Nanopartículas híbridas lipídico-poliméricas contendo coenzima-Q10 para potencial atividade anticâncer

Abstract

Cancer is a disease of high incidence and prevalence all over the planet and its treatment is quite problematic due to the high toxicity degree of the drugs used, difficulty in targeting and limitations related to evasive mechanisms of tumors. Coenzyme Q-10 is an endogenous antioxidant molecule, highly lipophilic and widely studied for several applications, including its potential anticancer effect. Nanotechnology is used as a device to minimize these problems and be able to promote safer, more effective and humanized treatment for patients. Within nanotechnology, hybrid lipid-polymeric nanoparticles are new systems that combine the versatility of polymers with the biocompatibility of lipids, producing systems that bring out the best of both structuring agents. It was aimed to obtain and characterize NPH of PLGA and cholesterol loaded with CoQ10. In this work, was caried out the incorporation of Coenzyme Q-10 into lipid-polymeric cholesterol/PLGA hybrid nanoparticles, stabilized with Span 80® and Tween 80®, by the nanoprecipitation method. The characterization of these nanoparticles was evaluated in terms of diameter, polydispersity index (PdI) zeta potential (PZ) and pH, and the same parameters were used to measure the system stability during 45 days. The of Coenzyme Q-10 encapsulation efficiency was measured by an indirect method, by VIVASPIN® filters with 10 KDa cut-off, and a hemolysis test was performed to assess the system's compatibility with erythrocytes. Nanoparticles were obtained with a size between 100-200 nm, with low PdI (around 0.1), the PZ showed that the nanoparticles produced are anionic with a surface charge around 18 mV, with encapsulation efficiency above 90% and a high degree of hemolysis was observed as a preliminary result. The systems were successfully obtained and characterized, allowing progress in the study, seeking improvements and new tests.