1. Universidade Federal do Rio Grande do Norte
2. Escola Multicampi de Ciências Médicas do Rio Grande do Norte
3. EMCM - Artigos publicados em periódicos

Title:	Aqueous leaf extract of Jatropha gossypiifolia L. (Euphorbiaceae) inhibits enzymatic and biological actions of Bothrops jararaca snake venom
Authors:	Félix-Silva, Juliana Souza, Thiago Menezes, Yamara A. S. Cabral, Bárbara Câmara, Rafael Barros Gomes da Silva-Junior, Arnóbio Antonio da Rocha, Hugo Alexandre de Oliveira Rebecchi, Ivanise Marina Moretti Langassner, Silvana Maria Zucolotto Fernandes-Pedrosa, Matheus de Freitas
Keywords:	Anticoagulants;Antidotes;Antioxidants;Blood Coagulation;Snakebites
Issue Date:	15-Aug-2014
Publisher:	Public Library of Science
Citation:	FÉLIX-SILVA, Juliana; SOUZA, Thiago; MENEZES, Yamara A. S.; CABRAL, Bárbara; CÂMARA, Rafael Barros Gomes da; SILVA-JUNIOR, Arnóbio Antonio da; ROCHA, Hugo Alexandre de Oliveira; REBECCHI, Ivanise Marina Moretti; LANGASSNER, Silvana Maria Zucolotto; FERNANDES-PEDROSA, Matheus de Freitas. Aqueous leaf extract of Jatropha gossypiifolia L. (Euphorbiaceae) inhibits enzymatic and biological actions of Bothrops jararaca snake venom. Plos One, [s. l.], v. 9, n. 8, p. e104952, 15 ago. 2014. Public Library of Science (PLoS). Disponível em: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0104952. Acesso em: 05 ago. 2020. http://dx.doi.org/10.1371/journal.pone.0104952.
Portuguese Abstract:	Snakebites are a serious public health problem due their high morbi-mortality. The main available specific treatment is the antivenom serum therapy, which has some disadvantages, such as poor neutralization of local effects, risk of immunological reactions, high cost and difficult access in some regions. In this context, the search for alternative therapies is relevant. Therefore, the aim of this study was to evaluate the antiophidic properties of Jatropha gossypiifolia, a medicinal plant used in folk medicine to treat snakebites. The aqueous leaf extract of the plant was prepared by decoction and phytochemical analysis revealed the presence of sugars, alkaloids, flavonoids, tannins, terpenes and/or steroids and proteins. The extract was able to inhibit enzymatic and biologic activities induced by Bothrops jararaca snake venom in vitro and in vivo. The blood incoagulability was efficiently inhibited by the extract by oral route. The hemorrhagic and edematogenic local effects were also inhibited, the former by up to 56% and the latter by 100%, in animals treated with extract by oral and intraperitoneal routes, respectively. The inhibition of myotoxic action of B. jararaca reached almost 100%. According to enzymatic tests performed, it is possible to suggest that the antiophidic activity may be due an inhibitory action upon snake venom metalloproteinases (SVMPs) and/or serine proteinases (SVSPs), including fibrinogenolytic enzymes, clotting factors activators and thrombin like enzymes (SVTLEs), as well upon catalytically inactive phospholipases A2 (Lys49 PLA2). Antiinflammatory activity, at least partially, could also be related to the inhibition of local effects. Additionally, protein precipitating and antioxidant activities may also be important features contributing to the activity presented. In conclusion, the results demonstrate the potential antiophidic activity of J. gossypiifolia extract, including its significant action upon local effects, suggesting that it may be used as a new source of bioactive molecules against bothropic venom
URI:	https://repositorio.ufrn.br/handle/123456789/31269
ISSN:	1932-6203 (online)
Appears in Collections:	CB - DBQ - Artigos publicados em periódicos CCS - DACT- Artigos publicados em periódicos CCS - DFAR - Artigos publicados em periódicos EMCM - Artigos publicados em periódicos

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