein expression. Thus, the aim of this study was to optimize the culture medium for cell growth and the expression of the 648 antigen from Leishmania i. chagasi in recombinant Escherichia coli M15. The induction allowed the expression of the 648 antigen; this was confirmed by electrophoresis, which showed a protein with a molecular mass of 24 kDa. The results showed that the higher cell concentration obtained in TB medium can be explained by the larger number of components such as amino acids and vitamin precursors contained in this complex medium, as well as glycerol as a carbon and energy source. Acetic acid excretion, detected at the end of fermentation, resulted in an inhibitory effect for both cell growth and protein production, because this was above the concentration limit of 0.9 g L−1
Armazenamento de Medicamentos em Unidades Básicas de Saúde de Macaíba, RN, Brasil
(Conselho Federal de Farmácia - CFF, 2016-03-31) Simão, Renato Wagner Tinôco de Sousa; Batista, Almária Mariz
Este estudo avaliou o armazenamento de medicamentos em 22 unidades básicas de saúde do município de Macaíba, Estado do Rio Grande do Norte, no período de janeiro a março de 2012. Para isto, foi realizada verificação in loco da infraestrutura e observação da rotina do serviço destas unidades, além de aplicação de Roteiro de Inspeção, com base na RDC nº 44/09, o que proporcionou subsídios para contextualização do papel do farmacêutico frente à assistência farmacêutica no local. Para identificação e análise dos medicamentos com prazo de validade expirado, foram utilizadas a Relação Nacional de Medicamentos Essenciais e a Relação Municipal de Medicamentos Essenciais do município. Nenhuma das farmácias destas unidades apresentava responsável técnico farmacêutico, manual sobre armazenamento de medicamentos, controle de umidade e temperatura. Em todas as farmácias havia medicamentos com prazo de validade expirado, abrangendo 1395 unidades mínimas não fracionadas, pertencendo a 31 classes farmacológicas diferentes e abrangendo 34 especialidades farmacêuticas. Os resultados revelam a precariedade em que se encontram estas unidades e, consequentemente, de suas respectivas farmácias, o que pressupõe a necessidade de atuação mais intensa por parte de gestores, vigilância sanitária e Conselhos Regional e Federal de Farmácia
Desenvolvimento e validação de indicadores de boas práticas de segurança do paciente: Projeto ISEP-Brasil
(Escola Nacional de Saúde Pública Sergio Arouca, Fundação Oswaldo Cruz, 2016-09-19) Batista, Almária Mariz; Gama, Zenewton André da Silva; Saturno-Hernández, Pedro Jesus; Freitas, Marise Reis de; Ribeiro, Denise Nieuwenhoff Cardoso; Medeiros, Paulo José de; Barreto, Analúcia Filgueira Gouveia; Lira, Benize Fernandes; Medeiros, Carlos Alexandre de Souza; Vasconcelos, Cilane Cristina Costa da Silva; Silva, Edna Marta Mendes da; Faria, Eduardo Dantas Baptista de; Dantas, Jane Francinete; Neto Júnior, José Gomes; Medeiros, Luana Cristina Lins de; Sicolo, Miguel Angel; Fonseca, Patrícia de Cássia Bezerra; Costa, Rosângela Maria Morais da; Monte, Francisca Sueli; Melo Neto, Veríssimo de
Um monitoramento eficaz da segurança do paciente precisa focar a implantação de práticas baseadas em evidências que evitem danos desnecessários ligados à assistência à saúde. O objetivo do Projeto ISEP-Brasil foi desenvolver e validar indicadores de boas práticas de segurança do paciente para o contexto brasileiro. Tomou por base a tradução e adaptação dos indicadores validados no Projeto ISEP-Espanha, além do documento Safe Practices for Better Healthcare do National Quality Forum dos Estados Unidos, que possui 34 recomendações de boas práticas. Realizou-se validação por um painel de 25 especialistas e análise da confiabilidade e viabilidade em um estudo-piloto realizado em três hospitais com diferentes tipos de gestão (estadual, federal e privada). Aprovaram-se 75 indicadores de boas práticas (39 de estrutura; 36 de processo) para 31 das 34 recomendações. Os indicadores foram considerados válidos, confiáveis e úteis para o monitoramento da segurança do paciente em hospitais brasileiros
Mathematical modeling of the whole expanded bed adsorption process to recover and purify chitosanases from the unclarified fermentation broth of paenibacillus ehimensis
(Elsevier, 2016-12) Souza, Domingos Fabiano de Santana; Padilha, Carlos Eduardo de Araújo; Dantas, Paulo Victor Fortunato; Sousa Junior, Francisco Canindé de; Oliveira, Jackson Araújo de; Macedo, Gorete Ribeiro de; Santos, Everaldo Silvino dos
In this study, a general rate model was applied to the entire process of expanded bed adsorption chromatography (EBAC) for the chitosanases purification protocol from unclarified fermentation broth produced by Paenibacillus ehimensis using the anionic adsorbent Streamline® DEAE. For the experiments performed using the expanded bed, a homemade column (2.6 cm × 30.0 cm) was specially designed. The proposed model predicted the entire EBA process adequately, giving R2 values higher than 0.85 and 2 as low as 0.351 for the elution step. Using the validated model, a 33 factorial design was used to investigate other non-tested conditions as input. It was observed that the superficial velocity during loading and washing steps, as well as the settled bed height, has a strong positive effect on the F objective function used to evaluate the production of the purified chitosanases
