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Artigo Um laboratório a céu aberto: educação para biodiversidade na formação em nutrição com uso do método da aprendizagem baseada em hortas(Desenvolvimento e Meio Ambiente, 2021-05-27) Mota, Ana Carolina Costa Campos; Gomes,Ana Francisca Teixeira; Porciúncula, Laura Brito; Chaves, Viviany Moura; Almeida, Adriana Monteiro de; Jorge, Thiago Perez; Jacob, Michelle Cristine Medeiros; https://orcid.org/0000-0001-5503-0096; https://orcid.org/0000-0003-1265-0089; https://orcid.org/0000-0002-9654-7152; https://orcid.org/0000-0001-5447-8593; https://orcid.org/0000-0002-0375-8613; https://orcid.org/0000-0002-4881-7285No campo da nutrição, há uma demanda crescente por recursos humanos com capacidade para promover dietas sustentáveis. A ciência da nutrição, no entanto, enfrenta desafios que limitam sua capacidade de criar uma força de trabalho adequada para responder a essa demanda. A falta de conhecimento sobre biodiversidade, central para as dietas sustentáveis, é uma delas. Essa deficiência é parcialmente causada por tradições fragmentadas de pensamento – social, ambiental e biológico – na área. Argumentamos que a educação para biodiversidade, com o apoio do método de Aprendizagem Baseada em Hortas (ABH), podem ajudar a abordar esse limite. O estudo é um relato de experiência de professores e alunos envolvidos em um laboratório horta em um curso de nutrição de uma universidade pública do nordeste do Brasil. Descrevemos o histórico, o design e a metodologia de trabalho do laboratório, focada no tema de Plantas Alimentícias Não Convencionais. Apresentamos exemplos da aplicação do método a partir de experiências com plantas estudadas em 2019.O uso do método ABH proporcionou oportunidades para ação objetivando o enfrentamento dos desafios apresentados pela educação para biodiversidade: discussão sobre a formação dos professores, institucionalização do laboratório, inclusão de um curso sobre Sistemas alimentares sustentáveis no currículo, trabalho com uma equipe multiprofissional, fortalecimento dos laços comunitários e adaptação de ferramentas para contextos transculturais. Nessas ações, abordamos sistematicamente o conhecimento sobre taxonomia, filogenia, ecossistemas e diversidade cultural. A escassez de literatura sobre o assunto limitou nossa capacidade de analisar nosso trabalho dentro de um quadro prévio de referências. Esperamos que nosso relato possa incentivar iniciativas semelhantes, orientando o uso do método. Em pesquisas futuras, pretendemos medir o impacto dessas estratégias no desenvolvimento de competências para trabalho com a biodiversidade na formação em nutriçãoArtigo Aplicativo móvel como ferramenta de integração e promoção de serviços na Biblioteca Setorial da Escola Multicampi de Ciências Médicas do Rio Grande do Norte(Cenas Educacionais, 2025-04-09) Cunha Neto, José da; Bezerra, Lehi Aguiar; Varela, Marcelo da Silva; Viana, Marcos Paulo Barros; Albuquerque Neto, Aurélio Gomes de; Amorim, Érico Gurgel; Braz, Janine Karla França da SilvaAs bibliotecas acadêmicas têm adotado o formato digital/eletrônico em resposta às demandas crescentes da sociedade tecnológica. A Biblioteca Setorial Dr. Paulo Bezerra da Escola Multicampi de Ciências Médicas do Rio Grande do Norte oferece uma variedade de produtos e serviços à comunidade acadêmica e externa. No entanto, enfrenta desafios em promover e dar visibilidade a esses recursos. Este estudo teve como objetivo desenvolver um aplicativo móvel para facilitar o acesso e ampliar a visibilidade dos produtos e serviços da biblioteca, promovendo uma interação mais eficiente com os usuários. A pesquisa teve caráter exploratório, aplicado e qualitativo, sendo conduzida como um estudo de caso. O aplicativo foi desenvolvido por meio do Glide Apps, incluindo recursos como busca de informações e canais de comunicação direta. A avaliação do aplicativo, realizada com 52 participantes, destacou sua praticidade, intuitividade e relevância como ferramenta de integração acadêmica. Conclui-se que o aplicativo atende às necessidades informacionais da comunidade, potencializando a visibilidade e a utilização dos serviços da biblioteca.Artigo Estudo comparativo da atuação do enfermeiro no controle de dengue e febre chikungunya(FapUNIFESP (SciELO), 2016) Almeida Júnior, José Jailson de; Oliveira, Franklin Learcton Bezerra de; Millions, Rejane Medeiros; Costa, Marcelo Viana da; Silva, Dany Geraldo Kramer Cavalcanti eO objetivo deste estudo foi analisar a atuação dos enfermeiros das Estratégias de Saúde da Família no controle de dengue e febre chikungunya nos municípios de Parnamirim e Santa Cruz/RN. Trata-se de um estudo exploratório, descritivo, com abordagem qualitativa de tratamento e análise de dados. A coleta foi realizada entre novembro e dezembro de 2015 por meio de uma entrevista gravada, voltada às questões que abordam a ação da enfermagem e sua equipe no controle de dengue e febre chikungunya no campo