Avaliação macroscópica, bioquímica e comportamental do efeito de termogênicos em ratos submetidos à movimentação ortodôntica

Abstract

Introduction: Considering that caffeine increases metabolism, much has been questioned whether thermogenics based on this substance would also have some influence on the rate of orthodontic movement and rodent behavior. Objective: To evaluate the effect of two thermogenic brands on behavior, metabolism and orthodontic movement in rats. Methodology: This was an experimental, in vivo, randomized study, with descriptive and inferential basis. The sample consisted of 19 healthy male Wistar rats (Ratus norvegicus albinus) between 7 and 12 weeks of age, which were submitted to continuous orthodontic movement of the first permanent maxillary molars on the left side during the 21 day period. The rats were randomly divided into 3 groups: Control group (CG) - Rats submitted only to orthodontic movement and daily water intake (n = 7); Experimental group Thermogenic C4 (GT1) - Rats undergoing orthodontic movement and daily dose of thermogenic C4 (n = 8) and experimental Group Thermogenic PRE-HD (GT2) - Rats submitted to orthodontic movement and daily dose of thermogenic PRE-HD (n = 4). The analysis of orthodontic movement was done by obtaining the difference in distance (in mm) from the distal of the 1˚ molar left superior to the mesial of the 2˚ molar superior of the same side, a measurement performed in the cervical region with the aid of a tip compass dry; the systemic analysis was performed through biochemical and leukogram tests and the behavioral study through the open field test. Results: There was no statistically significant difference between groups GT1, GT2 and CG regarding the amount of orthodontic movement (p> 0.005). Regarding the open field test, there was a statistically significant difference (p <0.005) between the groups for the variables: ambulatory movements, standing movements, number of jumps, distance traveled, speed of distance traveled and movements performed in the center and on the edges.Regarding the systemic effects, the leukogram and the biochemical tests performed did not show significant results. Conclusion: The use of thermogenics has no influence on the rate of orthodontic movement in rats and does not produce relevant systemic changes, however, it is capable of affecting several aspects of the behavior of these animals.