Programa de Pós-Graduação em Ciências Biológicas
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Master Thesis Efeito da dipirona e do ácido acetilsalicílico sobre parâmetros espermáticos e na contração do ducto epidimário da região distal da cauda do epidídimo de rato(Universidade Federal do Rio Grande do Norte, 2021-08-06) Martins, Ana Beatriz Melo; Silva Júnior, Edilson Dantas da; https://orcid.org/0000-0001-5505-8056; http://lattes.cnpq.br/5459705151588106; http://lattes.cnpq.br/4250355474441363; Becari, Christiane; Rachetti, Vanessa de Paula SoaresDipyrone and acetylsalicylic acid (ASA) are cyclooxygenase inhibitors widely used worldwide for pain relief, fever and inflammation treatment (except dipyrone). However, the effects of dipyrone or AAS on male fertility are still not fully known, mainly considering the epididymis as a putative target for their anti-fertility effects. Therefore, the aim of this study was to evaluate the effects of dipyrone and ASA on sperm parameters, serum testosterone levels and epididymal duct contractions. We used epididymal ducts isolated from epididymis distal cauda from adult male Wistar rats for studying the in vitro effects of dipyrone and ASA (10, 100 and 1000 µM) on contractions induced by phenylephrine, carbacol or KCl. In addition, we treated adult male Wistar rats with free drug vehicle, dipyrone 100 mg/Kg or ASA 100 mg/Kg for 15 days in order to check the serum testosterone levels, sperm parameters (spermatid number, daily sperm production, sperm transit through epididymis and sperm concentration in epididymis) and epididymal duct contractions. We found that in vitro dipyrone 100 and 1000 µM decreased the maximum effect (Emax) for phenylephrine by about 20 and 40%, respectively. Pre-incubation with dipyrone 1000 µM was able to decrease the Emax for carbachol by 30% as well as reduce the potency for carbachol by 3-fold. Similarly, ASA 100 and 1000 µM decreased the Emax for phenylephrine by 20 and 30%, respectively. ASA 1000 µM also reduced the Emax and potency for carbachol by 20% and 2.5-fold, respectively. The in vivo treatment with dipyrone or AAS for 15 days were able to reduce the serum testosterone levels by about 60% for both drugs. We also found a significant reduction in the epididymal sperm number and daily sperm production. On the other hand, the sperm transit time through epididymis and epididymal duct contractions induced by phenylephrine or carbachol were unaltered. In conclusion, the in vivo treatment with dipyrone or ASA reduced the sperm count in epididymis and such effect could be related to a decreased daily sperm production induced by low testosterone levels. Furthermore, we were not able to found any between epididymal motor activity alterations and low epididymal sperm numbers in treated animals.Master Thesis Quimioterapia experimental antimalárica com extratos de pólen apícola de cocos nucifera desidratado(Universidade Federal do Rio Grande do Norte, 2022-01-31) Melo, Brena Karisa Campos de; Andrade Neto, Valter Ferreira de; http://lattes.cnpq.br/4863082845974813; http://lattes.cnpq.br/7226247859722492; Pontes, Daniel de Lima; https://orcid.org/0000-0001-7770-0492; http://lattes.cnpq.br/1903229358912987; Abramo, ClariceMalaria is an acute infectious tropical disease caused by Plasmodium parasites. There are five species of Plasmodium causing malaria in humans, P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi. In 2020, 241 million cases of malaria were registered, remaining as one of the main infectious diseases in the world. Most of the malaria research focus on the search for new antimalarial drugs, as there is ongoing recurrence of Plasmodium strains resistant to the current antimalarial treatment, including Artemisinin-based combination therapies (ACTs). The therapeutic use of medicinal plants has long contributed to the treatment of diseases. Regarding malaria treatment, many drugs such as quinine and artemisinin are derived from plants. Studies using bioactive compounds, extracted from plants, have shown biological activities such as anti-inflammatory, antimicrobial, antifungal, anticancer and insecticidal. In this context, the present work sought to investigate the antiplasmodial potential of an extract of bee pollen from Cocos nucifera. To analyze the suppression of parasitemia, female Swiss mice weighing 27 ± 2 g at 6 to 10 weeks of age were divided into groups of 5 animals each. Mice were infected with 1x106 of red blood cells parasitized by Plasmodium berghei ANKA, and treated orally, “per gavage” for 4 consecutive days. Five experimental groups were used: (I) negative control group