1. Universidade Federal do Rio Grande do Norte
2. Centro de Tecnologia
3. DEMAT - Departamento de Engenharia de Materiais
4. CT - DEMAT - Artigos publicados em periódicos

Título:	Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defect increased FGF-2, osteocalcin and OPG immunostaining and IL-10 levels
Autor(es):	Vasconcelos, Roseane Carvalho Ferreira, Camyla Araújo, Eduarda Medeiros de Motta, Fabiana Villela da Delmonte, Mauricio Roberto Bomio Araújo Júnior, Raimundo Fernandes de Paiva, Daniel Felipe Fernandes Pirih, Flavia Q. Silva, José Sandro Pereira da Chan, Alan B. Cruz, Luis J. Ishii, Makiko Medeiros, Caroline Addison Carvalho Xavier de Guerra, Gerlane Coelho Bernardo Araújo, Aurigena Antunes de
Palavras-chave:	Zirconia;Hydroxyapatite;Scaffold;Calvaria defect
Data do documento:	15-Jan-2020
Editor:	American Journal of Translational Research
Referência:	VASCONCELOS, Roseane Carvalho.; FERREIRA, Camyla.; ARAUJO, Eduarda Medeiros de.; MOTTA, Fabiana Villela da; BOMIO, Maurício Roberto Delmonte; ARAUJO JUNIOR, Raimundo Fernandes de; PAIVA, Daniel Felipe Fernandes; PIRIH, Flávia Q.; SILVA, José Sandro Pereira da; CHAN, Alan B.; CRUZ, Luís J.; ISHII, Makiko; MEDEIROS, Caroline Addison Carvalho Xavier de; GUERRA, Gerlane Coelho Bernardo; ARAUJO, Aurigena Antunes de. Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defect increased FGF-2, osteocalcin and OPG immunostaining and IL-10 levels. American Journal of Translational Research, Wisconsin, v. 12, p. 2439-2450, 2020. Disponível em: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7344105/. Acesso em: 14 mai. 2021.
Resumo:	The aim of this study was to characterize and evaluate zirconia/hydroxyapatite in a critical size calvarial defect model in rats. Zirconia/hydroxyapatite (80/20) scaffold was characterized by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Critical size (8 mm) calvarial defects were created in wistar rats (n=48) and divided into four groups (90 days): G0 Group: positive control; G1 Group: hydroxyapatite; G2 Group: Zirconia; G3 Group: Zirconia/hydroxyapatite (80/20). Calvaria were subjected to Micro CT, histological and immunohistochemical analyses (RANK, RANKL, OPG, osteocalcin and FGF-2). IL-1 beta, IL-10 and TNF-alpha levels were analyzed by Elisa Immunoassay. The XRD analysis confirmed the formation of a crystalline structure and SEM showed the presence of regions corresponding to Zirconia and Hydroxyapatite. The Micro CT showed increased bone volume (BV/TV) and bone mineral density (BMD) in the G3 group (P<0.05). In addition, discrete periosteal bone formation was found at the interface of the defect edge and the external surface of the scaffold in the G3 group, showing osteocytes inside and osteoblasts (P<0.05) with scarce mononuclear inflammatory cells (P<0.01) in the central region of the defect. The immunostaining was moderate for RANKL, Osteocalcin and FGF-2 in the G3 group (P<0.5), while it was intense for OPG (P<0.001). IL-1 beta levels were decreased and IL-10 levels increased (P<0.05). Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defects increased bone density, osteoblast and osteoclast cell numbers, FGF-2, osteocalcin and OPG immunostaining and IL-10 levels
URI:	https://repositorio.ufrn.br/handle/123456789/33246
ISSN:	1943-8141
Aparece nas coleções:	CB - DBF - Artigos publicados em periódicos CB - DMOR - Artigos publicados em periódicos CT - DEMAT - Artigos publicados em periódicos

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