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Título: Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms
Autor(es): Goto-Silva, Livia
Ayad, Nadia M. E.
Herzog, Iasmin L.
Silva, Nilton P.
Lamien, Bernard
Orlande, Helcio R. B.
Souza, Annie da Costa
Ribeiro, Sidarta
Martins, Michele
Domont, Gilberto B.
Junqueira, Magno
Tovar-Moll, Fernanda
Rehen, Stevens K.
Palavras-chave: Organoid cultures;brain organoids;computational fluid dynamics
Data do documento: 7-Mar-2019
Referência: GOTO-SILVA, L. et al. Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms. BMC Dev Biol., v. 19, n. 1, p. 3, mar. 2019. doi: 10.1186/s12861-019-0183-y
Resumo: BACKGROUND: Organoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids has not been determined. RESULTS: Here we used computational fluid dynamics (CFD) to simulate two multiplex culture conditions: steering plates on an orbital shaker and the use of a previously described bioreactor. The bioreactor had low speed and high shear stress regions that may affect cell aggregate growth, depending on volume, whereas the computed variables of the steering plates were closer to those of the spinning flask. CONCLUSION: Our protocol improves the initial steps of the standard brain organoid formation, and the produced organoids displayed regionalized brain structures, including retinal pigmented cells. Overall, we conclude that suspension culture on orbital steering plates is a cost-effective practical alternative to previously described platforms for the cultivation of brain organoids for research and multiplex testing.
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