treated with water; test groups with 250 mg/kg (II), 500 mg/kg (III) and 1000 mg/kg (IV) with bee pollen extract; and (V) positive control group with 25 mg/kg of chloroquine. Antimalarial activity was evaluated by the percentage of parasite growth inhibition in the test groups compared to the negative control group. Additionally, the in vivo assay had the cumulative mortality, acute toxicity of the extract and histopathological analysis performed; during the in vitro assay, the cytotoxic activity of the extract were evaluated in HepG2 cells, using six concentrations, from 0.01-1,000 μg/mL for 24 h. Analyzes of the extract chemical composition GC/MS and LC/MS were also performed. The analysis of the pollen extract chemical composition revealed the presence of fatty acids, coumarins, flavonoids and terpenes. In the in vivo acute toxicity test, no signs of toxicity and mortality were observed, as well as the histopathological analyzes showed no changes in relation to the control. In the in vitro cytotoxicity evaluation, it was possible to observe that the extract maintained 100% cell viability at all concentrations applied. The antiplasmodial assay demonstrated the suppression of parasitemia at concentrations of 500 mg/kg and 1000 m/kg, with 49% and 57% of suppression, respectively. In summary, it is possible to observe the promising results of bee pollen crude extract against Plasmodium infection. Treatment with the extract was able to significantly reduce parasitemia in mice, without inducing toxicity in vivo and in vitro. However, complementary studies are still necessary to isolate the extract bioactive components and evaluate its activity against Plasmodium strains.Master Thesis Avaliação da capacidade de extratos proteicos de Plasmodium falciparum em estimular a produção de anticorpos específicos e induzir hemólise de eritrócitos humanos não infectados na presença do sistema complemento(Universidade Federal do Rio Grande do Norte, 2021-01-22) Marcelino, Brenna Marceliane de Melo; Andrade Neto, Valter Ferreira de; http://lattes.cnpq.br/4863082845974813; Silva, Marcelo de Sousa da; https://orcid.org/0000-0002-1000-0149; http://lattes.cnpq.br/1295430560645312; Sales, Valéria Soraya de Farias; Moreno, Yorleydy RuizMalaria is a parasitic infectious disease, caused by protozoa of the genus Plasmodium and characterized by acute fever, chills, sweating and headache. six species cause human malaria in the world: P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi and P. simium. Most cases of severe human malaria are attributed to infections by Plasmodium falciparum, considered the most aggressive due to tissue hypoxia resulting from several factors, including severe anemia. According to the 2017 World Malaria Report, there were about 219 million cases of malaria and 435.000 deaths from the disease each year. As an intracellular parasite, Plasmodium depends on the host to survive and genetically determined factors can influence the individual's susceptibility to clinically developing malaria, especially those that result in hematological changes. Thus, the present study aims to evaluate the participation of the complement system in the genesis of the phenomenon of hemolysis induced by Plasmodium falciparum in in vitro models and, in the second moment, to develop and standardize in vitro methodologies for understanding the mechanism of hemolysis of treated human erythrocytes with total P. falciparum proteins mediated by the complement system. For this, the methodology includes the in vitro cultivation of the W2 strain of Plasmodium falciparum, in addition to animal experimentation using Swiss mice immunized with protein extract from the culture of P. falciparum for the production of polyclonal serum, in addition to autologous hemolytic assays and determination of fragments complement by flow cytometry. The protein profiles visualized through polyacrylamide gel electrophoresis (SDS-PAGE), allows the identification of soluble P. falciparum proteins. The immunization of animals with processed extracts of P. falciparum enabled the production of functional murine anti-P falciparum antibodies that can even be used as a polyclonal serum.Master Thesis O efeito de diferentes substratos na identificação de espécies de dípteros sarcosaprófagos em fragmentos de Mata Atlântica da cidade de Natal/RN(Universidade Federal do Rio Grande do Norte, 2020-09-10) Soares, Raimundo Nélio de Almeida; Corso, Gilberto; Barbosa, Taciano de Moura; http://lattes.cnpq.br/0377384091253781; https://orcid.org/0000-0003-1748-4040; http://lattes.cnpq.br/0274040885278760; http://lattes.cnpq.br/5052453294220193; Macedo