de atuação da ESF. Utilizou-se como método de observação dos dados a análise de conteúdo de Bardin. Após análise, houve criação de duas categorias – educação em saúde e campanhas pontuais. Na primeira, os entrevistados informaram realizar palestras educativas, mas não explicam a metodologia usada e nem a participação popular, como também a articulação com o setor de endemias da cidade para fortalecer a discussão. Na segunda, algumas ações são assistencialistas de caráter campanhista/higienista, realizadas pelos enfermeiros dos municípios em campanhas de “higienização” e “limpeza”, as quais se mostram bastante presentes nas falas dos entrevistados. Há uma forte presença desse modelo enraizada tanto nas ações quanto na realização de atividades educativas, usando como metodologia a palestra. Além disso, há necessidade de se realizar mais estudos que aprofundem o tema abordado, a julgar por uma amostra pequena, especialmente no município de ParnamirimArtigo Can people self-select an exercise intensity sufficient to enhance muscular strength during weight training? A systematic review protocol of intervention studies(Medicine, 2019) Piuvezam, Grasiela; Segundo, Victor Hugo de Oliveira; Azevedo, Kesley Pablo Morais de; Medeiros, Humberto Jefferson de; Leitão, José Carlos; Knackfuss, Maria Irany; https://orcid.org/0000-0002-2343-7251Background: Previous studies have reported that self-selection of the training intensity can be an interesting strategy to improve adherence in aerobic exercise programs. However, its effectiveness with weight training has not been systematically reviewed and remains unclear. In this study, we will describe a systematic review protocol that aims to investigate if people are able to self-select an intensity during weight training sufficient to enhance muscular strength. Methods: This protocol is guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols. In this study, we will search the following electronic databases: PubMed, Science Direct, Scopus, Web of Science and SPORTDiscus. Intervention studies with at least one weight training session performed at self-selected intensity, with people from both genders and all age ranges will be included. The Downs & Black checklist will be used for methodological quality assessment. Two experienced reviewers will independently perform the selection of studies, data extraction, and evaluation of the methodological quality. Conclusion: This will be the first systematic review describing the results of weight training intervention studies with self-selected intensity. This study will provide high-quality and reliable evidence for health professionals and may direct methodological recommendations for further studiesArtigo Red meat consumption, risk of incidence of cardiovascular disease and cardiovascular mortality, and the dose–response effect: protocol for a systematic review and meta-analysis of longitudinal cohort studies(Medicine, 2019) Piuvezam, Grasiela; Medeiros, Gidyenne Christine Bandeira Silva de; Azevedo, Kesley Pablo Morais de; Mesquita, Gabriella Xavier Barbalho; Lima, Severina Carla Vieira Cunha; Silva, David Franciole de Oliveira; Pimenta, Isac Davidson Santiago Fernandes; Gonçalves, Ana Katherine da Silveira; Lyra, Clélia de Oliveira; https://orcid.org/0000-0002-2343-7251Background: Red and (particularly) processed meats are high in cholesterol and saturated and solid fatty acids. Their consumption is considered one of the risk factors for metabolic disorders. Numerous studies demonstrated a possible association between red meat consumption and cardiovascular disease (CVD). In this protocol, we propose a systematic review of the literature to examine the associations of red meat consumption with CVD incidence and mortality, and explore the potential dose–response relationship. Methods: We will search MEDLINE/PubMed, Scopus, SciELO, LILACS, ScienceDirect, Web of Science, Cochrane (CENTRAL), WHOLIS, PAHO, and Embase. We will include prospective epidemiological studies (longitudinal cohort). Risk of bias will be assessed using the Newcastle-Ottawa scale (NOS). Four independent researchers will conduct all evaluations. Disagreements will be referred to a fifth reviewer. We will summarize our findings using a narrative approach and tables to describe the characteristics of the included studies. The heterogeneity between trial results will be evaluated using a standard chi-squared test with P<.05. We will conduct the study in accordance with the guideline of the Preferred Reporting Items for Systematic Review and Meta-analyses Protocols (PRISMA-P). Results: This review will evaluate the association between red meat consumption and incidence of CVD and mortality (primary outcome measures). The secondary outcome measure will include the dose–response effect. Conclusion: The findings of this systematic review will summarize the latest evidence of the association between red meat consumption and incidence of CVD and mortality