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Title: Effect of sildenafil in renal ischemia/reperfusion injury in rats
Other Titles: Efeito do sildenafil na lesão renal por isquemia-reperfusão em ratos
Authors: Medeiros, Paulo José de
Neto, Arthur Villarim
Lima, Francisco Pignataro
Azevedo, Ítalo Medeiros
Leão, Layra Ribeiro de Sousa
Medeiros, Aldo Cunha
Keywords: Ischemia;Reperfusion;Kidney;Radionuclide Imaging;Technetium Tc 99m Mertiatide;Rats
Issue Date: 2010
Citation: MEDEIROS, P. J. ; Villarim Neto, Arthur ; LIMA, Francisco Pignataro ; AZEVEDO, Ítalo Medeiros ; LEAO, L. R. S. ; MEDEIROS, A. C. . Effect of sildenafil in renal ischemia/reperfusion injury in rats. Acta Cirurgica Brasileira (Impresso) , v. 25, p. 490-495, 2010. Disponível em: <>. Acesso em:19 set.2013.
Abstract: To evaluate the effect of sildenafil, administered prior to renal ischemia/reperfusion (I/R), by scintigraphy and histopathological evaluation in rats. Methods: Twenty-four rats were divided randomly into two groups. They received 0.1 ml of 99mTechnetium-etilenodicisteine intravenous, and a baseline (initial) renal scintigraphy was performed. The rats underwent 60 minutes of ischemia by left renal artery clamping. The right kidney was not manipulated. The sildenafil group (n=12) received orally 1 mg/kg of sildenafil suspension 60 minutes before ischemia. Treatment with saline 0.9% in the control group (n=12). Half of the rats was assessed after 24 hours and half after seven days I/R, with new renal scintigraphy to study differential function. After euthanasia, kidneys were removed and subjected to histopathological examination. For statistical evaluation, Student t and Mann-Whitney tests were used. Results: In the control group rats, the left kidneys had significant functional deficit, seven days after I/R, whose scintigraphic pattern was consistent with acute tubular necrosis, compared with the initial scintigraphy (p<0.05). Sildenafil treatment resulted in better differential function of the left kidneys 24h after reperfusion, compared with controls. Histopathologically, the left kidney of control rats (24 hours after I/R) showed a higher degree of cellular necrosis when compared with the sildenafil treated rats (p<0.05). Conclusion: Sildenafil had a protective effect in rat kidneys subjected to normothermic I/R, demonstrated by scintigraphy and histomorphometry
ISSN: 1678-2674
Appears in Collections:CCS - DCIRUR - Artigos publicados em periódicos

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