Filho, Antonio de; Oliveira, Jonas Ivan NobreABSTRACT Insects perform many important functions in nature that help maintain balance in ecosystems. Muscomorpha diptera would be no different, they contribute directly to the ecosystem balance by accelerating the recycling of organic matter and allowing nutrients to be reused. This study aimed to evaluate the attractiveness of different substrates on the assembly of sarcosaprophagous dipterans in a preserved fragment of the Atlantic Forest. The collections were carried out in the State Park Dunas do Natal - RN and in a fragment of forest located inside the federal University of Rio Grande do Norte during the months of July and August 2019. For the collection of insects, suspended traps made from from a pet bottle, which were baited with five different types of substrates: fermented mango and cashew, decomposed chicken and fish liver, and fresh human feces. A total of 1,499 adults from eight families were collected. Calliphoridae was the most representative family with (32.49%) of the collected specimens, followed by Phoridae (16.54%), Fannidae (15.88%) and Sarcophagidae (13.88%). The other families: Drosophilidae, Muscidae, Micropezidae and Ulidiidae correspond to (21.21%) of the total of individuals. Among the substrates, chicken liver and human feces were the most attractive substrates represented (47.63%) and (23.08%) of adult flies, respectively. The substrates that proved to be less efficient in the locality were cashew (9.74%), fish (12.88%) and mango with (6.67%) of adults. The results also revealed that Lucilia eximia was the most abundant species in the study areas. The present work also contributed to the enrichment of the list of Diperas species known in the state of RN. If new records were found for the Muscidae families: Cariocamia maculatus (Snyder, 1951) and Helina sp; Sarcophagidae: Oxysarcodexia diana (Lopes, 1933), O. bakeri (Aldrich, 1916), Sarcofahrtiopsis cuneata (Townsend, 1935), Sarcophaga (Liopygia) crispola () and Tricharaea (Sarcophagula) canuta (Wulp, 1896) and also the record of 1896) family Micropezidae.Master Thesis Avaliação da atividade antibacteriana e antibiofilme in vitro de óleos essenciais de plantas condimentares sobre bactérias potencialmente patogênicas(Universidade Federal do Rio Grande do Norte, 2021-09-21) Barros, Amanda Vieira de; Motta Neto, Renato; http://lattes.cnpq.br/6909091962347443; http://lattes.cnpq.br/4893689651502868; Rêgo, Manoela Torres do; http://lattes.cnpq.br/0735782214033188; Bachur, Tatiana Paschoalette RodriguesIn the search for new alternatives for bacterial control, research using spice plants is gaining more and more prominence. Given their nutritional value and antibacterial potential, spice plants are being used in alternative methodologies. Thus, antimicrobials of natural origin could be an effective and economically viable alternative for food preservation, replacing the use of synthetic substances. Based on these researches, the main objective of the study was to evaluate the antibacterial and antibiofilm potential of essential oils from spice plants against strains of food interest Escherichia coli (ATCC 25922) Staphylococcus aureus (ATCC 25923), Klebsiella pneumoniae (ATCC 10031), Pseudomonas aeruginosa (CCBH 5698) and Salmonella Typhimurium. The plants Rosmarinus officinalis (Rosecrim), Zingiber officinale (Ginger), and Allium sativum (Garlic) were commercially obtained, and their oils were extracted by hydrodistillation coupled with Clevenger. Their chemical profiles were evaluated by the Gas Chromatography technique coupled with Mass Spectrometry. To evaluate the antimicrobial activity, the Minimum Inhibitory Concentration and, subsequently, the Minimum Bactericidal Concentration of essential oils were determined. The subinhibitory concentrations of MIC, 2xMIC, and 4xMIC were used to analyze the effect of oils in inhibiting biofilms, being evaluated by crystal violet uptake tests and optical density reading at 595 nm, and the results compared with the effect of the antibiotic Amoxicillin at the same concentrations. In evaluating the chemical profile of essential oils, a higher concentration of monoterpenes and sesquiterpenes was observed in R. officinalis and Z. officinale, while the oil of A. sativum was mainly composed of organosulfur substances. The essential oils showed activity against the strains tested, especially against P. aeruginosa (MIC 1.33-5.33 µg/mL and CBM 0.83-1 µg/mL). The best result of the oil of R. officinalis was observed against the S. aureus strain (MIC 8 µg/mL and MBC 3.33 µg/mL), whereas Z. officinale achieved