Aqueous leaf extract of Jatropha gossypiifolia L. (Euphorbiaceae) inhibits enzymatic and biological actions of Bothrops jararaca snake venom

dc.contributor.authorFélix-Silva, Juliana
dc.contributor.authorSouza, Thiago
dc.contributor.authorMenezes, Yamara A. S.
dc.contributor.authorCabral, Bárbara
dc.contributor.authorCâmara, Rafael Barros Gomes da
dc.contributor.authorSilva-Junior, Arnóbio Antonio da
dc.contributor.authorRocha, Hugo Alexandre de Oliveira
dc.contributor.authorRebecchi, Ivanise Marina Moretti
dc.contributor.authorLangassner, Silvana Maria Zucolotto
dc.contributor.authorFernandes-Pedrosa, Matheus de Freitas
dc.date.accessioned2021-01-18T12:21:59Z
dc.date.available2021-01-18T12:21:59Z
dc.date.issued2014-08-15
dc.description.resumoSnakebites are a serious public health problem due their high morbi-mortality. The main available specific treatment is the antivenom serum therapy, which has some disadvantages, such as poor neutralization of local effects, risk of immunological reactions, high cost and difficult access in some regions. In this context, the search for alternative therapies is relevant. Therefore, the aim of this study was to evaluate the antiophidic properties of Jatropha gossypiifolia, a medicinal plant used in folk medicine to treat snakebites. The aqueous leaf extract of the plant was prepared by decoction and phytochemical analysis revealed the presence of sugars, alkaloids, flavonoids, tannins, terpenes and/or steroids and proteins. The extract was able to inhibit enzymatic and biologic activities induced by Bothrops jararaca snake venom in vitro and in vivo. The blood incoagulability was efficiently inhibited by the extract by oral route. The hemorrhagic and edematogenic local effects were also inhibited, the former by up to 56% and the latter by 100%, in animals treated with extract by oral and intraperitoneal routes, respectively. The inhibition of myotoxic action of B. jararaca reached almost 100%. According to enzymatic tests performed, it is possible to suggest that the antiophidic activity may be due an inhibitory action upon snake venom metalloproteinases (SVMPs) and/or serine proteinases (SVSPs), including fibrinogenolytic enzymes, clotting factors activators and thrombin like enzymes (SVTLEs), as well upon catalytically inactive phospholipases A2 (Lys49 PLA2). Antiinflammatory activity, at least partially, could also be related to the inhibition of local effects. Additionally, protein precipitating and antioxidant activities may also be important features contributing to the activity presented. In conclusion, the results demonstrate the potential antiophidic activity of J. gossypiifolia extract, including its significant action upon local effects, suggesting that it may be used as a new source of bioactive molecules against bothropic venompt_BR
dc.identifier.citationFÉLIX-SILVA, Juliana; SOUZA, Thiago; MENEZES, Yamara A. S.; CABRAL, Bárbara; CÂMARA, Rafael Barros Gomes da; SILVA-JUNIOR, Arnóbio Antonio da; ROCHA, Hugo Alexandre de Oliveira; REBECCHI, Ivanise Marina Moretti; LANGASSNER, Silvana Maria Zucolotto; FERNANDES-PEDROSA, Matheus de Freitas. Aqueous leaf extract of Jatropha gossypiifolia L. (Euphorbiaceae) inhibits enzymatic and biological actions of Bothrops jararaca snake venom. Plos One, [s. l.], v. 9, n. 8, p. e104952, 15 ago. 2014. Public Library of Science (PLoS). Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104952. Acesso em: 05 ago. 2020. http://dx.doi.org/10.1371/journal.pone.0104952.pt_BR
dc.identifier.doi10.1371/journal.pone.0104952
dc.identifier.issn1932-6203 (online)
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/31269
dc.languageenpt_BR
dc.publisherPublic Library of Sciencept_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectAnticoagulantspt_BR
dc.subjectAntidotespt_BR
dc.subjectAntioxidantspt_BR
dc.subjectBlood Coagulationpt_BR
dc.subjectSnakebitespt_BR
dc.titleAqueous leaf extract of Jatropha gossypiifolia L. (Euphorbiaceae) inhibits enzymatic and biological actions of Bothrops jararaca snake venompt_BR
dc.typearticlept_BR

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