Recovery and concentration of ortho-phenylphenol from biodesulfurization of 4-methyl dibenzothiophene by aqueous two-phase flotation
(Elsevier, 2017-04) Souza, Domingos Fabiano de Santana; Padilha, Carlos Eduardo de Araújo; Dantas, Paulo Victor Fortunato; Sousa Júnior, Francisco Canindé; Oliveira Júnior, Sérgio Dantas; Nogueira, Cleitiane da Costa; Oliveira, Jackson Araújo de; Macedo, Gorete Ribeiro de; Santos, Everaldo Silvino dos
In this study we focus on recovery and concentration of ortho-phenylphenol (OPP) from fermentation broth by aqueous two-phase flotation (ATPF). The effects of pH, ammonium sulfate concentration, OPP concentration, air flow-rate, flotation time and initial volume of organic phase were evaluated on process performance using multivariate statistical methodology. Under optimal conditions, the separation efficiency (E%), the concentration coefficient (a) and the distribution coefficient (D) for ATPF reached 57.55%, 4.23 and 9.86, respectively. When compared to others conventional techniques also used in this work, such as aqueous two-phase extraction, solvent sublation and liquid-liquid extraction, the ATPF showed better performance in terms of E%, a and D. Additionally, experiments carried out with fermentation broth derived from the biodesulfurization of 4-MDBT have shown promise in the recovery and concentration of OPP. In summary, the results indicated that the ATPF is an effective separation technique in the pretreatment of the OPP from bona fide mixtures
Baccharis trimera (Less.) DC exhibits an anti-Adipogenic effect by inhibiting the expression of proteins involved in adipocyte differentiation
(MDPI, 2017-06-12) Nascimento, Daniele de Souza Marinho do; Oliveira, Ruth Medeiros; Câmara, Rafael Barros Gomes da; Gomes, Dayanne Lopes; Monte, Jessika Fernanda Santiago; Costa, Mariana Santana Santos Pereira; Fernandes, Júlia Moraes; Langassner, Silvana Maria Zucolotto; Rocha, Hugo Alexandre de Oliveira
Baccharis trimera (Less.) DC (gorse) is a plant popularly used for the treatment of obesity. In this study, we prepared three B. trimera extracts aqueous extract (AE), decoction (AE-D), and methanol extract (ME) and investigated their antioxidant effects in six different tests and their anti-adipogenic effect in 3T3-L1 cells. The extracts showed a dose-dependent antioxidant activity in all tests. AE was the most potent antioxidant in copper and ferric ion chelation assays, whereas AE-D was the most potent in superoxide and hydroxyl radical scavenging assays, reducing power assay, and total antioxidant capacity analysis. Only ME showed a cytotoxic effect against 3T3-L1 cells. Lipid accumulation decreased in 3T3-L1 adipocytes in the presence of AE and AE-D extracts (0.5 to 1.0 mg/mL). In addition, the extracts dramatically attenuated the levels of adipogenic transcriptional factors, including CCAAT enhancer-binding protein α (C/EBPα), CCAAT enhancer-binding protein β (C/EBPβ), and gamma receptors by peroxisome proliferators (PPARγ), during adipogenesis. AE-D (1.0 mg/mL) caused an approximately 90% reduction in the levels of these molecules. We propose that B. trimera has an anti-adipogenic effect and could be used in the development of functional foods
Enhancing enzymatic hydrolysis of coconut husk through Pseudomonas aeruginosa AP 029/GLVIIA rhamnolipid preparation
(Elsevier, 2017-08) Santos, Everaldo Silvino dos; Araújo, Cynthia Kérzia Costa de; Campos, Alan de Oliveira; Padilha, Carlos Eduardo de Araújo; Sousa Júnior, Francisco Canindé de; Nascimento, Ruthinéia Jéssica Alves do; Macedo, Gorete Ribeiro de
This work investigated the influence of chemical (Triton X-100) and biological surfactant preparation (rhamnolipids) in coconut husk hydrolysis that was subjected to pretreatment with acid-alkali or alkaline hydrogen peroxide. The natural and pretreated biomass was characterized using the National Renewable Energy Laboratory protocol analysis as well as X-ray diffraction and scanning electron microscopy. The results demonstrated that in terms of the total reducing sugars, there was no significant difference between the hydrolysis using Triton X-100 and rhamnolipids, regardless of the pretreatment. A cellulosic conversion value as high as 33.0% was obtained in experiments with rhamnolipids. The coconut husk was observed to be a potential biomass that could produce second generation ethanol, and the rhamnolipid preparation can be used to support for the enzymatic hydrolysis, enhancing the advantage of cellulose conversion into glucose over chemical surfactants because it is an environmentally friendly approach

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