a better effect against E. coli (MIC 4 µg/mL and MBC 5.33 µg/mL), while A. sativum oil had the best performance against K. pneumoniae (MIC 4 µg/mL and MBC 5.33 µg/mL). Assessing the effect of oils on biofilm inhibition, R. officinalis was able to inhibit only the S. aureus formation (≥ 57.33%) at the concentrations of 2xMIC and 4xMIC, with no effect on other biofilms. The best antibiofilm effects were observed for Z. officinale and A. sativum, in which they managed to inhibit the formation of almost all tested biofilms with an inhibition rate greater than 70%, achieving better performance than the antibiotic Amoxicillin at the same concentrations. It is concluded that, due to the concentration of terpenes and organosulfur, the essential oils of Z. officinale and A. sativum, respectively, showed better antibacterial activity, inhibiting the formation of biofilms and becoming promising alternatives for the preservation of food and combating pathogenic microorganisms.Master Thesis Avaliação por bioquímica quântica de interações entre o receptor beta secretase 1 (BACE1) e Ligantes(Universidade Federal do Rio Grande do Norte, 2021-12-02) Clemente Júnior, Washington Sales; Fulco, Umberto Laino; http://lattes.cnpq.br/9579151361576173; http://lattes.cnpq.br/6613096511935122; Macedo Filho, Antônio de; http://lattes.cnpq.br/5432651695056904; Lima Neto, José Xavier de; http://lattes.cnpq.br/4087097955623085Alzheimer's disease (AD) is a progressive multifactorial neurodegenerative disorder and the main cause of dementia in late adulthood. The amyloid cascade hypothesis suggests a deposition of beta amyloid (Aβ) in the brain, as a result, there is an imbalance between the production and clearance of the Aβ peptide deposited in diffuse plaques. This is the cause of damage in AD. In recent years, several drugs have been developed to reduce the concentrations of Aβ40 and Aβ42, including 4-Butoxy-3-chlorobenzyl imidothiocarbamate and Verubecestat, which have a great potential to minimize the production of the peptide. This work analyzes the main interactions that occur between a Beta secretase 1 (BACE1) and its ligands using the Molecular Fragmentation Method with Conjugated Caps (MFCC), using the DFT (Density Functional Theory) approach for identification of which amino acid residues contribute to the binding between the drugs, mentioned before, and receptor. The results showed the energetic values of each amino acid residue constituting the site of interaction with the ligands under analysis. This assessment took place within a radius of up to 10.0 Å away from the drugs. The results show that 4- Butoxy-3-chlorobenzyl imidothiocarbamate has greater affinity to the molecular target than Verubecestat. Residues ASP93, ASP289, TYR132, SER96, THR293, GLY291, LEU91, ILE179, THR292, TRP176, GLY95, PHE169, GLN134 are important for system formation with the two drugs. Of these, the ASP93 and ASP289 are key pieces for stabilizing the formation of complexes. The data found are essential for creating new strategies in the development of drugs that can contribute to improving the quality of life of patients with AD.Master Thesis Análise in silico da interação entre a bomba de efluxo ACRABTOLC e inibidores(Universidade Federal do Rio Grande do Norte, 2021-12-03) Batista, Sabrynna de Oliveira; Fulco, Umberto Laino; Melo, Maria Celeste Nunes de; 34195602300; http://lattes.cnpq.br/0580551464788795; http://lattes.cnpq.br/9579151361576173; http://lattes.cnpq.br/2697949543491903; Barbosa Filho, Francisco Ferreira; http://lattes.cnpq.br/2664045440231962; Bezerra, Katyanna Sales; http://lattes.cnpq.br/5246433025467179Efflux pumps are an important defense mechanism that bacteria use to evade antibiotic treatment methods used in medicine to treat infectious diseases. For many years, research groups have been developing new drugs in order to change the current scenario of multiresistance that these microorganisms present, making it difficult for patients to improve. AcrAB-TolC is a protein complex belonging to the RND family, with important distribution in bacteria classified as priority agents for the development of combat methods. Studies using efflux pump inhibitor molecules have been developed as an alternative method to combat multiresistant bacteria. The basis of these inhibitors is the enhancement of existing antibiotic methods. Thus, this study aims to perform ab initio analysis of the interaction of MBX2319 inhibitors and their derivatives with the AcrAB-TolC complex, through quantum mechanics calculations, using the Density Functional Theory (DFT). The calculations of the interaction energies of the