and the dose–response effect through a systematic review and meta-analysisArtigo Rituximab in systemic sclerosis: a protocol for systematic review(Medicine, 2019) Piuvezam, Grasiela; Caldas, Marina Maria Vieira de Figueiredo; Bezerra Neto, Francisco Alves; Azevedo, Kesley Pablo Morais de; Pimenta, Isac Davidson Santiago Fernandes; Oliveira, Ana Katherine da Silveira Gonçalves de; https://orcid.org/0000-0002-2343-7251Background: Systemic sclerosis (SSc) is a clinically complex and challenging disease, the most frequent complication of which is interstitial lung disease, which leads to a worse prognosis. In this situation, cyclophosphamide is considered the criterion standard for treatment, despite the controversies regarding its efficacy and toxicity. However, studies using rituximab (RTX) have shown that this drug may be a promising therapeutic option. The objective is to describe a protocol of a systematic review (SR) that analyzes the scientific evidence on the effects of RTX on SSc. Methods: This protocol is guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols. The databases to be searched are PubMed, Scopus, SciELO, LILACS, ScienceDirect, Web of Science, COCHRANE, WHOLIS, PAHO, and EMBASE. The studies that would be included in SR are clinical trials that evaluate the use of RTX in patients with SSc who meet the classification criteria for the disease according to American College of Rheumatology and European League Against Rheumatism (2013) and/or LeRoy criteria will be included in the SR. The data to be extracted are related to the characteristics of the studies: authors, year of publication, study location, type of study, sample size and age, patient characteristics, duration of intervention, therapeutic scheme, follow-up time, main variables, and main results. Results: In our study, we hope to find articles presenting new evidence supporting treatment of SSc with RTX. Conclusions: The SR will present results of scientific evidence for the effects of RTX in SSc. We hope that the results could strengthen clinical decisions for the best treatment of SSc and guide future researchesArtigo Isolamento de pacientes por coorte na pandemia de Coronavírus(Journal of Nursing and Health, 2020) Piuvezam, Grasiela; Silva, Tâmela Beatriz Matinada da; Sasso, Márcia Amaral Dal; Cunha, Leili Mara Mateus da; Silva, Gabriela de Oliveira; Bezerra, Isaac Newton Machado; https://orcid.org/0000-0002-2343-7251Objetivo: descrever a experiência de elaboração de Guia sobre isolamento em coorte de pacientes no contexto da pandemia de Coronavírus em uma rede de hospitais públicos de ensino brasileiros. Método: relato de experiência, baseado na observação da construção de Guia sobre isolamento em coorte nos hospitais, desenvolvido durante os meses de julho e agosto de 2020 pelas equipes das Unidades de Vigilância em Saúde, Segurança do Paciente e Gestão da Qualidade dos hospitais, durante a pandemia. Resultados: descreveu-se o processo de elaboração do Guia, apresentado segundo espaços de inovação e as 14 seções relacionadas à coorte de pacientes, baseadas nas recomendações vigentes. Conclusões: este relato de experiência poderá subsidiar outras instituições na adequação dos fluxos dos pacientes isolados por coorte de Coronavírus de forma segura e efetivaArtigo Placebo use and outcome quality: a protocol for systematic review and meta-analysis(Medicine, 2020) Piuvezam, Grasiela; Silva, Juliana Carvalho Rocha Alves da; Azevedo, Kesley Pablo Morais de; Farinasso, Cecília Menezes; Silva, Dayde Lane Mendonça da; Stefani, Cristine; Figueiredo, Ana Cláudia Morais Godoy; Souza, Patrícia Medeiros de; Capucho, Helaine Carneiro; https://orcid.org/0000-0002-2343-7251Background: The Pharmaceutical industry sponsorship, research outcome and quality has been already evaluated for clinical trials in order to analyze if this kind of sponsorship affects the results of clinical trials. In this sense, this study has the aim to investigate whether placebo use allows positive outcomes regarding efficacy and safety compared to synthetic medicines. Methods: We designed and registered a study protocol for a systematic review for methodology data. We will only randomized clinical trials that use placebo as comparator. The main outcome will be the evaluation of placebo use regarding the tendency for positive results (efficacy and security) when comparing to synthetic medicines. PubMed, Cochrane, LILACS (BVS), Web of Science, Scopus, and Excerpta Medica dataBASE (EMBASE) databases will be searched. Gray literature will be identified through the databases Proquest (Dissertation and Theses), OpenGrey and Google Scholar. Two review authors will independently assess trial quality and will extract data in accordance with standard Cochrane methodology. If necessary, we will also contact authors for additional information. The Cochrane Collaboration’s risk of bias tool will be used. If feasible, it means homogenous data, we will conduct