D2 dopamine receptor regulation of learning, sleep and plasticity

dc.contributor.authorFrança, A.S.C.
dc.contributor.authorSoares, Bruno Lobão
dc.contributor.authorNascimento, George Carlos do
dc.contributor.authorJeronimo, Selma Maria Bezerra
dc.contributor.authorRibeiro, Sidarta Tollendal Gomes
dc.date.accessioned2015-04-14T13:28:41Z
dc.date.available2015-04-14T13:28:41Z
dc.date.issued2015-01-16
dc.description.abstractDopamine and sleep have been independently linked with hippocampus-dependent learning. Since D2 dopaminergic transmission is required for the occurrence of rapid-eye-movement (REM) sleep, it is possible that dopamine affects learning by way of changes in post-acquisition REM sleep. To investigate this hypothesis, we first assessed whether D2 dopaminergic modulation in mice affects novel object preference, a hippocampus-dependent task. Animals trained in the dark period, when sleep is reduced, did not improve significantly in performance when tested 24 h after training. In contrast, animals trained in the sleep-rich light period showed significant learning after 24 h. When injected with the D2 inverse agonist haloperidol immediately after the exploration of novel objects, animals trained in the light period showed reduced novelty preference upon retesting 24 h later. Next we investigated whether haloperidol affected the protein levels of plasticity factors shown to be up-regulated in an experience-dependent manner during REM sleep. Haloperidol decreased post-exploration hippocampal protein levels at 3 h, 6 h and 12 h for phosphorylated Ca2+/calmodulin-dependent protein kinase II, at 6 h for Zif-268; and at 12 h for the brain-derived neurotrophic factor. Electrophysiological and kinematic recordings showed a significant decrease in the amount of REM sleep following haloperidol injection, while slow-wave sleep remained unaltered. Importantly, REM sleep decrease across animals was strongly correlated with deficits in novelty preference (Rho=0.56, p=0.012). Altogether, the results suggest that the dopaminergic regulation of REM sleep affects learning by modulating post-training levels of calcium-dependent plasticity factors.pt_BR
dc.description.sponsorshipe Pew Latin American Fellows Program in the Biomedical Sciences, Financiadora de Estudos e Projetos (FINEP) Grant 01.06.1092.00, Ministério da Ciência, Tecnologia e Inovação (MCTI), CNPq Universal 481351/2011-6, PQ 306604/2012-4, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), FAPERN/CNPq Pronem 003/2011, Capes SticAmSud, FAPESP Center for Neuromathematics (Grant no. 2013/07699-0, São Paulo Research Foundation), Associação Alberto Santos Dumont para Apoio à Pesquisa (AASDAP), and NIMBIOS working group “Multi-scale analysis of cortical networks.”pt_BR
dc.identifier.citationFrança AS, Lobão-Soares B, Muratori L, Nascimento G, Winne J, Pereira CM, Jeronimo SM, Ribeiro S.(2015). D2 dopamine receptor regulation of learning, sleep and plasticity. Neuropsychopharmacol. 2015 Jan 24. pii: S0924-977X(15)00012-7. doi: 10.1016/j.euroneuro.2015.01.011.pt_BR
dc.identifier.issn0924-977X
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/19009
dc.language.isoen_USpt_BR
dc.publisherElsevierpt_BR
dc.subjectREM sleeppt_BR
dc.subjectSono REMpt_BR
dc.subjectCaMKIIpt_BR
dc.subjectZif-268pt_BR
dc.subjectBDNFpt_BR
dc.subjectHaloperidolpt_BR
dc.subjectObject recognitionpt_BR
dc.subjectReconhecimento de objetospt_BR
dc.titleD2 dopamine receptor regulation of learning, sleep and plasticitypt_BR
dc.typearticlept_BR

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
D2DopamineReceptorRegulation_Franca_2015.pdf
Tamanho:
4.35 MB
Formato:
Adobe Portable Document Format
Descrição:
Artigo completo
Nenhuma Miniatura disponível
Baixar

Licença do Pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.53 KB
Formato:
Item-specific license agreed upon to submission
Nenhuma Miniatura disponível
Baixar