Discriminação de respostas metabólicas de Staphylococcus aureus submetidas a nanopartículas de prata

Abstract

Bacterial resistance is one of the problems faced by the pharmaceutical industry. Data from the literature show promising results in the application of silver nanoparticles (NanoAg) to combat the bacterial resistance, in addition to the possibility of combining them with drugs. In this study, the NanoAg were synthesized by a low environmental impact route. These nanoparticles and their combination with doxycycline hyclate (DO) were characterized with transmission electron microscopy (TEM) and UV-Vis spectroscopy (UV-Vis). Spectroscopy in the UV-vis region showed the formation of bands characteristic of NanoAg (401 nm), DO (273 nm and 346 nm). After the addition of DO to the NanoAg solution, the DO band shifted to 355nm due to interaction with NanoAg. TEM micrographs showed that the shape of the NanoAg was preserved after the addition of DO and promote a change in the mean particle size from 5.32 nm ± 1.02 nm to 4.32 nm ± 1.02 nm (p < 0, 05). Subsequently, the bacteria were activated, seeded, incubated and exposed to the antimicrobial agents (NanoAg, DO, NanoAg + DO), followed by the acquisition of their respective FTIR-ATR spectra. The Principal Component Analysis (PCA) applied to the group of spectra showed a pattern of differentiation between the classes.