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Título: | Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B |
Título(s) alternativo(s): | Nanoparticles of poly(hydroxybutyrate-co-hydroxyvalerate) as support for the immobilization of Candida antarctica lipase (fraction B) |
Autor(es): | Fernandes, Ilizandra A. Nyari, Nádia L. D. Oliveira, José Vladimir de Rigo, Elisandra Souza, Maria Cristiane M. de Gonçalves, Luciana R. B. Pergher, Sibele Berenice Castellã Oliveira, Débora de |
Palavras-chave: | Nanoparticles;Immobilization;Lipases |
Data do documento: | Abr-2014 |
Editor: | Sociedade Brasileira de Química |
Referência: | FERNANDES, I. A.; NYARI, N. L. D.; OLIVEIRA, J. V. de; RIGO, E.; SOUZA, M. C. M. de; GONÇALVES, L. R. B.; PERGHER, Sibele Berenice Castellã; OLIVEIRA, D. de..Nanopartículas de poli-hidroxibutirato-co-valerato como suporte para a imobilização da lipase de Candida antarctica fração B. Química Nova (Impresso), v. 37, n. 2, p. 331-336, abr. 2014. ISSN 0100-4042. Disponível em: http://static.sites.sbq.org.br/quimicanova.sbq.org.br/pdf/v37n2a22.pdf. Acesso em 25 maio 2020. http://dx.doi.org/10.5935/0100-4042.20140055 |
Resumo: | This work evaluates the immobilization of Candida antarctica lipase (Fraction B) using poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanoparticles as support. The effects of immobilization time (30-150 min) and pH (5-10) on lipase loading were evaluated. The stability of the immobilized enzyme towards temperature (40, 60, and 80 ºC), reuse and storage (at 4 ºC) were also determined. Furthermore, to assess its potential application in a system of interest, the immobilized lipase was used as a catalyst in the esterification of geraniol with oleic acid. The results indicated a time of 120 minutes and pH of 7 as optimal for immobilization. A 21 hour exposure of the PHBV-lipase derivative to 60 ºC showed a 33% reduction of the initial activity while storage at 4 ºC led to a residual activity (5% of the original activity). The derivative was used without significant loss of activity for 4 successive cycles. The use of the immobilized lipase as a catalyst in the production of geranyl oleate led to about 88% conversion of the initial reactants to products |
URI: | https://repositorio.ufrn.br/jspui/handle/123456789/29631 |
ISSN: | 0100-4042 |
Aparece nas coleções: | IQ - Artigos publicados em periódicos |
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Arquivo | Descrição | Tamanho | Formato | |
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NanopartículasPoli-hidroxibutirato-co-valerato_Pergher_2014.pdf | 498,44 kB | Adobe PDF | Visualizar/Abrir |
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