Natural latex serum: characterization and biocompatibility assessment using galleria mellonella as an alternative in vivo model

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dc.contributor.authorBrasil, Giovana Sant’Ana Pegorin
dc.contributor.authorBarros, Patrícia Pimentel de
dc.contributor.authorMiranda, Matheus Carlos Romeiro
dc.contributor.authorBarros, Natan Roberto de
dc.contributor.authorJunqueira, Juliana Campos
dc.contributor.authorGomez, Alejandro
dc.contributor.authorHerculano, Rondinelli Donizetti
dc.contributor.authorMendonça, Ricardo José de
dc.contributor.authorIDhttps://orcid.org/0000-0003-4885-2811pt_BR
dc.date.accessioned2022-04-01T13:44:04Z
dc.date.available2022-04-01T13:44:04Z
dc.date.issued2021-12-30
dc.description.resumoNatural latex serum (NLS) is one of the natural rubber latex fractions from Hevea brasiliensis tree, which is formed by centrifuged serum and is composed of proteins, acids, nucleotides, salts and carbohydrates. The proteins present in NLS have demonstrated several interesting biological properties, including angiogenic, healing, osteogenic, anti-inflammatory, antimicrobial, in addition to inducing neovascularization, bone formation and osseointegra tion. Thus, we proposed to characterize NLS by physicochemical techniques and to investigate the biocompatibility by toxicological assays and safety test in Galleria mellonella. Infrared spectrum showed vibrational bands characteristic of amide I, II and III that are linked to the protein content, which was confirmed by the High Performance Liquid Chromatography profile and by the Electrophoresis analysis. This material did not exhibit hemolytic (rate <0.5%) and cytotoxic effects (viability >70%) and was able to enhance the proliferation of fibroblasts (>600%) after 3 days. The pronounced proliferative effect observed in fibroblast cells can be explained by the presence of the fibroblast growth factor (FGF) like protein revealed by the Western blot test. Moreover, NLS did not provoke toxic effects (survival ~ 80%) on the G. mellonella model, indicating that it is a biocompatible and safe materialpt_BR
dc.identifier.citationBRASIL, Giovana Sant’Ana Pegorin et al. Natural latex serum: characterization and biocompatibility assessment using galleria mellonella as an alternative in vivo model. Journal of Biomaterials Science, Polymer Edition, [s. l.], p. 1-22, 30 dez. 2021. Disponível em: https://www.tandfonline.com/doi/full/10.1080/09205063.2021.2014027. Acesso em: 29 mar. 2022. http://dx.doi.org/10.1080/09205063.2021.2014027pt_BR
dc.identifier.doi10.1080/09205063.2021.2014027
dc.identifier.issn1568-5624
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/46766
dc.languageenpt_BR
dc.publisherTaylor and Francispt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectNatural latex serumpt_BR
dc.subjectHevea brasiliensispt_BR
dc.subjectWestern blotpt_BR
dc.subjectHPLCpt_BR
dc.subjectToxicitypt_BR
dc.subjectGalleria mellonellapt_BR
dc.titleNatural latex serum: characterization and biocompatibility assessment using galleria mellonella as an alternative in vivo modelpt_BR
dc.typearticlept_BR

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