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Title: Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defect increased FGF-2, osteocalcin and OPG immunostaining and IL-10 levels
Authors: Vasconcelos, Roseane Carvalho
Ferreira, Camyla
Araújo, Eduarda Medeiros de
Motta, Fabiana Villela da
Delmonte, Mauricio Roberto Bomio
Araújo Júnior, Raimundo Fernandes de
Paiva, Daniel Felipe Fernandes
Pirih, Flavia Q.
Silva, José Sandro Pereira da
Chan, Alan B.
Cruz, Luis J.
Ishii, Makiko
Medeiros, Caroline Addison Carvalho Xavier de
Guerra, Gerlane Coelho Bernardo
Araújo, Aurigena Antunes de
Keywords: Zirconia;Hydroxyapatite;Scaffold;Calvaria defect
Issue Date: 15-Jan-2020
Publisher: American Journal of Translational Research
Citation: VASCONCELOS, Roseane Carvalho.; FERREIRA, Camyla.; ARAUJO, Eduarda Medeiros de.; MOTTA, Fabiana Villela da; BOMIO, Maurício Roberto Delmonte; ARAUJO JUNIOR, Raimundo Fernandes de; PAIVA, Daniel Felipe Fernandes; PIRIH, Flávia Q.; SILVA, José Sandro Pereira da; CHAN, Alan B.; CRUZ, Luís J.; ISHII, Makiko; MEDEIROS, Caroline Addison Carvalho Xavier de; GUERRA, Gerlane Coelho Bernardo; ARAUJO, Aurigena Antunes de. Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defect increased FGF-2, osteocalcin and OPG immunostaining and IL-10 levels. American Journal of Translational Research, Wisconsin, v. 12, p. 2439-2450, 2020. Disponível em: Acesso em: 14 mai. 2021.
Portuguese Abstract: The aim of this study was to characterize and evaluate zirconia/hydroxyapatite in a critical size calvarial defect model in rats. Zirconia/hydroxyapatite (80/20) scaffold was characterized by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Critical size (8 mm) calvarial defects were created in wistar rats (n=48) and divided into four groups (90 days): G0 Group: positive control; G1 Group: hydroxyapatite; G2 Group: Zirconia; G3 Group: Zirconia/hydroxyapatite (80/20). Calvaria were subjected to Micro CT, histological and immunohistochemical analyses (RANK, RANKL, OPG, osteocalcin and FGF-2). IL-1 beta, IL-10 and TNF-alpha levels were analyzed by Elisa Immunoassay. The XRD analysis confirmed the formation of a crystalline structure and SEM showed the presence of regions corresponding to Zirconia and Hydroxyapatite. The Micro CT showed increased bone volume (BV/TV) and bone mineral density (BMD) in the G3 group (P<0.05). In addition, discrete periosteal bone formation was found at the interface of the defect edge and the external surface of the scaffold in the G3 group, showing osteocytes inside and osteoblasts (P<0.05) with scarce mononuclear inflammatory cells (P<0.01) in the central region of the defect. The immunostaining was moderate for RANKL, Osteocalcin and FGF-2 in the G3 group (P<0.5), while it was intense for OPG (P<0.001). IL-1 beta levels were decreased and IL-10 levels increased (P<0.05). Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defects increased bone density, osteoblast and osteoclast cell numbers, FGF-2, osteocalcin and OPG immunostaining and IL-10 levels
ISSN: 1943-8141
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