On the photovoltaic effect in local field potential recordings

Mikulovic, Sanja; Pupe, Stefano; Peixoto, Helton Maia; Nascimento, George Carlos; Kulander, Klas; Tort, Adriano Bretanha Lopes; Leão, Richardson Naves

On the photovoltaic effect in local field potential recordings

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dc.contributor.author	Mikulovic, Sanja
dc.contributor.author	Pupe, Stefano
dc.contributor.author	Peixoto, Helton Maia
dc.contributor.author	Nascimento, George Carlos
dc.contributor.author	Kulander, Klas
dc.contributor.author	Tort, Adriano Bretanha Lopes
dc.contributor.author	Leão, Richardson Naves
dc.date.accessioned	2020-07-15T13:51:49Z
dc.date.available	2020-07-15T13:51:49Z
dc.date.issued	2016-01-19
dc.description.resumo	Optogenetics allows light activation of genetically defined cell populations and the study of their link to specific brain functions. While it is a powerful method that has revolutionized neuroscience in the last decade, the shortcomings of directly stimulating electrodes and living tissue with light have been poorly characterized. Here, we assessed the photovoltaic effects in local field potential (LFP) recordings of the mouse hippocampus. We found that light leads to several artifacts that resemble genuine LFP features in animals with no opsin expression, such as stereotyped peaks at the power spectrum, phase shifts across different recording channels, coupling between low and high oscillation frequencies, and sharp signal deflections that are detected as spikes. Further, we tested how light stimulation affected hippocampal LFP recordings in mice expressing channelrhodopsin 2 in parvalbumin neurons (PV/ChR2 mice). Genuine oscillatory activity at the frequency of light stimulation could not be separated from light-induced artifacts. In addition, light stimulation in PV/ChR2 mice led to an overall decrease in LFP power. Thus, genuine LFP changes caused by the stimulation of specific cell populations may be intermingled with spurious changes caused by photovoltaic effects. Our data suggest that care should be taken in the interpretation of electrophysiology experiments involving light stimulation	pt_BR
dc.identifier.citation	MIKULOVIC, S.; PUPE, S.; PEIXOTO, H.M.; NASCIMENTO, G.C.; KULLANDER, K.; TORT, A.B.L.; LEÃO, R.N.. On the photovoltaic effect in local field potential recordings. Neurophotonics, v. 3, p. 015002, 2016. Disponível em: https://www.spiedigitallibrary.org/journals/Neurophotonics/volume-3/issue-01/015002/On-the-photovoltaic-effect-in-local-field-potential-recordings/10.1117/1.NPh.3.1.015002.full. Acesso em: 14 jul. 2020. https://doi.org/10.1117/1.NPh.3.1.015002	pt_BR
dc.identifier.doi	10.1117/1.NPh.3.1.015002
dc.identifier.issn	2329-4248
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/29645
dc.language	en	pt_BR
dc.publisher	Society of Photo-optical Instrumentation Engineers	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Silicon	pt_BR
dc.subject	Solar cells	pt_BR
dc.subject	Photovoltaics	pt_BR
dc.subject	Electrodes	pt_BR
dc.subject	Modulation	pt_BR
dc.subject	Optogenetics	pt_BR
dc.subject	Remote sensing	pt_BR
dc.subject	Brain	pt_BR
dc.subject	Light	pt_BR
dc.subject	Neurons	pt_BR
dc.title	On the photovoltaic effect in local field potential recordings	pt_BR
dc.type	article	pt_BR