Novos curativos biodegradáveis com biopolímeros e triterpeno Alfa Beta-Amirenona: obtenção, caracterização e avaliação da atividade cicatrizante e anti-inflamatória in vivo

Abstract

α, β-amirenone (ABAME) is a mixture of two natural isomers that are part of the triterpene class, where they have great potential for anti-inflammatory and healing activity. Chitosan films are increasingly used for topical use because they allow a gradual release of the drug and have many advantages such as biodegradable, flexible, durable and resistant. Thus, the objective of this study is to develop, characterize and evaluate chitosan films incorporated with α, β-amyrenone, in addition to analyzing the anti-inflammatory and healing effect, aiming at topical application in the treatment of skin lesions. The ABAME systems were evaluated by the following techniques: FTIR, DR-X, DSC, TG and SEM. In the in vivo part, the animal model was mice, which were subjected to injuries on the back and followed for 14 days to assess the healing process of the injury. After euthanasia, quantification of cytokines, macroscopic analysis of the lesions and histological analysis were performed. Thermal analysis demonstrated sample incorporation and an increase in the thermal stability of ABAME. The FTIR spectra of the films demonstrated interactions occurring between ABAME and the chitosan matrix. XRD results showed peaks in halos, characteristic of amorphous compounds, indicating the amorphization of ABAME by chitosan. SEM images showed the prototype homogeneously incorporated into the chitosan matrix and with partial loss of the crystalline characteristic of pure ABAME. In vivo, it was possible to observe that therapy with films incorporated with ABAME is quite effective, since it has anti-inflammatory properties that help in the healing process of skin lesions, making it possible to evaluate this evolution macroscopically, histologically and with the cytokines measured in the present study. In this study, the factors analyzed suggest that the dressing of chitosan with ABAME obtained a regenerating effect in mice, minimizing the parameters of the inflammatory process and progressing healing, thus validating its effectiveness. stages such as its use in humans with the purpose of elucidating its therapeutic applicability.