Incorporação do extrato de Ipomoea pes-caprae em sistema de nanoemulsão de óleo de soja e avaliação de citotoxicidade e atividade antioxidante

Abstract

Ipomoea pes-caprae is a plant species recognized in folk medicine for its various therapeutic activities. Recent studies have affected its topical efficacy, especially in relation to its anti-inflammatory, healing and anti-venom properties. Faced with these activities, the question arises about the behavior of a nanoemulsion formulation (which would enable greater penetrability, organoleptic improvement and bioavailability) containing the extract of this plant. The present study aims to use a phase inversion nanoemulsion containing the plant extract and analyze its physicochemical and biological characteristics, including, by DLS, droplet size (145nm), polydispersion index (0.122), cytotoxicity assays by hemolytic assay, crystal violet, which did not show significant toxicity, antioxidant activity by DPPH and ABTS, demonstrating the antioxidant activity, and the stability of the formulation, considering the pH, phenolic content, droplet size and polydispersion index during the period of 4 weeks, showing no significant variation, suggesting the stability of the formulation. The results indicate that the formulation is stable, presents low toxicity and preserves the properties of the extract, presenting more pleasant organoleptic characteristics, milder odor, lighter color, and texture similar to a lotion. However, more detailed studies are needed to ensure safety and accelerated and prolonged stability studies.