Efeitos da água de coco hipertônica e sinvastatina no tratamento da sepse e choque hipovolêmico em ratos

Abstract

Resuscitation and treatment of shock and sepsis are controversial and challenging. The aim of this study was to examine the effects of coconut water + Nacl 3%+ simvastatin in resuscitation in a murine model of sepsis and hemorrhagic shock. In methods, adult male Wistar rats were subjected to sepsis by cecal ligation and puncture and hemorrhagic shock assigned to four fluid replenishment groups. Animals received 1. saline 0,9%; 2. coconut water (CW) + NaCl 3%; 3. coconut water + NaCl 3% + simvastatin (microemulsion)10 mg/Kg i.v; 4. Coconut water. Clinical score was determined and lung tissue was assessed for inflammation. Serum cytokines TNF-α, IL-1 β and leucocytes were measured. The results of this study indicated that coconut water+NaCl 3%+ simvastatin decreased significantly serum cytokines, survival, biochemical analysis and lung histopathological testing when compared to saline, water+NaCl 3% and coconut water. In conclusion, resuscitation with coconut water with Nacl 3%+simvastatin had a significant beneficial effect on downregulating cytokines and decreasing lung injury in a rat model of abdominal sepsis and hemorrhagic shock. We also demonstrated that coconut water with Nacl 3%+simvastatin administration clearly made liver and kidney function better and improved clinical score.