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Title: Antioxidant fucoidans obtained from tropical seaweed protect pre-osteoblastic cells from hydrogen peroxide-induced damage
Authors: Fidelis, Gabriel Pereira
Silva, Cynthia Haynara Ferreira
Nobre, Leonardo Thiago Duarte Barreto
Medeiros, Valquíria Pereira
Rocha, Hugo Alexandre de Oliveira
Costa, Leandro Silva
Keywords: Bone cells;Sulfated polysaccharides;Osteoporosis;Brown seaweed;Oxidative damage
Issue Date: 28-Aug-2019
Publisher: MDPI
Citation: FIDELIS, Gabriel Pereira; SILVA, Cynthia Haynara Ferreira; NOBRE, Leonardo Thiago Duarte Barreto; MEDEIROS, Valquíria Pereira; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Antioxidant fucoidans obtained from tropical seaweed protect pre-osteoblastic cells from hydrogen peroxide-induced damage. Marine Drugs, [s. l.], v. 17, n. 9, p. 506-523, 28 ago. 2019. MDPI AG. Disponível em: Acesso em: 16 set. 2020.
Portuguese Abstract: Some antioxidant compounds decrease the amount of intracellular reactive oxygen species (ROS) and consequently reduce the deleterious effects of ROS in osteoblasts. Thus, these compounds fight against osteoporosis. Brown seaweeds are a rich source of antioxidant fucose-containing sulfated polysaccharides (fucans and fucoidans). We obtained six fucoidans (FRFs)—F0.3, F0.5, F0.7, F1.0, F1.5, and F2.1—from Dictyota mertensii by proteolytic digestion followed by sequential acetone precipitation. Except for F0.3, all FRFs showed antioxidant activity in different in vitro tests. In pre- osteoblast-like cells (MC3T3-L1) exposed to H2O2-oxidative stress, caspase-3 and caspase-9 were activated, resulting in apoptosis of the cells. We also observed a decrease in superoxide dismutase (SOD) and alkaline phosphatase (ALP) activity. The antioxidant FRFs protected the cells from the oxidative damage caused by H2O2, decreasing intracellular ROS and caspase activation, and increasing SOD activity. The most effective protection against damage was provided by F0.7, F1.5, and F2.1. At 0.5 mg/mL, these FRFs also suppressed the H2O2-mediated inhibition of ALP activity. The data indicated that FRFs F0.7, F1.5, and F2.1 from D. mertensii were antioxidants that protected bone tissue from oxidative stress and could represent possible adjuvants for the treatment of bone fragility through counteracting oxidative phenomena
ISSN: 1660-3397 (online)
Appears in Collections:CB - DBQ - Artigos publicados em periódicos
EMCM - Artigos publicados em periódicos

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