random effects meta-analysis. Subgroup analyses will be conducted for different justifications for placebo use and for studies sponsored/not sponsored by the pharmaceutical industry. Results: Our present findings will indicate the effects of placebo use as comparator regarding efficacy and safety of the oral synthetic medicines. Discussion: This systematic review will identify, summarize, and analyze if there is a trend for positive efficacy and safety results for synthetic medicines in clinical trials when compared with placebo and if the justification for placebo use is considered ethically acceptable. Systematic review registration: PROSPERO CRD42018110829 Abbreviation: RCTs = randomized controlled clinical trialsArtigo Is rituximab effective for systemic sclerosis? A systematic review and meta-analysis(Springer, 2021) Piuvezam, Grasiela; Caldas, Marina Maria Vieira de Figueiredo; Azevedo, Kesley Pablo Morais de; Nunes, Ana Clara de França; Oliveira, Victor Hugo de; Pimenta, Isac Davidson Santiago Fernandes; Araújo, Isabela Dantas Torres de; Bezerra Neto, Francisco Alves; Oliveira, Ana Katherine da Silveira Gonçalves de; https://orcid.org/0000-0002-2343-7251Background: Systemic sclerosis (SSc) is a clinically complex and challenging disease, that leads to skin fibrosis. Its most frequent complication is interstitial lung disease (ILD), which leads to a worse prognosis. In this situation, cyclophosphamide is considered the gold standard for its treatment, despite the controversies regarding its efficacy and toxicity. However, studies using rituximab (RTX) have shown that this drug may be a promising therapeutic option. Objectives: This paper objective was to analyze the scientific evidence on the RTX effects on SSc. Methods: A systematic review (SR) was performed including clinical trials (CTs) on the use of RTX in SSc, published up to May 2020. The studies were identified through systematic searches in bibliographic databases using a predefined search strategy. The following databases were used: PUBMED, SCOPUS, SCIELO, LILACS, SCIENCE DIRECT, WEB OF SCIENCE, COCHRANE, WHOLIS, PAHO and EMBASE. Also, a manual search was performed. The methodological quality of the studies was determined using Jadad scale, Risk of Bias Tool (RoB 2.0) and Risk of Bias in Non-Randomized Studies - of Interventions tool (ROBINS-I). A meta-analysis of the randomized CTs was performed, using Review Manager. Results: Ten CTs were included in this SR. Of these, three were randomized and seven were non-randomized. Five showed a statistically significant improvement in forced vital capacity (FVC) at some time during follow-up. Regarding the skin, eight studies showed statistically significant improvements according toa the modified Rodnan skin score. The meta-analysis found positive effects of RTX in SSc, with a statistical significance for lung disease. Conclusion: Rituximab is a promising strategy for the SSc-associated ILD and cutaneous fibrosis treatment. PROSPERO registration number: CRD42019132018Artigo Effect of school-based food and nutrition education interventions on the food consumption of adolescents: a systematic review and meta-analysis(International Journal Of Environmental Research And Public Health, 2022) Piuvezam, Grasiela; Medeiros, Gidyenne Christine Bandeira Silva de; Azevedo, Kesley Pablo Morais de; Garcia, Daniel; Segundo, Victor Hugo Oliveira; Mata, Ádala Nayana de Sousa; Fernandes, Anny Karoliny Pinheiro; Santos, Raquel Praxedes dos; Trindade, Débora Danielly Barros de Brito; Moreno, Isabel Morales; Martínez, Daniel Guillén; https://orcid.org/0000-0002-2343-7251The school is a favorable environment for the development of interventions to prevent obesity. The objective of this systematic review is to evaluate the effects of school-based food and nutrition education interventions on adolescent food consumption. The literature search was conducted on databases: MEDLINE/PubMed, Embase, Scopus, ERIC, Science Direct, Web of Science, Cochrane, LILACS, and ADOLEC. The following research strategies were focused on: population (adolescents), intervention (food and nutrition education), outcome (food consumption), and study design (clinical trial). The Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) statement guidelines were followed and all stages of this review were performed by two researchers and, when necessary, a third researcher resolved discrepancies. Included studies are randomized clinical trials (RCT). A total of 24 articles were included for review and 11 articles in meta-analysis. In the evaluation of the general effects, there was a significant effect (mean difference (MD) for fruit consumption (MD = 0.09, CI 0.05, 0.14) in serving/day; and for vegetables (MD = 0.59, IC 0.15, 1.03) at times/week. In the consumption of FV (fruits and vegetables), there was no significant effect (standardized mean difference (SMD) of interventions in their consumption (SMD = 0.00, 95% C1 −0.11, 