A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

Pinheiro, Thuane de Sousa; Santos, Marilia da S. Nascimento; Castro, Luiza Sheyla E. P. Will; Paiva, Almino Afonso de O.; Alves, Luciana G.; Cruz, Ana Katarina M.; Nobre, Leonardo Thiago Duarte Barreto; Alves, Monique Gabriela das Chagas Faustino; Leite, Edda Lisboa

A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line

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dc.contributor.author	Pinheiro, Thuane de Sousa
dc.contributor.author	Santos, Marilia da S. Nascimento
dc.contributor.author	Castro, Luiza Sheyla E. P. Will
dc.contributor.author	Paiva, Almino Afonso de O.
dc.contributor.author	Alves, Luciana G.
dc.contributor.author	Cruz, Ana Katarina M.
dc.contributor.author	Nobre, Leonardo Thiago Duarte Barreto
dc.contributor.author	Alves, Monique Gabriela das Chagas Faustino
dc.contributor.author	Leite, Edda Lisboa
dc.date.accessioned	2021-01-05T11:37:42Z
dc.date.available	2021-01-05T11:37:42Z
dc.date.issued	2017-02-19
dc.description.resumo	Polysaccharides from algae are also proper candidates with therapeutic properties and immunomodulatory and antitumor effects. The brown seaweed Lobophora variegata synthesizes different groups of anionic polysaccharides with several biological properties. Sulfated polysaccharides were obtained by delipidation of seaweed, proteolysis, and fractionation with different volumes of acetone. A fraction of sulfated polysaccharides, fucans or fucoidans, was extracted with 0.8 v acetone and named L. variegata (LV). This fucan was assessed in the inflammatory process in rats, antitumor action on human colon adenocarcinoma (HT-29 cells), apoptosis, and its effect on the cell cycle. LV fraction, a galactofucan, exhibited a high ratio of total sugar/sulfate (1.5) and a very low level of proteins. This polysaccharide showed an antiinflammatory effect on two models of inflammation induced by croton oil and oxazolone in rats. LV was analyzed in cellular proliferation of HT-29. We also demonstrated the cytotoxic action against this cell line and induction in the apoptosis and decreased the cell cycle in phases S and G2/M and the accumulation of cells in the G1 phase. Our studies with LV showed a marked immunomodulatory action without and with lipopolysaccharide (LPS) on RAW 264.7 cells. Comparative studies of two fucans, LV and FV (Fucus vesiculosus), with different structures were assessed on viability in macrophages, RAW 264.7 cells. Both showed cytotoxicity in these cells. We observed high levels of nitric oxide (NO) production, interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) when treated at 0.25, 0.5, and 1.0 mg mL−1 of LV. Data also suggest that LV has potential antitumor effects on HT-29 and antiinflammatory and immunomodulatory effects on RAW 264.7 cells	pt_BR
dc.identifier.citation	PINHEIRO, Thuane de Sousa; SANTOS, Marilia da S. Nascimento; CASTRO, Luiza Sheyla E. P. Will; PAIVA, Almino Afonso de O.; ALVES, Luciana G.; CRUZ, Ana Katarina M.; NOBRE, Leonardo Thiago Duarte Barreto; ALVES, Monique Gabriela das Chagas Faustino; LEITE, Edda Lisboa. A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line. Journal Of Applied Phycology, [s. l.], v. 29, n. 4, p. 2061-2075, 19 fev. 2017. Springer Science and Business Media LLC. Disponível em: https://link.springer.com/article/10.1007/s10811-017-1075-9. Acesso em: 21 set. 2020. http://dx.doi.org/10.1007/s10811-017-1075-9.	pt_BR
dc.identifier.doi	10.1007/s10811-017-1075-9
dc.identifier.issn	0921-8971 (print)
dc.identifier.issn	1573-5176 (online)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31172
dc.language	en	pt_BR
dc.publisher	Springer	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Sulfated polysaccharides	pt_BR
dc.subject	Antitumoral effect	pt_BR
dc.subject	Cytotoxicity	pt_BR
dc.subject	Lobophora variegata	pt_BR
dc.subject	Phaeophyceae	pt_BR
dc.subject	Apoptosis	pt_BR
dc.subject	DTH	pt_BR
dc.title	A fucan of a brown seaweed and its antitumoral property on HT-29 and immunomodulatory activity in murine RAW 264.7 macrophage cell line	pt_BR
dc.type	article	pt_BR