Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/jspui/handle/123456789/29393
Title: Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation
Authors: Batistella, Luciane
Ustra, Mara K.
Richetti, Aline
Pergher, Sibele Berenice Castellã
Treichel, Helen
Oliveira, J. V.
Lerin, Lindomar
Oliveira, Débora de
Keywords: Lipases;Enzyme activity;Ultrasound system;Enzyme stability
Issue Date: Mar-2012
Publisher: Springer
Citation: BATISTELLA, Luciane; USTRA, Mara K.; RICHETTI, Aline; PERGHER, Sibele Berenice Castellã; TREICHEL, Helen; OLIVEIRA, J. V.; LERIN, Lindomar; OLIVEIRA, Débora de. Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation. Bioprocess And Biosystems Engineering, [s.l.], v. 35, n. 3, p. 351-358, mar. 2012. Disponível em: https://link.springer.com/article/10.1007/s00449-011-0573-4. Acesso em: 19 maio 2020. https://doi.org/10.1007/s00449-011-0573-4.
Portuguese Abstract: Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure, the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents.
URI: https://repositorio.ufrn.br/jspui/handle/123456789/29393
ISSN: 1615-7591 (print)
Appears in Collections:IQ - Artigos publicados em periódicos

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