Potencial anti-inflamatório e antioxidante do Heparinoide isolado do camarão Litopenaeus vannamei

Abstract

The inflammatory response is a process of defense of the organism, however its exacerbated action generates an imbalance, making necessary the use of antiinflammatory compounds. The search for new molecules that act on the inflammatory and antioxidant response has intensified. In this scenario, HL-GAG, purified from shrimp has demonstrated ability to modulate various processes of the inflammatory response, an interest in evaluating the production of cytokines and the reactive metabolites has arise, since these molecules have their production increased during the process inflammatory. For this, we evaluated the leukocyte migration and the levels of cytokines in a model of peritonitis induced by LPS. Then, we investigated a compound cytotoxicity, nitric oxide production and intracellular reactive species in LPS-induced macrophages. Lastely, by performing the antioxidants of the testes, determining a chelation of ferrous ions, as well as an inhibition of reactive species, superoxide anion and hydroxyl radical. In addition, we evaluated lipid peroxidation and radical DPPH. The purification results related to HL-GAG were extracted efficiently. The compound also reduced the leukocyte rate by 50%, to the injury site. In addition to reducing the levels of all cytokines tested, with emphasis on TNF-α, which decreased by 57%. HL-GAG maintained 90% cell viability, even at the highest concentration (100 μg/mL), reduced the production of nitric oxide, and intracellular reactive species, suggesting an antioxidant action. In the ferrous ion chelation test, HL-GAG reduced the levels of this ion by 48% at 100 μg/mL. The compound also decreased the production of superoxide anion and hydroxyl radical. In lipid peroxidation, HL-GAG inhibited about 58% of lipid oxidation at the lowest concentration tested (25 μg/mL). With respect to DPPH, HL-GAG showed a reduction of approximately 70% in the lowest concentration. Therefore, we may suggest that HL-GAG would be modulating the inflammatory response via cytokines and antioxidant mechanisms.