0.11). The evidence available in this review and meta-analysis concludes that food and nutrition education interventions in schools presented favorable results in the food consumption of adolescents. Registered on the PROSPERO database (CRD42019116520)Artigo Nontechnical skills training in intensive care units: protocol for a systematic review and meta-analysis(Plos One, 2023) Piuvezam, Grasiela; Pimenta, Isac Davidson Santiago Fernandes; Mata, Adala Nayana de Sousa; Bezerra, Isaac Newton Machado; Capucho, Helaine Carneiro; Oliveira, Adriana Catarina de Souza; Perez, Paloma Echevarrıa; https://orcid.org/0000-0002-2343-7251This study is aimed at describing a protocol for a systematic review and meta-analysis to assess the effect of nontechnical skills training on the acquisition of knowledge, skills or attitudes, and changes in behavior at the workplace, of healthcare professionals working in intensive care units (ICUs), as well as the effect on outcomes at an organizational level. We will search for original studies in the PubMed/Medline, Scopus, Web of Science, Science Direct, EMBASE and PsycINFO databases. Studies with a clinical trial or quasi-experimental design will be included. Two reviewers will independently screen and assess the included studies, with any disagreements being resolved by a third reviewer. We will summarize the findings using a narrative approach and, if possible, conduct a quantitative synthesis (meta-analysis). We will conduct the protocol following the Preferred Reporting Items for Systematic Review and Meta-Analyses Protocols (PRISMA-P) guidelines. The review will summarize the current evidence on nontechnical skills training in ICUs, examining satisfaction with the training program, improvements in knowledge about nontechnical skills and the adoption of safety behaviors, as well as improvement in outcomes for the organization, such as mortality rates, length of stay and cost indicators. We expect that the systematic review could indicate effective strategies for training ICU professionals in nontechnical skills and also determine whether these strategies really improve the safety culture and professional knowledge and behaviors, as well as patient outcomes and safetyArtigo Incidence of depression and depressive symptoms and their predictive factors in community-dwelling older adults: a systematic review and meta-analysis protocol(Bmj Open, 2021) Souza, Dyego Leandro Bezerra de; Brasileiro, Lízie Emanuelle Eulálio; Paiva, Aílla Lorenna de Medeiros; Medeiros, Maria Yasmin Dantas de; Roig, Javier Jerez; https://orcid.org/0000-0001-8426-3120Introduction Faced with the continuous growth in the number of older people at a global level, some concerns are raised about the way people age. Health conditions such as depressive symptoms and depression have a direct or indirect impact on the quality of life of this population segment. The objective of this study is to verify the incidence of the various presentations of the depressive spectrum in the community-dwelling older population, as well as to analyse the predictive factors. Methods and analysis This systematic review and meta-analysis protocol follows the recommendation of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols. Searches will be conducted in PubMed, Web of Science, Scopus, Latin-American and Caribbean Literature on Health Sciences, Scientific Electronic Library Online and Cochrane databases, as well as grey literature. The search strategy involves terms related to ageing and the depressive spectrum found in observational studies. There will be no language restriction and the material included will be the ones whose publications took place until December 2020. Ethics and dissemination Formal ethical approval is not required on this research, since it only aims secondary data. After publishing the results in a scientifically supported journal, our findings may be disseminated to fill in the gaps and guide the production of more effective public policies directed at a more adequate care to the older population at a global level. The search process began in January 2021 and it is expected that all stages of the review will be completed by 30 November 2021Artigo Validation of the QualiPresc instrument for assessing the quality of drug prescription writing in primary health care(Plos One, 2022) Souza, Dyego Leandro Bezerra de; Batista, Almaria Mariz; Gama, Zenewton Andre da Silva; https://orcid.org/0000-0001-8426-3120Introduction: Adverse events related to drug prescriptions are the main patient safety issue in primary care; however there is a lack of validated instruments for assessing the quality of prescription writing, which covers the prescriber, the patient and the drug information. Objective: To develop and validate the QualiPresc instrument to assess and monitor the quality of drug prescriptions in primary care, accompanied by a self-instruction direction, with the goal of filling the gap in validated