Prospecção do potencial bioativo das sementes de Amburana cearensis em ensaios ovicida, antiviral e fungistático

Abstract

Amburana cearensis, usually known as Cumaru, this is a legume of the Caatinga biome, commonly used in traditional medicine in the treatment of symptomatology of various diseases,generally stem bark, leaves and seeds are the most used parts. The seeds have an arsenal of natural compounds with bioactive properties, and among these molecules, proteins emerge because they have so much importance in the defense of the embryo and as a source of nutrients, being targets of pharmacology studies. Lectins, vicilin, protease inhibitors and peptides are examples of defense proteins present in seeds, investigated with regard to potential microbial, viral replication inhibitors and entomotoxic. The objective of this work was the bioprospecting of biological activities of crude extract and protein fractions obtained from plant seeds A. cearensis.The concentrations of the samples used in hatching tests of larvae of Aedes aegypti, in determining the inhibition of viral replication DENV2 infecting VERO cells and the growth of fungi of the genus Candida and Trichosporon were previously defined by the MTT, in which we noted that 3,12 mg/ ml of the crude extract interferes less the viability of Vero cells and shows haemolytic activity. We verified then that the water phase soluble fraction F3 inhibited in 100% hatching of larvaeA. aegypti. The crude extract was effective in reducing the replication of DEN2 when dealing VERO cells after viral adsorption time, characterized antiviral effect. The F1 fraction had fungioestático effect inhibiting filamentation in Candida albicans, C. glabrata and Trichosporon asahii. The results reinforce the potential of bioactive A. cearensis as a source of molecules with bioactive properties against pathogens and vectors. However further studies should be conducted to identify the molecules present in the extracts and their mechanisms of action against fungi, viruses, and on the mosquito A. aegypti.