Immunomodulatory effects and antimicrobial activity of heterofucans from Sargassum filipendula

Telles, Cinthia Beatrice Silva; Mendes-Aguiar, Carolina; Fidelis, Gabriel Pereira; Frasson, Amanda Piccoli; Pereira, Wogelsanger Oliveira; Scortecci, Katia Castanho; Camara, Rafael Barros Gomes; Nobre, Leonardo Thiago Duarte Barreto; Costa, Leandro Silva; Tasca, Tiana; Rocha, Hugo Alexandre de Oliveira

Immunomodulatory effects and antimicrobial activity of heterofucans from Sargassum filipendula

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dc.contributor.author	Telles, Cinthia Beatrice Silva
dc.contributor.author	Mendes-Aguiar, Carolina
dc.contributor.author	Fidelis, Gabriel Pereira
dc.contributor.author	Frasson, Amanda Piccoli
dc.contributor.author	Pereira, Wogelsanger Oliveira
dc.contributor.author	Scortecci, Katia Castanho
dc.contributor.author	Camara, Rafael Barros Gomes
dc.contributor.author	Nobre, Leonardo Thiago Duarte Barreto
dc.contributor.author	Costa, Leandro Silva
dc.contributor.author	Tasca, Tiana
dc.contributor.author	Rocha, Hugo Alexandre de Oliveira
dc.date.accessioned	2021-01-05T11:55:20Z
dc.date.available	2021-01-05T11:55:20Z
dc.date.issued	2017-07-18
dc.description.resumo	Parasitic diseases are a human health problem mainly in low-income areas. The drugs available for the treatment of these diseases are far from satisfactory due to high costs, toxicity, and drug resistance. Sulfated polysaccharides are a complex group of bioactive polymers and can be obtained from seaweeds. The heterofucans from Sargassum filipendula (SF) present strong antiproliferative and antioxidant activities. However, their immunomodulatory and antimicrobial capacity have not been evaluated until now. In this study, five sulfated fucose-rich fractions were isolated (named SF0.5V, SF0.7V, SF1.0V, SF1.5V, and SF2.0V). The chemical composition showed slight differences among polysaccharides and, consequently, biological activity of these polymers. Three fractions (SF0.5V, SF0.7V, and SF1.0V) showed a strong immunomodulatory activity enhancing the release of nitric oxide (NO) by murine macrophages (RAW 264.7), though only SF0.5V was able to induce interleukin-6 (IL-6) and TNF-α release from RAW cells. The sugar to sulfate ratio was not correlated with these activities. Meanwhile, the contents of xylose (P = 0.98 for NO; P = 0.98 for IL-6; P = 0.96 for TNF-α) and glucuronic acid (P = 0.91 for NO; P = 0.9190 for IL-6; P = 0.79 for TNF-α) were strongly positively correlated. SF0.7V and SF1.0V inhibited biofilm formation by Klebsiella pneumoniae (4.2 and 6.8%, respectively), whereas SF0.5V showed inhibitory effect (~50%) on biofilm formation by Staphylococcus epidermidis. Furthermore, SF0.7V and SF1.0V showed high inhibition capacity on the survival of the protozoan Trichomonas vaginalis. The sugar to sulfate ratio was positively correlated (P = 0.60) with this activity. The results demonstrate the spectrum of action of these sulfated polysaccharides obtained from SF and show their potential as immunomodulatory and microbicidal agents	pt_BR
dc.identifier.citation	TELLES, Cinthia Beatrice Silva; MENDES-AGUIAR, Carolina; FIDELIS, Gabriel Pereira; FRASSON, Amanda Piccoli; PEREIRA, Wogelsanger Oliveira; SCORTECCI, Katia Castanho; CAMARA, Rafael Barros Gomes; NOBRE, Leonardo Thiago Duarte Barreto; COSTA, Leandro Silva; TASCA, Tiana; ROCHA, Hugo Alexandre de Oliveira. Immunomodulatory effects and antimicrobial activity of heterofucans from Sargassum filipendula. Journal Of Applied Phycology, [s. l.], v. 30, n. 1, p. 569-578, 18 jul. 2017. Springer Science and Business Media LLC. Disponível em: https://link.springer.com/article/10.1007%2Fs10811-017-1218-z. Acesso em: 22 set. 2020. http://dx.doi.org/10.1007/s10811-017-1218-z.	pt_BR
dc.identifier.doi	10.1007/s10811-017-1218-z
dc.identifier.issn	0921-8971 (print)
dc.identifier.issn	1573-5176 (online)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31173
dc.language	en	pt_BR
dc.publisher	Springer	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Fucoidan	pt_BR
dc.subject	Trichomonas vaginalis	pt_BR
dc.subject	Antibiofilm	pt_BR
dc.subject	Nitric oxide	pt_BR
dc.title	Immunomodulatory effects and antimicrobial activity of heterofucans from Sargassum filipendula	pt_BR
dc.type	article	pt_BR