instruments to assess the quality of prescription writing. Methodology: A validation study conducted in a municipality in Northeastern Brazil, based on prescriptions prepared in January 2021 by physicians assigned to 18 Basic Health Units and filed in 6 distribution/dispensing units. Four steps were covered: 1) Analysis of content validity of each indicator (relevance and adequacy); 2) Analysis of reliability via intra and inter-rater agreement of each indicator; 3) Analysis of the utility of each indicator; 4) Construction and analysis of the reliability of a weighted composite indicator based on effectiveness and safety scores for each indicator. Results: Twenty-nine potential indicators were listed, but only 13 were approved for validity, reliability and usefulness. Twelve indicators were excluded because of validity (<90% validity index) and four because they were not useful in the context of the study. Three weighted composite indicators were tested, but only one was approved for reliability and usefulness. The validated instrument therefore contains 13 indicators and 1 weighted composite indicator. Conclusion: This study demonstrates the validity, reliability and usefulness of QualiPresc for the evaluation of prescription writing in the context of primary care. Application to contexts such as secondary care and tertiary care requires cross-cultural adaptation and new content validity. Educators, managers and health care professionals can access QualiPresc online, free of charge, to assess performance and provide feedback involving drug prescribersArtigo Mechanisms of action of molecules with anti-TNF alpha activity on intestinal barrier inflammation: a systematic review protocol(Medicine, 2019) Morais, Ana Heloneida de Araujo; Lima, Mayara Santa Rosa; Lima, Vanessa Cristina Oliveira de; Piuvezam, Grasiela; Azevedo, Kesley Pablo Morais de; Maciel, Bruna Leal Lima; https://orcid.org/0000-0002-6460-911XBackground: Tumor necrosis factor-alpha (TNF-alpha), among cytokines that mediate the inflammatory process, plays an important role in diseases involving the loss of intestinal barrier integrity. Several molecules with anti-TNF-alpha activity have been studied aiming to develop new therapies. The purpose of this paper is to describe the systematic review protocol of experimental studies that determine mechanisms of action of molecules with anti-TNF-alpha activity on intestinal barrier inflammation. Methods:This protocol is guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyzes Protocols (PRISMA P). The databases to be searched are PubMed, EMBASE, Scopus, ScienceDirect, and Web of Science. Experimental studies in rats or mice that assessed the activity of anti-TNF-alpha molecules in models of intestinal barrier inflammation will be included in the systematic review. Studies characteristics, experimental model, and main results will be described and the bias risk assessment will be performed. Two independent reviewers will perform study selection, data extraction, and methodological quality assessment. A narrative synthesis will be made for the included studies. Also, if sufficient data is available, a meta-analysis will be conducted.I2 statistics will be used to assess heterogeneity. Results: The present protocol will assist in producing a systematic review that identifies the mechanisms underlying the reduction of TNF-alpha in intestinal barrier inflammation models. Conclusion: The systematic review may contribute to the theoretical basis of research on new molecules with anti-TNF-alpha potential and, consequently, in the development of new therapies employed in humans.Artigo Quality of prescription writing in Brazilian primary health care(Primary Health Care Research & Development, 2023) Souza, Dyego Leandro Bezerra de; Batista, Almária Mariz; Gama, Zenewton André da Silva; Hernández, Pedro Jesús Saturno; https://orcid.org/0000-0001-8426-3120Objective: To evaluate the quality of prescription writing in the context of public primary health care. Background: Prescription errors are one of the leading patient safety problems in primary care and can be caused by errors in therapeutic decisions or in the quality of prescription writing. Methods: Cross-sectional observational study conducted in a municipality in Northeastern Brazil. The assessment instrument (including 13 indicators and one composite indicator) was applied to a representative sample of drug prescriptions from the 24 Family Health Teams providing Primary Health Care in the municipality, dispensed in January 2021. Estimates of compliance and their 95% confidence intervals and graphical analysis of frequencies are assessed globally and stratified by dispensing units and prescribers. Findings: The average composite prescription writing quality on a 0-100 scale was 60.2 (95% CI 57.8–62.6). No quality criteria had 100% compliance. The highest compliance rates were found for ‘frequency of administration’ (98.9%) and ‘identification