Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties

Oliveira, Caio Fernando Ramalho de; Flores, Taylla Michelle de Oliveira; Cardoso, Marlon Henrique; Oshiro, Karen Garcia Nogueira; Russi, Raphael; França, Anderson Felipe Jácome de; Santos, Elizeu Antunes dos; Franco, Octávio Luiz; Oliveira, Adeliana Silva de; Migliolo, Ludovico

Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties

Página do item simplificado Estatísticas

dc.contributor.author	Oliveira, Caio Fernando Ramalho de
dc.contributor.author	Flores, Taylla Michelle de Oliveira
dc.contributor.author	Cardoso, Marlon Henrique
dc.contributor.author	Oshiro, Karen Garcia Nogueira
dc.contributor.author	Russi, Raphael
dc.contributor.author	França, Anderson Felipe Jácome de
dc.contributor.author	Santos, Elizeu Antunes dos
dc.contributor.author	Franco, Octávio Luiz
dc.contributor.author	Oliveira, Adeliana Silva de
dc.contributor.author	Migliolo, Ludovico
dc.date.accessioned	2021-01-29T12:37:28Z
dc.date.available	2021-01-29T12:37:28Z
dc.date.issued	2019-11-28
dc.description.resumo	The Indianmeal moth, Plodia interpunctella, is one of the most damaging pests of stored products. We investigated the insecticidal properties of ApKTI, a Kunitz trypsin inhibitor from Adenanthera pavonina seeds, against P. interpunctella larvae through bioassays with artificial diet. ApKTI-fed larvae showed reduction of up to 88% on larval weight and 75% in survival. Trypsin enzymes extracted from P. interpunctella larvae were inhibited by ApKTI, which also demonstrated capacity to bind to chitin. Kinetic studies revealed a non-competitive inhibition mechanism of ApKTI for trypsin, which were further corroborated by molecular docking studies. Furthermore, we have demonstrated that ApKTI exhibits a hydrophobic pocket near the reactive site loop probably involved in chitin interactions. Taken together, these data suggested that the insecticidal activity of ApKTI for P. interpunctella larvae involves a dual and promiscuous mechanisms biding to two completely different targets. Both processes might impair the P. interpunctella larval digestive process, leading to larvae death before reaching the pupal stage. Further studies are encouraged using ApKTI as a biotechnological tool to control insect pests in field conditions	pt_BR
dc.identifier.citation	OLIVEIRA, Caio Fernando Ramalho de; FLORES, Taylla Michelle de Oliveira; CARDOSO, Marlon Henrique; OSHIRO, Karen Garcia Nogueira; RUSSI, Raphael; FRANÇA, Anderson Felipe Jácome de; SANTOS, Elizeu Antunes dos; FRANCO, Octávio Luiz; OLIVEIRA, Adeliana Silva de; MIGLIOLO, Ludovico. Dual insecticidal efects of Adenanthera pavonina kunitz-type inhibitor on Plodia interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties. Molecules, [s. l.], v. 24, n. 23, p. 4344-4360, 28 nov. 2019. Disponível em: https://www.mdpi.com/1420-3049/24/23/4344/htm. Acesso em: 26 jan. 2020. http://dx.doi.org/10.3390/molecules24234344	pt_BR
dc.identifier.doi	10.3390/molecules24234344
dc.identifier.issn	1420-3049
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31321
dc.language	en	pt_BR
dc.publisher	MDPI	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Non-competitive inhibitor	pt_BR
dc.subject	Trypsin inhibitor	pt_BR
dc.subject	Peritrophic membrane	pt_BR
dc.title	Dual insecticidal effects of Adenanthera Pavonina kunitz-type inhibitor on Plodia Interpunctella is mediated by digestive enzymes inhibition and chitin-binding properties	pt_BR
dc.type	article	pt_BR