involved residues were made using the Molecular Fragmentation with Conjugate Caps (MFCC). The results presented demonstrate the presence of hydrogen bonding that enhances the strength of interaction between ligands analyzed in the AcrAB-TolC complex. Furthermore, the Phe178 residue presented significant results in the 3 analyzed ligands, highlighting its importance for the total energy of interaction. As the main residues involved in the binding of AcrAB-TolC with the antibiotic minocycline, used for comparative analysis, we have ILe277, Phe178, Gly179, Phe615, Arg620, Glu273, Asn274, Leu177 and Ser48. As for the MBX2319 linker, the main residues were Phe178, Phe628, Val139, Phe610, Phe136, Phe615, Tyr327, Val612 and Phe617. Finally, the MBX3132 linker, derived from MBX2319, had as main residues Phe178, Phe628, Phe615, Phe136, Phe610, Val612, Tyr327, ALa286 and ILe277. With these results, we can understand how to improve the treatment methods used to help combat the action of this bacterial resistance mechanism, and thus give light to future research.Master Thesis Situação da Lontra longicaudis no Rio Grande do Norte: habitat e estratégias para conservação na Mata Atlântica nordestina do Brasil(Universidade Federal do Rio Grande do Norte, 2021-09-23) Sousa, Rafael Turíbio Moraes de; Corso, Gilberto; http://lattes.cnpq.br/0274040885278760; http://lattes.cnpq.br/3893867147648686; Venticinque, Eduardo Martins; http://lattes.cnpq.br/3582966116563351; Silva, Flávio José de Lima; http://lattes.cnpq.br/1421802360229451; Gavilan, Simone Almeida; http://lattes.cnpq.br/6556168670883302The Otter longicaudis (Olfers, 1818) is a carnivorous mammal representing the Mustelidae family and the Lutrinae subfamily with interdigital membranes on the toes that allow an excellent adaptation to the semiaquatic lifestyle. It is a skittish animal and difficult to see, due to its nocturnal and twilight habits, mainly in the Northeast of Brazil. One of the ways to study the species' habitat is through indirect observation, along with traces such as carcasses, tracks, latrines, anal mucus and feces. There are several species of mustelids that interact with fishing populations throughout the world, causing conflicts between humans and animals, however there are few studies that address this issue in Brazil. Although the distribution of the species is considered by the IUCN (International Union for Conservation of Nature) as "Insufficient Data", recent studies describe that the species is classified as "Near Threatened" in Northeastern Brazil and "Vulnerable" in the Atlantic Forest domain due to the high level of habitat degradation, specifically the deforestation of river banks, by overfishing, fish farming and shrimp farming. Aiming at the description of the habitat of the Lontra longicaudis in Rio Grande do Norte, a broad photographic record of the habitat of L. longicaudis, with emphasis on the latrines, burrows, places of passage of individuals and typical feeding places. Later, aiming to minimize the conflict between otters and fishermen in the riverside region of the State, we proposed a solution to improve the fishing gear (pits) of fishermen destroyed by otters. For this purpose, cycles of non-systematic interviews (ICMBio authorization nº 32910) were carried out in five fishing communities in the Bonfim-Guaraíras Environmental Protection Area, between the years 2018 and 2019, which still fish for shrimp from the Macrobrachium sp. (Order: Decapoda; Family: Palaemonidae) using artisanal pots made with material from local vegetation. The main concern pointed out was the interest of L. longicaudis in the holes used for fishing, which are periodically broken by the otter. So, a technique that uses PVC pipes to make the pots was presented and readily accepted by the riverside population of APA BonfimGuarairas/RN, which promptly achieved success for artisanal fishing and generated subsidies for the conservation of L. longicaudis. Among the ideas developed and discussed with the riverside population, a series of public measures is needed, in particular: improving the current legislation; greater investment in education and environmental protection program; improved control of pollution and deforestation. Finally, an Otter Strategies and Actions for the state of Rio Grande do Norte was designed to be an essential tool for guiding research, fomenting ecological data and efficient and balanced management of areas inhabited by the neotropical otter.Master Thesis Avaliação dos efeitos comportamentais da retirada da sacarose em curto e longo prazo em ratas adolescentes(Universidade Federal do Rio Grande