of the prescriber’ (98.9%). On the other hand, ‘recorded information on allergy’ (0.0%), ‘patient’s date of birth’ (1.7%), ‘nonpharmacological recommendations’ (1.7%), and ‘guidance on the use of the drug’ (25%) were the indicators with lower compliance, contributing to 69% of the noncompliances found. The type and frequency of the errors in the quality of prescription writing uncovered in this study confirm the continuing need to tackle this problem to improve patient safety. The results identify priority aspects for interventions and further studies on the quality of prescription writing in the context of Primary Health Care in BrazilArtigo Lactobacillus rhamnosus interferes with candida albicans adherence and biofilm formation: a potential alternative treatment of candidiasis(Austin Publishing Group, 2022-05-01) Ribeiro, F. C.; Iglesias, M. C.; Barros, Patrícia Pimentel de; Santos, S. S. F.; Jorge, A. O. C.; Leão, M. P. V.; https://orcid.org/0000-0003-4885-2811The objective of the present study was to evaluate the ability of Lactobacillus rhamnosus, on different preparations (living lactobacilli, dead by heat lactobacilli and supernatant of lactobacilli suspension), to interfere with Candida albicans adherence to ephitelial cells and biofilm formation. The results showed a reduction of 66.2% in the number of Candida cells adhered to epithelial cells, when the suspension of living L. rhamnosus was used. On the same way, this suspension reduced the in vitro biofim formation by C. albicans. In conclusion, the suspension with living cells of L. rhamnosus was able to reduce the ability of C. albicans to adhere on ephitelial cells and to form biofilm, suggesting a potential use of this probiotic bacteria as a therapeutic agent in candidiasisArtigo Natural latex serum: characterization and biocompatibility assessment using galleria mellonella as an alternative in vivo model(Taylor and Francis, 2021-12-30) Brasil, Giovana Sant’Ana Pegorin; Barros, Patrícia Pimentel de; Miranda, Matheus Carlos Romeiro; Barros, Natan Roberto de; Junqueira, Juliana Campos; Gomez, Alejandro; Herculano, Rondinelli Donizetti; Mendonça, Ricardo José de; https://orcid.org/0000-0003-4885-2811Natural latex serum (NLS) is one of the natural rubber latex fractions from Hevea brasiliensis tree, which is formed by centrifuged serum and is composed of proteins, acids, nucleotides, salts and carbohydrates. The proteins present in NLS have demonstrated several interesting biological properties, including angiogenic, healing, osteogenic, anti-inflammatory, antimicrobial, in addition to inducing neovascularization, bone formation and osseointegra tion. Thus, we proposed to characterize NLS by physicochemical techniques and to investigate the biocompatibility by toxicological assays and safety test in Galleria mellonella. Infrared spectrum showed vibrational bands characteristic of amide I, II and III that are linked to the protein content, which was confirmed by the High Performance Liquid Chromatography profile and by the Electrophoresis analysis. This material did not exhibit hemolytic (rate <0.5%) and cytotoxic effects (viability >70%) and was able to enhance the proliferation of fibroblasts (>600%) after 3 days. The pronounced proliferative effect observed in fibroblast cells can be explained by the presence of the fibroblast growth factor (FGF) like protein revealed by the Western blot test. Moreover, NLS did not provoke toxic effects (survival ~ 80%) on the G. mellonella model, indicating that it is a biocompatible and safe materialArtigo The anti-biofilm efficacy of Caffeic Acid Phenethyl Ester (CAPE) in vitro and a murine model of oral candidiasis(Frontiers Media, 2021-08-02) Barros, Patrícia Pimentel de; Rossoni, Rodnei Dennis; Terra-Garcia, Maíra; Kaminski, Valéria de Lima; Loures, Flávio Vieira; Fuchs, Beth Burgwyn; Mylonakis, Eleftherios; Junqueira, Juliana Campos; https://orcid.org/0000-0003-4885-2811Candida albicans is the main fungal species associated with the development of oral candidiasis. Currently, therapeutic options for these infections are limited by the adverse effects of antifungal drugs and by the emergence of drug resistant strains. Thus, the development of new antifungal agents is needed for the prevention and treatment of oral Candida infections. Caffeic acid phenethyl ester (CAPE) is a natural compound from propolis polyphenolic groups that exhibits many pharmacological properties. In this study, we investigated whether CAPE can have antifungal and immunomodulatory effects on oral candidiasis. Preliminary tests to assess the antifungal activity of CAPE were performed using the Minimum Inhibitory Concentration (MIC) assay that demonstrated inhibition in a range from 16 to 32 mg/mL, confirming its antifungal activity on several C. albicans strains isolated from the oral cavity. Subsequently, we analyzed Candida spp biofilms formed in vitro, in which CAPE treatment at 5 x MIC caused a reduction