The protective role of sulfated polysaccharides from green seaweed Udotea flabellum in cells exposed to oxidative damage

Presa, Fernando Bastos; Marques, Maxsuell Lucas Mendes; Viana, Rony Lucas Silva; Nobre, Leonardo Thiago Duarte Barreto; Costa, Leandro Silva; Rocha, Hugo Alexandre de Oliveira

The protective role of sulfated polysaccharides from green seaweed Udotea flabellum in cells exposed to oxidative damage

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dc.contributor.author	Presa, Fernando Bastos
dc.contributor.author	Marques, Maxsuell Lucas Mendes
dc.contributor.author	Viana, Rony Lucas Silva
dc.contributor.author	Nobre, Leonardo Thiago Duarte Barreto
dc.contributor.author	Costa, Leandro Silva
dc.contributor.author	Rocha, Hugo Alexandre de Oliveira
dc.date.accessioned	2020-12-30T13:45:47Z
dc.date.available	2020-12-30T13:45:47Z
dc.date.issued	2018-04-20
dc.description.resumo	Seaweed is a rich source of bioactive sulfated polysaccharides. We obtained six sulfated polysaccharide-rich fractions (UF-0.3, UF-0.5, UF-0.6, UF-0.7, UF-1.0, and UF-2.0) from the green seaweed Udotea flabellum (UF) by proteolytic digestion followed by sequential acetone precipitation. Biochemical analysis of these fractions showed that they were enriched with sulfated galactans. The viability and proliferative capacity of 3T3 fibroblasts exposed to FeSO4 (2 µM), CuSO4 (1 µM) or ascorbate (2 mM) was not affected. However, these cells were exposed to oxidative stress in the presence of FeSO4 or CuSO4 and ascorbate, which caused the activation of caspase-3 and caspase-9, resulting in apoptosis of the cells. We also observed increased lipid peroxidation, evaluated by the detection of malondialdehyde and decreased glutathione and superoxide dismutase levels. Treating the cells with the ultrafiltrate fractions (UF) fractions protected the cells from the oxidative damage caused by the two salts and ascorbate. The most effective protection against the oxidative damage caused by iron was provided by UF-0.7 (1.0 mg/mL); on treatment with UF-0.7, cell viability was 55%. In the case of copper, cell viability on treatment with UF-0.7 was ~80%, but the most effective fraction in this model was UF-2.0, with cell viability of more than 90%. The fractions, mainly UF-0.7 and UF-2.0, showed low iron chelating activity, but high copper chelating activity and total antioxidant capacity (TAC). These results suggested that some of their protective mechanisms stem from these properties	pt_BR
dc.identifier.citation	PRESA, Fernando; MARQUES, Maxsuell; VIANA, Rony; NOBRE, Leonardo Thiago Duarte Barreto; COSTA, Leandro; ROCHA, Hugo Alexandre de Oliveira. The protective role of sulfated polysaccharides from green seaweed Udotea flabellum in cells exposed to oxidative damage. Marine Drugs, [s. l.], v. 16, n. 4, p. 135-150, 20 abr. 2018. MDPI AG. Disponível em: https://www.mdpi.com/1660-3397/16/4/135. Acesso em: 16 set. 2020. http://dx.doi.org/10.3390/md16040135.	pt_BR
dc.identifier.doi	10.3390/md16040135
dc.identifier.issn	1660-3397 (online)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31160
dc.language	en	pt_BR
dc.publisher	MDPI	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Sulfated galactan	pt_BR
dc.subject	3T3 fibroblasts	pt_BR
dc.subject	Green seaweed	pt_BR
dc.title	The protective role of sulfated polysaccharides from green seaweed Udotea flabellum in cells exposed to oxidative damage	pt_BR
dc.type	article	pt_BR