do Norte, 2021-09-20) Moura, Kariely Gonçalves de; Rachetti, Vanessa de Paula Soares; http://lattes.cnpq.br/1524215726056886; http://lattes.cnpq.br/5225490144754617; Bortoli, Valquíria Camin de; http://lattes.cnpq.br/3992098322056730; Silva Júnior, Edilson Dantas da; http://lattes.cnpq.br/5459705151588106Sugar (sucrose) is a highly flavorful and rewarding substance. Its consumption usually causes sensations, such as an improvement in mood and a decrease in agitation. Physiologically, the ingestion of highly palatable foods increases dopamine in the nucleus accumbens and the decrease in dopamine signaling leads to reduced activity in this region, which can happen in anxiety-related behaviors. The present study tested the hypothesis that sucrose withdrawal after prolonged exposure would favor anxietyrelated behaviors in adolescent females. Adolescent rats (30 days) were treated with sucrose at a concentration of 5% free choice for 16 days, with the exception of the control group, which was exposed only to water. After 16 days, sucrose was replaced by water according to two protocols. For the Short-term Withdrawal group, sucrose was replaced by water for 48h-72h prior to testing. For the Long-term Withdrawal group, sucrose was replaced by water for 21-22 days. After the withdrawal period, the animals were taken to the elevated plus maze and open field behavioral tests. In the elevated plus-maze, the long-term withdrawal group had its exploration time reduced compared to the control group (treated with water only) and continuous sucrose. The long-term withdrawal group entered the closed arms more often compared to the control and short-term withdrawal groups. In the open field, there was no treatment effect on the three parameters analyzed during the first five minutes of the test: number of entries into the center, time spent in the center and distance covered in the center. In conclusion, long-term sucrose withdrawal produces anxious-like behaviors and increases locomotion in the elevated plus-maze. More studies should be carried out to understand the neural bases involved with anxiety in sucrose withdrawal.Master Thesis Avaliação da atividade anticoagulante e bloqueadora de elastase de um inibidor de tripsina isolado de Caesalpinea pyramidalis Tul.(Universidade Federal do Rio Grande do Norte, 2017-09-28) Cruz, Joelton Igor Oliveira da; Uchoa, Adriana Ferreira; Santos, Elizeu Antunes dos; 41305655400; http://lattes.cnpq.br/6762251930590306; http://lattes.cnpq.br/6644671747055211; http://lattes.cnpq.br/2448189217304566; Medeiros, Caroline Addison Carvalho Xavier de; http://lattes.cnpq.br/2982271986555450; Rabêlo, Luciana Maria Araújo; http://lattes.cnpq.br/2165223941043909Plants are subject to a diversity of unfavorable biotic and abiotic conditions, undergoing constantly some evolutionary adaptation, that can contribute to its reproduction and survival. The development of sophisticated defense strategies against pests and pathogens such as the synthesis of several natural bioactive compounds stands out as part of this adaptive biological arsenal. A key group of bioactive molecules in this context are the protease inhibitors, which compromise the activity of digestive enzymes of herbivores, as well as an action of pathogens, contributing to the balance of biological interactions. Protease inhibitors, are important molecules because of their heterologous potential in other biological systems, and may have in vitro and in vivo effects on several biological models, as well as therapeutic properties in a range of disorders that compromise human health. In this work an inhibitor of serinoprotease (CpTI), was isolated from the Caesalpinia pyramidalis Tul. seeds by fractionation of the crude extract with 30- 60% ammonium sulfate, followed by separation in affinity chromatography on TrypsinSepharose 4B-CNBr-actived column, the retained peak was enriched in inhibitory activity for trypsin, chymotrypsin and elastase serine protease type. The thermostability test showed that CpTI was able to maintain its functionality up to 80 °C and the concentrations required to inhibit 50% of the activity of the trypsin, human neutrophil elastase and chymotrypsin enzymes were 17, 21 and 156 μg/mL, respectively. CpTI also prolonged the coagulation time by the intrinsic pathway (TPPa) in more than 80 seconds at a concentration of 36.2 μg / mL. It was also observed that CpTI has no cytotoxic activity to red blood cells at any of the concentrations tested (0,03 , 0,125 e 0,5 mg/mL). These results demonstrate the potential therapeutic use of CpTI in processes involving inflammation and coagulation.