Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae

Fidelis, Gabriel Pereira; Câmara, Rafael Barros Gomes da; Queiroz, Moacir Fernandes; Costa, Mariana Santana Santos Pereira; Santos, Pablo Castro; Rocha, Hugo Alexandre de Oliveira; Costa, Leandro Silva

Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae

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dc.contributor.author	Fidelis, Gabriel Pereira
dc.contributor.author	Câmara, Rafael Barros Gomes da
dc.contributor.author	Queiroz, Moacir Fernandes
dc.contributor.author	Costa, Mariana Santana Santos Pereira
dc.contributor.author	Santos, Pablo Castro
dc.contributor.author	Rocha, Hugo Alexandre de Oliveira
dc.contributor.author	Costa, Leandro Silva
dc.date.accessioned	2021-01-28T17:48:31Z
dc.date.available	2021-01-28T17:48:31Z
dc.date.issued	2014-11-13
dc.description.resumo	The sulfated polysaccharides (SP) from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1)-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g) increased in the following order: GB2sp > GB1sp > GB2s > GB1s > GB1. However, the amount of SP extracted increased in a different way: GB2sp > GB1 > GB1sp > GB1s > GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT) test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT) test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp > GB2sp > GB1 > GB1s > GB2s. The total capacity antioxidant (TCA) of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiae	pt_BR
dc.identifier.citation	FIDELIS, Gabriel Pereira; CAMARA, Rafael Barros Gomes da; QUEIROZ, Moacir Fernandes; COSTA, Mariana Santana Santos Pereira; SANTOS, Pablo Castro; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules, [s. l.], v. 19, n. 11, p. 18511-18526, 13 nov. 2014. MDPI AG. Disponível em: https://www.mdpi.com/1420-3049/19/11/18511. Acesso em: 05 ago. 2020. Http://dx.doi.org/10.3390/molecules191118511.	pt_BR
dc.identifier.doi	10.3390/molecules191118511
dc.identifier.issn	1420-3049 (online)
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/31315
dc.language	en	pt_BR
dc.publisher	MDPI	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	APTT assay	pt_BR
dc.subject	Total capacity antioxidant	pt_BR
dc.subject	Sonication	pt_BR
dc.subject	Proteolysis	pt_BR
dc.subject	Red seaweed	pt_BR
dc.title	Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae	pt_BR
dc.type	article	pt_BR