of 68.5% in the total biomass and ~2.60 Log in the viable cell count (CFU/mL) in relation to the untreated biofilm (p<0.0001). Next, RNA was extracted from untreated and CAPE-treated biofilms and analyzed by real-time qPCR. A series of genes analyzed (ALS1, ECE1, EPA1, HWP1, YWP1, BCR1, BGR1, CPH1, EFG1, NDT80, ROB1, TEC1, UME6, SAP2, SAP5, PBL2, and LIP9) were downregulated by CAPE compared to the untreated control group (p<0.0001). In in vivo studies using Galleria mellonella, the treatment with CAPE prolonged survival of larvae infected by C. albicans by 44.5% (p < 0.05) and accompanied by a 2.07-fold increase in the number of hemocytes. Flow cytometry revealed the most prominent increases were in types P2 and P3 hemocytes, granular cells, which phagocytize pathogens. In addition, CAPE treatment decreased the fungal load in the hemolymph and stimulated the expression of antifungal peptide genes such as galiomicin and gallerimycin. The antifungal and immunomodulatory activities observed in G. mellonella were extended to a murine model of oral candidiasis, in which CAPE decreased the levels of C. albicans colonization (~2 log CFU/mL) in relation to the untreated control group. In addition, CAPE treatment significantly reduced pseudomembranous lesions, invasion of hyphae on epithelium surfaces, tissue damage and inflammatory infiltrate (p < 0.05). CAPE was also able to increase the expression of b-defensin 3 compared to the infected and untreated group by 3.91-fold (p < 0.0001). Taken together, these results show that CAPE has both antifungal and immunomodulatory effects, making it a promising natural antifungal agent for the treatment and prevention of candidiasis and shows impact to oral candidiasisArtigo Antimicrobial effects of photodynamic therapy with Fotoenticine on Streptococcus mutans isolated from dental caries(Elsevier, 2021-06) Terra-Garcia, Maíra; Souza, Cheyenne Marçal de; Gonçalves, Nathalia Maria Ferreira; Pereira, André Henrique Correia; Barros, Patrícia Pimentel de; Borges, Alessandra Bühler; Miyakawa, Walter; Strixino, Juliana Ferreira; Junqueira, Juliana Campos; https://orcid.org/0000-0003-4885-2811Photodynamic therapy (PDT) is a promising strategy to control cariogenic pathogens, such as Streptococcus mutans. Seeking to reach the total bacterial elimination from dental surfaces, novel photosensitizers have been investigated, such as Fotoenticine (FTC) derived from chlorin e6. The objective of this study was to investigate the photodynamic effects of FTC against several clinical strains of S. mutans. Clinical isolates were obtained from patients with active carious lesions, identified by molecular analysis and subjected to PDT using laser irradiation (660 nm and 39.5 J/cm2) in planktonic and biofilm stages. We identified 11 S. mutans strains from cervical, occlusal and proximal caries. PDT mediated by FTC has totally eliminated the S. mutans cells in planktonic growth for all analyzed strains. In biofilms, PDT with FTC reached statistically significant reductions compared with the non-treated control group, at 5.4, 5.5 and 6.5 Log10 (CFU/mL), respectively, for the strains from proximal, occlusal and cervical caries. The scanning electron microscopy evaluations confirmed that PDT mediated by FTC was able to disaggregate and kill the S. mutans cells adhered to enamel surface, suggesting its potential to disinfect the dental tissuesArtigo Self-esteem of high-risk pregnant women(Academe Research Journals, 2019-12) Silva, Liniker Scolfild Rodrigues da; Cordeiro, Eliana Lessa; Douberin, Cristina Albuquerque; Linhares, Francisca Márcia Pereira; Patriota, Adriane Farias; Pimenta, Clarissa Silva; Silva, Mariana Batista da; Andrade, Iara Alves Feitoza de; Santos, Fernanda Barbosa dos; Ferreira, Ianne Larisse Alves; Marinho, Francielly Karoliny Barbosa Dantas; Costa, Renato Willamy da SilvaObjective: To analyze the self-esteem of pregnant women admitted to the high-risk ward of a reference hospital in the city of Recife. Materials and Methods: This is a cross-sectional, descriptive study with a quantitative approach. The sample population included 112 pregnant women.Data collection was performed through a form adapted from the version in the Brazil of the Rosenberg Self-Esteem Scale (RSES), plus socioeconomic, obstetric and reproductive questions. The sample included pregnant women admitted to the high-risk sector, and excluded pregnant women under age and those who presented inability of understanding and/or verbalization to answer the form. The data analysis was performed by means of descriptive statistics and the results, presented as tables. Results: 72.3% had unsatisfactory self-esteem. Conclusion: The low self-esteem was elevated in high-risk pregnant women. There is need for updating/training health professionals from high-risk maternity hospitals to identify signs of symptoms of low self-esteem in this population with the purpose of offering a reception and a suitable care