Avaliação do efeito do uso de creatina na movimentação ortodôntica em ratos

Abstract

Introduction:Considering that creatine (Cr) can interfere in several aspects of bone metabolism, it has been questioned whether this substance could also influence orthodontic movement, since this process depends on the shiftseebone, with bone apposition in the alveolar region and bone resorption on the opposite side. Objective: To evaluate the effect of creatine on orthodontic tooth movement in rats, considering the amount of tooth movement, alveolar bone volume and bone remodeling. Methodology: This is an experimental, in vivo, controlled study, with a descriptive and inferential basis. The sample consisted of 30 healthy male Wistar rats (Ratus norvegicus albinus), aged between 7 and 12 weeks, which were subjected to continuous orthodontic tooth movement of the upper first permanent molars, on the left side, for a period of 21 days. The rats were randomly divided into 3 groups: Control group (CG) - rats subjected only to orthodontic movement and daily water intake (n=8); Experimental group creatine 1 (GEC1) - rats subjected to orthodontic tooth movement and a daily dose of 21 mg/kg of creatine (n=10); and Experimental group creatine 2 (GEC2) - rats subjected to orthodontic tooth movement and a daily dose of 51 mg/kg of creatine (n=12). The analysis of the amount of tooth movement and alveolar bone volume was performed using computed microtomography. To investigate bone remodeling, a morphological study was performed using the hematoxylin-eosin (HE) method. The one-way ANOVA test was used for statistical analysis, followed by the Tukey test with a 95% confidence interval. Results: There were no statistically significant differences (p>0.05) between the control group and the experimental groups regarding the amount of tooth movement, alveolar bone volume, and bone remodeling of the rats subjected to orthodontic tooth movement. Conclusion: The findings of this study suggest that creatine does not affect orthodontic movement at the doses tested.