Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium

Lucena, Eudes Euler de Souza; Morais, Hécio Henrique Araújo de; Araújo, Dayane Pessoa de; Cavalcanti, José Rodolfo Lopes de Paiva; Azevedo, Eduardo Pereira de; Queiroz, Dinalva Brito de; Botelho, Marco Antônio; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Barboza, Carlos Augusto Galvão; Nascimento Júnior, Expedito Silva do; Costa, Miriam Stela Maris de Oliveira; Cavalcante, Jeferson de Sousa; Guzen, Fausto Pierdoná

Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium

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dc.contributor.author	Lucena, Eudes Euler de Souza
dc.contributor.author	Morais, Hécio Henrique Araújo de
dc.contributor.author	Araújo, Dayane Pessoa de
dc.contributor.author	Cavalcanti, José Rodolfo Lopes de Paiva
dc.contributor.author	Azevedo, Eduardo Pereira de
dc.contributor.author	Queiroz, Dinalva Brito de
dc.contributor.author	Botelho, Marco Antônio
dc.contributor.author	Rêgo, Amália Cinthia Meneses do
dc.contributor.author	Araújo Filho, Irami
dc.contributor.author	Barboza, Carlos Augusto Galvão
dc.contributor.author	Nascimento Júnior, Expedito Silva do
dc.contributor.author	Costa, Miriam Stela Maris de Oliveira
dc.contributor.author	Cavalcante, Jeferson de Sousa
dc.contributor.author	Guzen, Fausto Pierdoná
dc.date.accessioned	2021-03-12T11:10:08Z
dc.date.available	2021-03-12T11:10:08Z
dc.date.issued	2018-09
dc.description.resumo	Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation	pt_BR
dc.identifier.citation	LUCENA, Eudes Euler de Souza et al. Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium. International Journal Of Morphology, [s. l.], v. 36, n. 3, p. 1049-1056, set. 2018. SciELO Agencia Nacional de Investigacion y Desarrollo (ANID). Disponível em: https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-95022018000301049&lng=pt&nrm=iso&tlng=en. Acesso em: 20 jul. 2020. http://dx.doi.org/10.4067/s0717-95022018000301049	pt_BR
dc.identifier.doi	10.4067/s0717-95022018000301049
dc.identifier.issn	0717-9367 (print)
dc.identifier.issn	0717-9502 (0nline)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31829
dc.language	en	pt_BR
dc.publisher	Sociedade Chilena de Anatomía	pt_BR
dc.rights	Attribution-NonCommercial 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc/3.0/br/	*
dc.subject	Mesenchymal stem cells	pt_BR
dc.subject	Facial nerve injury	pt_BR
dc.subject	Fibroblast growth factor 2	pt_BR
dc.subject	Conditioned medium	pt_BR
dc.subject	Plasticity	pt_BR
dc.subject	Trans-differentiation	pt_BR
dc.title	Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium	pt_BR
dc.title.alternative	Expansión y cambios fenotípicos de células mesenquimales de médula ósea de ratón cultivadas con FGF-2 y medio condicionado por el nervio facial	pt_BR
dc.type	article	pt_BR